Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

In Vitro Regeneration Through Callus in Pointed Gourd (trichosanthes Dioica Roxb.)

M. A. MALEK
Plant Genetic Resources Centre, Bangladesh Agricultural Research Institute (BARI), Gazipur

M. A. MANNAN
Farm Division, Bangladesh Agricultural Research Institute (BARI), Gazipur

D. KHANAM
Biotechnology Division, Bangladesh Agricultural Research Institute (BARI),Gazipur, Bangladesh.

M. H. MOLLA
Biotechnology Division, Bangladesh Agricultural Research Institute (BARI),Gazipur, Bangladesh.

M. KHATUN
Biotechnology Division, Bangladesh Agricultural Research Institute (BARI),Gazipur, Bangladesh.

Abstract/ Executive Summary:

An efficient protocol was developed for in vitro plant regeneration and multiplication through callus culture in pointed gourd. Among the explants, highest percentage of cotyledon explants (92.00%) produced callus when this explant cultured in MS medium supplemented with NAA (0.1, 0.5, 1.0, 1.5, and 2.0 mg/l) and 2, 4-D (0.1, 0.5, 1.0, 1.5, and 2.0 mg/l). The highest number of shoots per explant was observed in MS + 0.5 mg/l BAP + 0.5 mg/l NAA followed by 1.0 mg/l BAP + 0.5 mg/l NAA when inter-node derived callus cultured in MS medium. Among the explants derived calli from leaf, inter-node and cotyledon in in vitro regeneration study, inter-node appeared as the most suitable explant for callusing and plant regeneration. The best response towards root induction was achieved on half MS medium supplemented with 0.5 mg/l NAA. The regenerated plantlets were successfully established in prepared earthen soil pot.

Keywords: In vitro regeneration, Pointed gourd.

Location: Plant Biotechnology Laboratory, Institute of Biological Sciences (IBSc.), University of Rajshahi, Rajshahi

Start Date:

End Date:

Program Area: Crop-Soil-Water Management

Commodity: Pointed gourd

Objectives:

the present investigation was conducted with a view to developing a protocol for plant regeneration through in vitro callus culture of pointed gourd.

Methodology:

Leaf, inter-node and root from in vitro grown plantiets and cotyledon generated from mature seed of pointed gourd were used as the explants for callus formation and plant regeneration. The experiments were conducted in the Plant Biotechnology Laboratory, Institute of Biological Sciences (IBSc.), University of Rajshahi, Rajshahi. The experiments were conducted following Completely Randomized Design (CRD). The data were analyzed for mean value and standard error (S. E). Inoculation: The excised leaf, inter-node, root, and cotyledons (obtained from mature seed of pointed gourd) were inoculated in each culture test tubes containing MS (Murashige and Skoog, 1962) media with various concentrations of 2,4-D (0.1, 0.5, 1.0, 1.5, and 2.0 mg/l) and NAA (0.1, 0.5, 1.0, 1.5, and 2.0 mg/l) and combinations of BAP (0.5 and 1.0 mg/l) with NAA (0.1, 0.5, 1.0, 1.5, and 2.0 mg/l) for callus formation. The pH of the medium was adjusted to 5.7 ± 0.1 using 0.1 N sodium hydroxide (NaOH) or 0.1 N HCI. In order to solidify the media, laboratory grade agar of 8.0 g (0.8%) was added to the solution. The culture tubes were plugged with aluminum foil and marked with glass marker pen to indicate specific hormonal supplement. The culture tubes were sterilized at 1.09 kg/cm2 pressure at 121°C for 15 minutes in an autoclave. After auto claving, the culture media were taken out and allowed to cool and solidify. For growth and development of cultures, the temperature was set at 25±1°C and a light intensity of 2000-3000 lux from fluorescent tubular lamps and the photoperiod was maintained generally at 16 hours light and 8 hours dark (16 L/8 D) cycle and relative humidity was 60-70%. The well rooted plantlets were then kept in room temperature for 2-3 days and transferred to polyethylene bags containing a mixture of soil, sand, and rice bran ash (1:1:1) and moist them adequately for proper hardening.

Source of Information: Bangladesh J. Agril. Res. 35(3) : 465-473, September 2010 ISSN 0258-7122

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Under the present study, efforts have been made to establish the protocol for regeneration of pointed gourd through callus under the treatment of different hormonal formulations. Multiple shoot propagation in pointed gourd (Trichosanthes dioica Roxb) was successfully developed to facilitate further advance research on genetic improvement through application of biotechnological approaches.

Knowledge Management: Journal