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In Vitro Growth and Development of Dendrobium Hybrid Orchid

H. KHATUN1
Department of Biotechnology, Bangladesh Agricultural University (BAU), Mymensingh

M. R. KABIR
Biotechnology Division, Bangladesh Agricultural Research Institute, (BARI), Gazipur, Bangladesh.

M. M. KHATUN2
Biotechnology Division, Bangladesh Agricultural Research Institute, (BARI), Gazipur, Bangladesh.

M. S. BISWAS
Biotechnology Division, Bangladesh Agricultural Research Institute, (BARI), Gazipur, Bangladesh.

M. AL-AMIN
Biotechnology Division, Bangladesh Agricultural Research Institute, (BARI), Gazipur, Bangladesh.

Abstract/ Executive Summary:

The experiment was conducted to investigate the combined effect of different plant growth regulators and charcoal supplementation in MS medium on growth and development of plantlets regenerated from protocorm like bodies (PLBs) of hybrid orchid. The combination of BAP + NAA, BAP + IAA, BAP + IBA, and IAA + IBA at different concentrations with charcoal supplementation was studied. The result revealed that the use of different growth regulators had significant effect on different parameters studied. The maximum weight of PLBs (5.123 g) was obtained from the combination of BAP + IBA at 1.0 mg/l each. The highest shoot height (3.239 cm) and maximum number of rooted plantlets (4.473) was obtained from 1.0 mg/l each of BAP + NAA combination. The maximum number of leaves (3.490) and the maximum length of leaves (1.946 cm) were obtained from 1.0 mg/l each of BAP + IBA and the highest leaf width (1.166 cm) was obtained from 0.5 mg/l BAP +1.0 mg/l IBA combination. The highest root length was obtained from 0.5 mg/l each of BAP + IAA and the maximum number of regenerated plantlets (20) was obtained from 0.5 mg/l IAA + 1.0 mg/l IBA combination. However, the maximum fresh weight of single shoot (0.219 g) and the maximum number of roots per plantlet (6.300) was obtained from 1.0 mg/I each of IAA + IBA combination.

Keywords: Dedrobium, Orchid, Hybrid, In vitro growth.

Location: The laboratory of Biotechnology Division, Bangladesh Agricultural Research Institute (BARI), Joydebpur, Gazipur.

Start Date:

End Date:

Program Area: Crop-Soil-Water Management

Non-commodity: Performance

Objectives:

The present study was undertaken to investigate which concentration and combination of growth regulators is better for plantlet regeneration and the subsequent growth and development of Dendrobium hybrid orchid with charcoal supplementation.

Methodology:

The experiment was carried out at the laboratory of Biotechnology Division, Bangladesh Agricultural Research Institute (BARI), Joydebpur, Gazipur. The protocorm like bodies (PLBs) were used as plant material which was obtained from a pod of Dendrobium hybrid orchid. Murashige and Skoog (1962) medium was used for culturing the planting materials. The MS medium was supplemented with different plant growth regulators (BAP, NAA, IAA, and IBA) and charcoal. Twenty grams of sucrose, 8.0 g of agar and 1.0 g charcoal were used per litre of the medium. The pH of the medium was adjusted to 5.8 before adding agar. The media were autoclaved at 1.1 kg/cm² pressure and 121°C for 20 mn. The inoculated cultures were incubated at 26 ± 1°C under approximately 2000 lux by cool-white fluorescent tubes for 16/8 light/dark cycle. Explants were subcultured at four weeks intervals or when necessary. Experiments were carried out in a completely randomized design (CRD) with three replications. The concentrations of growth regulators were used 0.5, 1.0, 2.0 mg/l in case of BAP, NAA, and IAA, while 1.0 mg/l in IBA and 1.0 g/l. charcoal. Data were collected at 60 days after inoculation (DAI) for weight of PLBs, height of shoot, number of leaves per plantlet, length of leaves, width of leaves, fresh weight of single shoot and number of regenerated plantlets per sample. Data collected were subjected to analysis of variance and means were separated by Duncan’s Multiple Range Test (DMRT).

Source of Information: Bangladesh J. Agril. Res. 35(3) : 507-514, September 2010 ISSN 0258-7122

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Considering the above results, the combination of BAP + IBA is the best for regeneration of Dendrobium hybrid orchid and the best concentration of each growth regulator was 1.0 mg/l.

Knowledge Management: Journal