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Nitrogen Uptake and Protein Yield in Lentil as Influenced by Seed Collection from Different Parts of Plants

A. KHATUN
Planning and Evaluation Division, Bangladesh Agricultural Research Institute (BARI), Gazipur

M. A. H. BHUIYAN
Soil Science Division, Bangladesh Agricultural Research Institute (BARI), Gazipur,

T.K. DEY
TCRC, Bangladesh Agricultural Research Institute (BARI), Gazipur, Bangladesh.

Abstract/ Executive Summary:

Field experiments were carried out during rabi (winter) seasons of 2004-2005 and 2005-2006 at the Bangladesh Agricultural Research Institute Farm, Gazipur to determine the effects of seeds collected from different plant parts on nitrogen content and nitrogen uptake, protein content, and protein yield of lentil. Lentil seeds were collected from different parts of lentil plants viz. i) seeds collected from upper parts (P1), ii) seeds collected from middle parts (P2), and iii) seeds collected from lower parts of lentil plants (P3). Significant variation among the three test varieties of lentil was observed for nitrogen content and nitrogen uptake, and protein content where the highest concentration of nitrogen was observed in BARI Masur-4 which ultimately gave higher protein yield, while the lowest was in BARI Masur-2. Seeds collected from different plant parts had significant effect for nitrogen and protein content where P3 site recorded the highest results. BARI Masur-2 seeds collected from lower parts of lentil recorded higher N content and protein content but BARI Masur-4 seeds collected from lower parts or BARI Masur-3 seeds collected from middle parts gave higher N uptake and protein yield.

Keywords: Lentil, Nitrogen uptake, Protein yield.

Location:

Start Date: 00-00-2003

End Date: 00-00-2004

Program Area: Crop-Soil-Water Management

Commodity: Lentil

Objectives:

The experiment was conducted to determine the effects of seeds collected from different plant parts on nitrogen content and nitrogen uptake, protein content and protein yield of lentil.

Methodology:

Experiments were carried out at the Research Field of the Pulse Research Centre, Bangladesh Agricultural Research Institute (BARI), Joydebpur, Gazipur, Bangladesh during the rabi (winter) seasons of 2004-2005 and 2005-06. During rabi season of 2003-04, lentil seeds of three popular varieties viz. BARI Masur- 2, BARI Masur-3, and BARI Masur-4 were collected from three sites of lentil plant viz. i) seeds collected from upper parts of lentil plant (P1 parts), ii) seeds collected from middle parts of lentil plant (P2 parts), and iii) seeds collected from lower parts of lentil plant (P3 parts). The collected seeds were preserved in earthen pot. Before setting up the field experiment, laboratory experiment was carried out for recording seed quality data (moisture percentage, germination percentage and vigour). The experimental field was high land with drainage and irrigation facilities. The area belongs to the Agro-ecological Zone-28 (AEZ 28) under Madhupur Tract. The experimental site is situated at 24.00° North Latitude and 90.26° East Longitude. The area received a long-term average rainfall (2142 mm) and average maximum monthly temperature was observed in April (32.6°C) and the minimum in December (11.2°C) (BARI, 2004). Field experiments were conducted using the collected seeds in a factorial randomized complete block design. Row to row distance was 30 cm and plant to plant distance was 10 cm. Unit plot size was 3 m × 4 m. Seeds were sown in 18 November 2004 and 11 November 2005, and harvested on 01 March 2005 and 25 February 2006, respectively. After harvesting, seeds of whole plot were dried to attain 10% moisture content and kept in polyethylene bag for determination of nitrogen concentration in seed. All the data were statistically analyzed using IRRISTAT package programme. Collection and preparation of seed samples for chemical analysis: Seed samples were collected from bulk harvest. The seed samples were then oven dried at 65°C for 24 hours and then ground. To obtain homogenous powder, the samples were passed through a 60-mesh sieve. The samples were stored in air tight polyethylene bags for nitrogen (N) determination (Page et al., 1982). Estimation of N: The N content in seed was determined by micro-Kjeldahl method. The seed samples (0.1 g seed) were digested with conc. H₂S0₄, hydrogen peroxide and K₂SO₄-catalyst mixture (K₂SO₄: CuSO₄. 5H₂O: Se = 10: 1: 0.1) at 200°C for one and a half-hour. Nitrogen was estimated by distilling the digest with 10N NaOH followed by titration of the distillate trapped in H₃B0₃ indicator solution with 0.0lN H₂SO₄ (Page et al., 1982). Calculation of protein content and protein yield: Protein content of lentil seed was determined by multiplying the N content of lentil seed with 6.25. Nitrogen uptake and protein yield of lentil seed was computed from the following formulae-Nitrogen uptake (kg/ha) = {Nitrogen content (%) × seed yield (kg/ha)}/100. Protein yield (kg/ha) = {Protein content (%) × seed yield (kg/ha}/100 Statistical analysis: The data for different characters were subjected to statistical analysis following a IRRISTAT package programme. The correlation co-efficient were done for different variables as needed.

Source of Information: Bangladesh J. Agril. Res. 35(3) : 515-523, September 2010, ISSN 0258-7122

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The overall results revealed that significant variation was observed in three varieties of lentil for nitrogen content, nitrogen uptake, protein content, and protein yield except N uptake and protein yield in 2005-06. Seeds collected from different sites of the plant had significant effect on nitrogen content and protein content. Seeds collected from lower parts (P3) recorded the highest nitrogen content, nitrogen uptake, protein content, and protein yield. Seeds collected from lower parts of BARI Masur-4 and seeds collected from middle parts of BARI Masur-3 recorded higher N uptake and protein yield.

Knowledge Management: Journal