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Effect of Storage Containers and Storage Periods on the Seed Quality of French Bean (phaseolus Vulgaris)

K. M. KHALEQUZZAMAN
Senior Scientific Officer
Bangladesh Agricultural Research institute (BARI), Ishurdi, Pabna.

M. M. RASHID
Professor
Department of Plant Pathology, Hajee Mohammad Danesh Science & Technology University (HSTU), Dinajpur.

M. A. HASAN
Associate Professor
Department of Agronomy, HSTU, Dinajpur.

M. M. A. REZA
Senior Scientific Officer
WRC, BARI, Nashipur, Dinajpur, Bangladesh.

Abstract/ Executive Summary:

The experiment was conducted in the Laboratory, Department of Plant Pathology, HSTU, Dinajpur during April – July 2010 to know the effect of abiotic and biotic factors, storage periods and storage containers on the seed quality of French bean. Tin container showed the highest germination, normal seedlings and vigour index which were followed by polythene bag, where Gunny bag showed the lowest germination, normal seedlings and vigour index upto 60 days after storage. The highest 1000-seed weight, moisture content, abnormal seedlings, seed rot and incidence of the Fusarium oxysporum were recorded in Gunny bag, where the lowest of these parameters were recorded in Tin container. Seed colour of Tin container were more or less same as initial colour, but seeds of Gunny bag were changed into fade or brown colour at 60 days after storage. Moisture content, 1000-seed weight, abnormal seedlings, seed rot, fungi association were increased, but germination and normal seedlings were decreased with the increase of storage periods. Among the three containers, Tin container was the best and the Gunny bag was the worst storage containers upto 60 days of storage for French bean seed.

Keywords: Storage containers, Storage periods, Seed quality, French bean

Location: Hajee Mohammad Danesh Science and Technology University (HSTU), Dinajpur

Start Date: 00-05-2010

End Date: 00-07-2010

Program Area: Postharvest and Agro-processing

Commodity: French bean

Objectives:

To determine the effect of abiotic and biotic factors, different storage periods and containers on the seed quality of French bean.

Methodology:

The experiment was conducted in the Laboratory, Department of Plant Pathology, Hajee Mohammad Danesh Science and Technology University (HSTU), Dinajpur, Bangladesht during April – July 2010. French bean seeds (var. BARI French bean 1) were collected from Plant Genetic Resource Centre (PGRC), RARS, Ishurdi, Pabna. The experiment was laid out in Completely Randomized Design (CRD) with four replications. Three storage containers- Tin container, Polythene bag and Gunny bag; and five storage periods - before storage, 15 days of storage, 30 days of storage, 45 days of storage, and 60 days of storage were used in this experiment. Seeds were stored at room temperature and relative humidity (RH) up to 60 days. The Tin container was covered tightly, and the Polythene bag and Gunny bag were tight with rope. Seed samples were taken randomly from each storage container were tested at five times i.e. five storage periods noted above. Moisture content was determined at every 15 days during experimental period. Moisture content was determined by using high constant temperature oven method (125-1300 C, 2 hr) following International Rules for Seed Testing (ISTA, 1999). Four hundred seeds were tested for germination from each container for every storage period in both the petridishes and the pots as per the rules of ISTA (1999). In petridish, moist blotter paper was used as germination media. Adequate moisture level was maintained in the germination media. In pot, germination test was conducted using sand as substratum. The sand was sieved to discard particles bigger than 0.8 mm and smaller than 0.05 mm in diameter. For every test new sand was used. Seed was placed on a uniform layer of moist sand and then covered to a depth of 10 mm with sand, which was left loose. The pot was irrigated at every odd day. After 5 days and 8 days, germination percentage was determined. Data on normal and abnormal seedlings, and shoot and root length were recorded from pot experiment. The normal seedlings and abnormal seedlings were classified according to the prescribed rules given by ISTA, 1999. For vigour index or seedling vigour data was recorded on germination up to 15 days of sowing. Then root length and shoot length were measured from seedlings of pots for calculating vigour index. Vigour index (VI) was calculated by using the formula of Baki and Anderson (1973) as shown below: Vigour index (VI) = Germination (%) X (Mean shoot length + mean root length) To detect the seed borne pathogens associated with the seeds in samples, the blotter method was used. The seeds in the petridish were inoculated at 20±20 C under alternating cycles of 12 hours near ultraviolet (NUV) light at darkness for 8 days. Each individual incubated seed was observed under Stereo-binocular microscope at 16 X and 25 X magnifications in order to record the incidence of seed borne fungi. Most of the associated pathogens were detected by observing their growth characters on the incubated seeds on blotter paper. For proper identification of fungi temporary slides were prepared from the fungal colony and observed under compound microscope and fungi were identified following the key outlined by Booth (1971) and Singh (1982). Insects and insect bitten seeds were observed for counting at the starting of experiment and at an interval of 15 days up to 60 days of storage. The data were recorded on 1000-seed weight, moisture content, germination, seed rotten, normal, and abnormal seedlings, vigour index, seed colour and prevalence of fungi and insect. The recorded data were analyzed statistically to find out the level of significance and the variations among the treatments were compared as per Duncan’s New Multiple Range Test (DMRT) (Gomez and Gomez, 1984).

Source of Information: Bangladesh J. Agril. Res. 37(2): 195-205, June 2012, ISSN 0258-7122

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Tin container showed the highest germination, normal seedlings and vigour index which were followed by polythene bag, where Gunny bag showed the lowest germination, normal seedlings and vigour index upto 60 days after storage. The highest 1000-seed weight, moisture content, abnormal seedlings, seed rot and incidence of the Fusarium oxysporum were recorded in Gunny bag, where the lowest of these parameters were recorded in Tin container. Seed colour of Tin container were more or less same as initial colour, but seeds of Gunny bag were changed into fade or brown colour at 60 days after storage. Moisture content, 1000-seed weight, abnormal seedlings, seed rot, fungi association were increased, but germination and normal seedlings were decreased with the increase of storage periods. Among the three containers, Tin container was the best and the Gunny bag was the worst storage containers upto 60 days of storage for French bean seed.

Knowledge Management: Journal