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Gonad Histology and Biological Aspects of Freshwater Gobi, Glossogobius Giuris (hamilton 1822)

Durin Akhter Jahan
Bangladesh Fisheries Research Institute, Freshwater Station, Mymensingh-2201, Bangladesh

Md. Shirajum Monir
Bangladesh Fisheries Research Institute, Head Quarter, Mymensingh-2201, Bangladesh

Md. Mominuzzaman Khan
Bangladesh Fisheries Research Institute, Freshwater Station, Mymensingh-2201, Bangladesh

A.H.M. Kohinoor
Bangladesh Fisheries Research Institute, Freshwater Station, Mymensingh-2201, Bangladesh

Abstract/ Executive Summary:

In this study, it was aimed at investigating the reproductive biology and histologically determining the developmental stages of oocytes of bele, Glossogobius giuris. Based on the weight of the gonad, presence or absence of ripe oocytes, diameter of oocytes in the ovary and histological observations: five typical gonad maturation stages were described for female using microscopic criteria. During observation period the mean fecundity was obtained 13909.57 ± 3574 and ovum length 0.032 mm to 0.054 mm. Based on the percentage of vitellogenic (VG) stages of oocytes and developmental pattern of gonadosomatic index (GSI) value, it was concluded that their breeding season restricted from April to June.

Keywords: Freshwater, Fecundity, Gobi, gonad histology, GSI

Location: Bangladesh Fisheries Research Institute

Start Date: 00-01-2010

End Date: 00-07-2010

Program Area: Animal Health and Management

Commodity: Finfish

Objectives:

To evaluate the GSI value based on reproductive biology and proper breeding of bele.

Methodology:

A total number of 200 live samples of gobi were obtained from Bramaputra river during November to December, 2009 and were temporarily stored at the indoor tanks of Bangladesh Fisheries Research Institute Hatchery Complex at Mymensingh and then kept in a pond. The fishes were fed with supplementary feed consisted with 35% protein and also live feed with small fish mola and prawn. Only gravid females /sexually mature fishes were used to study the fecundity, GSI and gonad development. To estimate GSI value and observation on gonad development was made on January to July, 2010. In each month ten fish samples were sacrificed. Prior to sacrifice the fish the total length were measured in the nearest mm and their weights were recorded in the nearest gram be a sensitive balance in the laboratory. All the samples were killed by over anaesthetized in ice water. After sacrificing the fish gonad were removed and then the length and weight of the ovaries were taken. Excess of water was removed from the surface of the fish as well as the ovaries with blotting paper before their weights were taken. To estimate the fecundity, gravimetric method was used and a portion of mature ovaries was wet weighed and the ripe oocytes were counted. For histological study the target organs (gonads) was sampled and preserve in Bouins fixative and then dehydrated by different grades of alcohol (70, 85, 96 and 99%). Samples were cleared by xylene and embedded in paraffin wax and then the wax blocks were sectioned. The sections were stained by hematoxyline and eosin and then subjected to a histological examination. The histological characteristics of G. giuris ovarian tissues were investigated. Gonads were classified according to the development with a four points of maturity: i).immature stage ii) maturing stage iii) mature stage iv) ripe stage. To estimate ova diameter 300 oocytes were measured from each sample in ripe stage. The oocyte was measured as maximum diameter using oculometer under a stage microscope.

Source of Information: Trends in Fisheries Research Volume 4, Issue 1 (2015) ISSN: 2319–474X (p); 2319–4758 (e)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Gobi, G. giuris is a semi-commercially important fish in Bangladesh. It is one of the sources of protein to the common people of Bangladesh. The outcome of the present study has provided some new and updated information on G. giuris. Knowledge about fecundity evaluates the commercial production and management of bele. A successful breeding programme as well as mass seed production of gobi, G. giuris in captive condition may save gobi from extinction. So, development of breeding programme of G. giuris is essential to ensure its availability in nature. The highest GSI value, beginning and peak period of vitellogenic stage of oocytes revealed the breeding period as well as spawning period of G. giuris lies between April and June.

Knowledge Management: Journal