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Reproductive Biology and Gonad Histology of Mud Eel, Monopterus Cuchia (hamilton, 1822)

Durin Akhter Jahan
Bangladesh Fisheries Research Institute, Freshwater Station, Mymensingh-2201, Bangladesh

Yahia Mahmud
Bangladesh Fisheries Research Institute, Freshwater Station, Mymensingh-2201, Bangladesh

Md. Mominuzzaman Khan
Bangladesh Fisheries Research Institute, Freshwater Station, Mymensingh-2201, Bangladesh

Jonaira Rashid
Bangladesh Fisheries Research Institute, Freshwater Station, Mymensingh-2201, Bangladesh

Abstract/ Executive Summary:

A study was performed to understand the reproductive biology and gonad histology of freshwater mud eel Monopterus cuchia for a period of 6 months. Gonadosomatic Index (GSI) and ova diameter were found to range between 0.31 to 1.85 and 0.53 mm to 4.20 mm, respectively. The GSI value chromonologically increased and found to peak in June (1.85). The highest ova diameter reached in June. Mean absolute and relative fecundity were 645.13±92.12 and 2.52±0.29, respectively. The percentage of Early Perinucleolus (EPN) stage was highest in March (7.67±1.53%) while Late Perinucleolus (LPN) stage was highest in April (31.00±2.65 %) and Cortical Alveoli (CA) stage appeared from April and reached to maximum in May (10.67±2.08%). Vitellogenic stage (VG) was found in ripe phase, prevailed from May to June and chronologically increased and highest percentage of ripe oocytes were recorded in June (33.67±3.51%).

Keywords: Threatened fish, Mud eel, GSI, Gonad histology, Fecundity

Location: Freshwater Station, Bangladesh Fisheries Research Institute, Mymensingh

Start Date: 00-01-2012

End Date: 00-06-2012

Program Area: Animal Health and Management

Commodity: Eel

Objectives:

1. This study will give information about reproductive biology of mud eel in Bangladesh ecological condition.

Methodology:

The present study was conducted for a period of six months from January to June 2012 in the Freshwater Station, Bangladesh Fisheries Research Institute, Mymensingh, Bangladesh. Live 400 M. cuchia samples weight varied from 200 to 400 g were collected from different area of Mymensingh district during November to December, 2011 and kept them in hapa for conditioning and then the eels were transferred in the cemented cistern where eight inches thick clay was used as soil layer. Live halencha and water hyacinth were used in cistern as shade and shelter for M. cuchia. The eels were fed with live tilapia fry, SIS and fish paste. After sunset fish paste was applied on the surface of water hyacinth. Collection of Gonad Sample - During experimental period M. cuchia was sexually separated, although reports are not available on sexual dimorphism of eels. M. cuchia there are no secondary sex characters for sexing. They identified male and female eels on external characters during breeding season. During the experimental period only female gonad was used. For the experiment ten gravid eels were randomly collected in each month from the cistern using plastic bowl where compost and earth warm were used. Collected eels were transferred to the laboratory and weight was recorded in gram. The peritoneal cavity was opened and collects the gonad. Collected gonads were preserved in Bouin’s fixative for further analysis. Determination Procedure of Breeding Parameters and Reproductive Biology - Fecundity, fertilization, hatching, incubation time, yolk sac absorption time and larval size are the breeding parameters. Among the breeding parameters fecundity and GSI value and ova diameter were observed according to the following method: Gonadosomatic Index (GSI) Gonads status and weight weighed on electronic balance before being preserved in Bouin’s fixative for subsequent studies. Total body weight of selected fishes in each month was considered to calculate the mean Gonadosomatic Index (GSI). GSI was calculated according to the formula. After dissecting the ovary, representative sample from each part of anterior, posterior and middle portion of the ovary was collected. The ova from each samples were separated using physiological saline solution (0.65% NaCl) in a petridish and then spreaded on a glass slide to measure the diameter of ova under a microscope using an ocular micrometer. The units of the ocular micrometer which were visible in the microscope were standardized with a stage micrometer for measurement of ova diameter in micrometer (μm). Approximately 100 ova from each sample were measured and determined the ova diameters. Histology of Gonad - The ovary tissues were fixed in aqueous Bouin’s fluid and then histological process was followed for slide preparation. The gonadal tissues were serially sectioned at a thickness of 4 μ and stained with hematoxylin-eosin. Nuclear diameters and physical feature of the oocytes were observed under microscope and different developmental stages were identified.

Source of Information: Int. J. Life Sc. Bt & Pharm Vol. 3, No. 1,. Res. 2014

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The variations in the gonad weight and GSI of the female cuchia fish reached to the peak during June indicating maturity of ovary and definite spawning season. While Vitellogenic stage (VG) of oocyte as well as highest ova diameter was observed in June also. The results of the present study may contribute to have successful breeding program and seed production technology of this fish species in captivity. Mass seed production and conservation of the available populations through proper management of the populations is recommended to save this threatened species from extinction.

Knowledge Management: Journal