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Effect of Enzyme and Vitamin Supplementation on Physio-biochemical Parameters in Broiler Chickens

S. Ahmed
Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh- 2202, Bangladesh

M. B. Rashid
Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh- 2202, Bangladesh

N. S. Lucky
Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh- 2202, Bangladesh

N. Ahmad
Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh- 2202, Bangladesh

M. Myenuddin
Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh- 2202, Bangladesh

Abstract/ Executive Summary:

The study was carried out on broilers to study the effect of oral administration of enzymes and vitamins on growth, haematological parameters and biochemical parameters in the Department of Physiology, Bangladesh Agricultural University for the period of 21 days from 15th January to 5th February 2007. A total of 20 broiler birds of 20 day old were divided into four equal groups as A, B, C and D. Group A was maintained as control and was fed only with commercial broiler ration through out the experimental period. Group B was treated with enzymes @ 1 gm per litre of drinking water, group C was treated with vitamins @ 0.5 gm per litre of drinking water and group D was treated with enzymes and vitamins combinedly at the rate of above mentioned dose. Body weight was recorded at 7 days interval up to the end of the 21 days of experimental period and the birds were sacrificed on the final day of experiment to collect blood sample for haematological (TEC, Hb, PCV and ESR) and biochemical study (SGOT and SGPT). Body weight was increased significantly (p < 0.01) in all the treated groups compared with the control group and highest was recorded in group D. TEC, PCV and Hb content increased significantly (p < 0.01) in the treated groups as compared to that of control group but ESR, SGOT and SGPT values decreased significantly (p < 0.01) in all the treated groups as compared to that of control group. The result reveals that use of enzymes and vitamins is an effective way of getting best result in terms of body weight gain and haemato-biochemical profiles in broiler birds. Key words:

Keywords: Broiler, Enzyme, Vitamin, Body weight, Haemato-biochemical parameters

Location: Begum Rokeya Prashickha Kendra, Maskanda, Mymensingh, Bangladesh.

Start Date: 00-01-2007

End Date: 00-02-2007

Program Area: Animal Health and Management

Commodity: Chicken

Objectives:

To investigate the effect of vitamins and enzymes on body weight gain and haemato-biochemical parameters in broiler chicks.

Methodology:

The birds were reared throughout the entire period of study in the well ventilated, well adapted room during the period from January to February 2007. The birds were then randomly divided into four equal groups, each consisting of 5 and numbered them as group A, B, C and D. Group A was maintained as control and was fed only with commercial broiler ration through out the experimental period. Group B was treated with enzymes @ 1 g per litre of drinking water, group C was treated with vitamins @ 0.5 g per litre of drinking water and group D was treated with combination of enzymes and vitamins at the rate of above mentioned dose from 0 to 21 days of experiment. Initial body weight of each bird was recorded just prior to segregation and kept them into cages. Body weight was recorded at 7 days interval up to the end of the 21 days of experimental period and the birds were sacrificed to collect blood sample for haematological (TEC, Hb, PCV and ESR) and biochemical study (SGOT and SGPT). Adequate feeders and waterers were provided to the birds. Proper humidity, temperature, hygiene and sanitary measures were also taken during the experimental period. Vaccination schedule was maintained properly. A number of sterile test tubes containing anticoagulant (4% sodium citrate) at a ratio of 1:10 was taken. Bood was collected from each group containing five birds through slaughtering at the end of the experiment. For preparation of serum samples, about 5 ml of blood was collected without anticoagulant in the sterile glass test tubes. The tubes containing blood were placed in a slanting position at room temperature for clotting. The tubes were then placed in the refrigerator at 4⁰C over night. Serum was then collected and was centrifuged at 1000 rpm for 15 minutes to get rid of unwanted substances. The serum was then stored in the screw capped serum vial and preserved at -20⁰C until use. The haematological studies were performed within two hours after the blood collection as per technique described. The SGOT and SGPT values were estimated. Analysis of variance was done with the obtained data according to the standard procedures.

Source of Information: Bangl. J. Vet. Med. (2007). 5 (1 & 2): 55–58

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

A total of 20 broiler birds of 20 day old were divided into four equal groups as A, B, C and D. Group A was maintained as control and was fed only with commercial broiler ration through out the experimental period. Group B was treated with enzymes @ 1 gm per litre of drinking water, group C was treated with vitamins @ 0.5 gm per litre of drinking water and group D was treated with enzymes and vitamins combinedly at the rate of above mentioned dose. Body weight was recorded at 7 days interval up to the end of the 21 days of experimental period and the birds were sacrificed on the final day of experiment to collect blood sample for haematological (TEC, Hb, PCV and ESR) and biochemical study (SGOT and SGPT). Body weight was increased significantly (p < 0.01) in all the treated groups compared with the control group and highest was recorded in group D. TEC, PCV and Hb content increased significantly (p < 0.01) in the treated groups as compared to that of control group but ESR, SGOT and SGPT values decreased significantly (p < 0.01) in all the treated groups as compared to that of control group. The result reveals that use of enzymes and vitamins is an effective way of getting best result in terms of body weight gain and haemato-biochemical profiles in broiler birds. This finding is supported by the above mentioned researchers. The better performance might be due to the synergistic action of both of them on the physiological system of the birds.

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