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In Vitro Regeneration Potentiality of Potato Under Different Hormonal Combination

M.E. Hoque
Department of Biotechnology, Sher-E-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka-1207 Bangladesh.

Abstract/ Executive Summary:

The present investigation was conducted to develop a protocol for rapid plant regeneration in potato. The newly emerge sprout of six potato cultivars were used as explant. Varietal response on in vitro regeneration under different hormonal concentration and combination were studied. MS medium supplemented with 0.5, 1.0, 1.5, 2.0, 4.0 mg L^-1 of KIN and IAA in combination of both were employed for in vitro regeneration. Among the different treatments the combination 2.0 mg L^-1 of KIN and IAA showed the best response to multiple shoot and root regeneration. The same concentration also takes minimum time for regeneration. The interaction between hormonal concentration and varietal potentiality for all the parameter showed significant difference at 5% level of probability. The in vitro regeneration and multiplication potentiality was the highest in the variety Granulla followed by Cardinal and Diamont.

Keywords: Potato, Indole acetic acid (IAA), Kinetin(KIN), Regeneration

Location: Department of Biotechnology, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka, Bangladesh

Start Date: 00-09-2008

End Date: 00-06-2009

Program Area: Variety and Species

Commodity: Potato

Objectives:

To identify the best hormonal combination and concentration for potato regeneration.

Methodology:

The present study was carried out at the Department of Biotechnology, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka, Bangladesh, during the period September, 2008 to June, 2009. The experimental materials (six potato varieties) were collected from Tuber Crops Research Center (TCRC), Bangladesh Agricultural Research Institute (BARI), Gazipur-1701, Bangladesh. The cultivars are as follows- Diamant, Cardinal, Granulla, Ultra, Dheera and Provinto. Disease-free healthy potato tuber were treated with 50 ppm GA for sprouting. The sprouted 3 buds were used as explant. The experiment was design on two factor factorial condition. The different hormonal concentration was treated as factor 1 and the different varieties of potato were used as factor 2. The experiment was laid out in Completely Randomized Design (CRD) with 3 replications. Ten explants were considered in each treatment and in each varieties. The sprouts were surface sterilized by immersing in 0.2% HgCl₂ solution for 2-3 minand then washed several times with sterile distilled water. The explant (sprouts) were cultured in MS semi-solid medium (pH 5.8) on different culture vasel. The medium were autoclaved at 121°C with 1.16 kg cm^-2 pressure for 25 minute. The treatment concentration of Kinetin (KIN) and Indole Acetic Acid (IAA) were viz. 0.5, 1.0, 1.5, 2.0 and 4.0 mg L^-1. The concentration were applied in combination of both the hormone. The cultures were incubated at 25±2°C under white light intensity (2500-3000 Lux). Data were recorded on the following parameters, days to shoot regeneration, number of shoot per explant, days to root induction, number of root per explant. Two factor factorial analysis of variance were performed and mean were compared by the Duncan’s Multiple Range Test (DMRT).

Source of Information: World Journal of Agricultural Sciences 6 (6): 660-663, 2010, ISSN 1817-3047

Article Link (Online Journal): http://www.idosi.org/wjas/wjas6(6)/5.pdf

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Among the different treatments the combination 2.0 mg L-1 of KIN and IAA showed the best response to multiple shoot and root regeneration. The same concentration also takes minimum time for regeneration. The interaction between hormonal concentration and varietal potentiality for all the parameter showed significant difference at 5% level of probability. The in vitro regeneration and multiplication potentiality was the highest in the variety Granulla followed by Cardinal and Diamont.

Knowledge Management: Journal