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Performance of Different Protocols on in Vitro Tuberization in Potato (solanum Tuberosum)

M. ZAKARIA
Associate Professor
Department of Horticulture, Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur

M. M. HOSSAIN
Professor
Department of Horticulture, BSMRAU, Gazipur

M. A. KHALEQUE MIAN
Professor
Department of Genetics and Plant Breeding, BSMRAU, Gazipur

T. HOSSAIN
Professor
Department of Crop Botany, BSMRAU, Gazipur, Bangladesh.

Abstract/ Executive Summary:

Five protocols of micro tuberization were used to induce large size microtuber in three recommended potato varieties, namely Cardinal, Diamant, and Heera under complete dark condition. Tuberization was the earliest (11.8 days) in the protocol P2 (MS + 5 mg/l BAP + 500 mg/l CCC + 8% sucrose), which was closely followed by that in P1 (12.7 days) (MS + 5 mg/l BAP + 50 mg/l coumarin + 8% sucrose). Maximum number of microtubers/flask (12.8) was obtained from the protocol P1 followed by that of P2 (11.6) that contained growth retardant; but higher average weight of microtuber was obtained in the protocols P5 (30 days old plantlet + MS media containing 40 meq K + 10 mg/l BA + 9% sucrose), P4 (MS + 10 mg/l BA + 8% sucrose), and P3 (MS + 5.0 mg/l BAP + 6% sucrose) which contained BA in absence of growth retardant. The average weight of microtuber was the highest (329.0 mg) in protocol P5, followed by that in P4 (280.7 mg), while it was the lowest in protocol P1. The variety Diamant produced maximum average weight of microtuber (246.3 mg), while Heera produced minimum (226.1 mg), which was statistically similar to Cardinal (228.7 mg). The highest percentage (52.2) of >300 mg size and lowest percentage (19.3) of <150 mg size microtuber was produced in P5 protocol in the variety Diamant. On overall consideration, all the varieties performed best with the protocol P5.

Keywords: Protocol, Microtuber, Potato.

Location: Tissue culture laboratory of Horticulture Department, Bangabandhu Sheikh Mujibur Rahman Agricultural University.

Start Date: 00-07-2008

End Date: 00-09-2008

Program Area: Variety and Species

Non-commodity: Performance

Objectives:

The present study was undertaken to evaluate the performance of different protocols on in vitro tuberization in different potato varieties and to find out variety specific protocols.

Methodology:

In vitro grown microplants of three recommended potato cultivars viz., Cardinal, Diamant and Heera were used in the study. The microplants were multiplied through subculture of single stem nodes at every three weeks interval in Tissue culture laboratory of Horticulture Department, Bangabandhu Sheikh Mujibur Rahman Agricultural University. The experiment was carried out during the period from July to September 2008. The propagation media used for different protocols. All the propagation media were solidified with 0.8% agar. Temperature of the growth chamber was maintained at 23 +1^oC with 16 hours photoperiod at 3000 lux intensity from fluorescent tubes. At first phase, microplants were multiplied in solid media and then these were transferred into liquid media for microtuberization. Eight stem segments (Each with 3 nodes) of 30 days old in vitro microplants of Cardinal, Diamant, and Heera were cultured in 250 ml Erlenmeyer flasks containing 40 ml microtuber induction liquid medium, which was based on MS medium supplemented as per different protocols. The cultures were incubated in the dark at 20^oC in continuous dark. The experiment included five protocols and three recommended potato varieties which in combination made 15 treatment combinations. The experiment was laid out in a factorial Completely Randomized Design (CRD) with three replications. The induced microtubers were harvested aseptically after 70 days of incubation. The collected data were analyzed with the help of computer using MSTAT-C programme and the mean separation was done by Duncan’s New Multiple Range Test.

Source of Information: Bangladesh J. Agril. Res. 39(1): 59-66, March 2014 ISSN 0258-7122

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The highest percentage (52.2) of >300 mg size and lowest percentage (19.3) of <150 mg size microtuber was produced in P5 protocol in the variety Diamant. On overall consideration, all the varieties performed best with the protocol P5.

Knowledge Management: Journal