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Antimicrobial, Antioxidant and Cytotoxic Properties of Hypsizygus Tessulatus Cultivated in Bangladesh

S. Monira
Department of Pharmacy, University of Rajshahi, Rajshshi 6205, Bangladesh.

A. Haque
Department of Pharmacy, University of Rajshahi, Rajshshi 6205, Bangladesh.

A. Muhit
Department of Clinical Pharmacy and Pharmacology, University of Dhaka, Bangladesh.

N. C. Sarker
National Mushroom Development and Extension Centre, Sobhanbag, Dhaka, Bangladesh.

A. H. M. K. Alam
Department of Pharmacy, University of Rajshahi, Rajshshi 6205, Bangladesh.

A. A. Rahman
Department of Pharmacy, University of Rajshahi, Rajshshi 6205, Bangladesh.

P. Khondkar
Department of Pharmacy, University of Rajshahi, Rajshshi 6205, Bangladesh.

Abstract/ Executive Summary:

The aim of the present study was to evaluate certain biological properties of Hypsizygus tessulatus. Metabolic extract of submerged culture and mycelia mats of H. tessulatus mycelium was taken for investigating cytotoxic and antimicrobial activity using brine shrimp lethality bioassay and disc diffusion technique, respectively. Ethyl acetate extract showed higher antimicrobial activity than chloroform extract against the Gram positive and Gram negative bacteria. The average zone of inhibition for ethyl acetate extract was in the range of 17-22 mm. Ethanol extract of mycelia mats of H. tessulatus showed significant antioxidant property using 1, 1-diphenyl-2-pecrylhydrazyl (DPPH) scavenging assay along with total phenol content demonstrated moderate activity with IC₅₀value of 105 μg mL^-1 and 36.45±1.754 g/100 g, respectively. The total antioxidant capacity of H. tessulatus was 442.61 mg equivalents of ascorbic acid. In brine shrimp lethality bioassay, LC₅₀values obtained from the best fit line slope were 7.36 and 10.63 μg mL^-1 for ethyl acetate and chloroform extract, respectively. The study reveals that H. tessulatus mushroom has potential antimicrobial effect with moderate anticancer and antioxidant activity.

Keywords: Antioxidant, Antimicrobial activity, Mushroom

Location: National Mushroom Development and Extension Centre, Dhaka.

Start Date: 00-00-2013

End Date: 00-00-2014

Program Area: Development of Host and Medicinal Plants

Commodity: Medicinal Plants

Objectives:

To evaluate the antibacterial, cytotoxic and antioxidant properties of Hypsizygus tessulatus different extract both from submerged culture and mycelia mat.

Methodology:

Hypsizygus tessulatus were collected from National Mushroom Development and Extension Centre, Dhaka. The mushroom was obtained as potato dextrose agar slants. This mushroom was reported by the supplier to grow at 25°C. The species was maintained on agar slants at 4°C and sub-cultured every twelve weeks according to the supplier’s recommendation. Three mycological media were used to evaluate the optimum growth of the organism. The organism was sub-cultured on potato dextrose agar or broth (PDA/PDB), malt-yeast extract (MY) and glucose-yeast extract peptone (GPY) media. Potato dextrose and glucose-yeast extract peptone media were sterilized at 121°C for 15 min whereas, malt-yeast extract medium was sterilized at 115°C for 10 min. All these media were purchased from Oxiod and Difco, UK. The Hypsizygus tessulatus was culture in potato dextrose broth (PDB) medium, as maximum growth was observed in this medium (data not shown). The submerged culture was divided into two parts after 10 days of incubation at 28°C. The mycelia which formed a mat like structure was separated by filtration (Whatman No.1) and both parts were collected for further study. The isolated mats were air dried in an oven at 40°C before analysis and pulverized to fine powder by using motor and pestle. Dried mat (40 g) was extracted by stirring with 300 mL of ethanol at 30°C at 150 rpm for 24 h and filtering through Whatman No. 1 filter paper. The residue was then, extracted with two additional 300 mL of ethanol as described above. The combined ethanolic extract was then rotary evaporated at 40°C to dryness. Three replicates of each sample were used for statistical analysis and the values were reported as Mean±SEM. Correlation analysis of free radical scavenging activity versus total phenolic content and cytotoxicity were carried out using the correlation and regression program.

Source of Information: Research Journal of Medicinal Plants, 6(4): 300-308, 2012, ISSN 1819-3455

Article Link (Online Journal): DOI: 10.3923/rjmp.2012.300.308; URL: http://scialert.net/abstract/?doi=rjmp.2012.300.308

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Medicinal mushrooms used in the traditional medicine may be an interesting and largely unexplored source for the development of potential new compounds while the pragmatic medicinal properties have been attributed to mushrooms for thousands of years. But it is necessary to isolate the active principles and characterize their constituents for the benefit of human being. In conclusion, the metabolite and mycelia mat extracts of H. tessulatus demonstrated moderate antimicrobial, antioxidant activity, free radical scavenging activity as well as strong cytotoxic activity.

Knowledge Management: Journal