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Micropropagation of Strawberry (fragaria Ananassa) Through Runner Culture

M. ASHRAFUZZAMAN
Dept. of Genetic Engineering and Biotechnology, School of Life Sciences, Sylhet University of Science & Technology, (SUST), Sylhet-3114

S. M. FAISAL
Horticulture Division, RARS, Bangladesh Agricultural Research Institute (BARI), Hathazari, Chittagong,

D. YADAV
Dept. of Genetic Engineering and Biotechnology, School of Life Sciences, Sylhet University of Science & Technology, (SUST), Sylhet-3114

D. KHANAM
Biotechnology Division, BARI, Gazipur-1701,

F. RAIHAN
Dept. of Forestry and Environment, Sylhet University of Science & Technology, (SUST), Sylhet-3114

Abstract/ Executive Summary:

In vitro propagation of strawberry was conducted at the Biotechnology Lab. of BARI, Joydebpur, Gazipur. For shoot induction, five BAP concentrations viz., 0.0 (Control), 0.5, 1.0, 1.5, and 2.0 mg/l and for root induction four IBA concentrations viz., 0.0 (Control), 0.5, 1.0, and 1.5 mg/l were used. The highest average number of shoots (7) and the highest average length (3.34 cm) of shoot was observed at the concentration of 0.5 mg/l BAP. The highest average number of leaves (5) was also observed at the same concentration. Among the five rooting concentrations, IBA @ 0.5 mg/l showed the best performance in all the parameters studied. The highest number (6) of roots/culture and the longest (3.05 cm) roots were also obtained from this concentration. Half strength MS media without IBA concentration did not show any response regarding root induction.

Keywords: Proliferation, Micropropagation, Strawberry, Inoculation.

Location: Biotechnology Laboratory of BARI, Joydebpur, Gazipur, Bangladesh

Start Date: 00-03-2010

End Date: 00-09-2010

Program Area: Seed Technology

Commodity: Strawberry

Objectives:

The significant advantages of micropropagation by which a large number of plants can be produced from a single individual in a relatively short span of time and space. Keeping in mind the above facts, the present research work was conducted.

Methodology:

The experiment was carried out at the Biotechnology Laboratory of BARI, Joydebpur, Gazipur, Bangladesh during March -Septemper 2010. For shoot induction, five BAP concentrations viz., 0.0 (Control), 0.5, 1.0, 1.5, and 2.0 mg/l and for root induction, four IBA concentrations viz., 0.0 (Control), 0.5, 1.0, and 1.5 mg/l were used. Both in shoot and root induction experiment, each treatment consisted one test tube and replicated five times and average number of multiple shoots and roots were counted. A nutrient medium for plant regeneration usually consists of organic and inorganic salts, irons, a carbon source, some vitamins and growth regulators. In this study, MS (Murashige and Skoog, 1962) medium was used as basal medium for plant regeneration. Runner segments and runner tips of BARI Strawberry -1 was used as explant. Explants were collected from Horticulture Research Centre (HRC) of BARI. For establishing the explants in the media, only the tender and actively growing runner segments and runner tips having 1.00-1.25 cm long were prepared to use as explants. The explants were washed with running tap water and sterilized with trix and HgCl2. To maintain aseptic condition, precautions were taken in every step of works. All inoculations and aseptic manipulations were carried out in a laminar airflow cabinet. During operation, hands and cabinet base were rubbed with 70% ethyl alcohol frequently for maintaining clean condition. To obtain possible contamination free condition in clean bench, proper care was taken during explant preparation. Shoot tips were prepared inside the laminar airflow cabinet using a fine sterile forcep and scalpel. The excised shoot tips were then inoculated on to the culture test tubes per vials containing various concentrations of BAP for in vitro multiple shoot regeneration. The physical conditions for growth and development of cultures were maintained at the temperature of 25 ± 10C and a light intensity of 2000-3000 lux provided by fluorescent tube. The photoperiod was maintained at 16 hours light and 8 hours dark (16L/8D) and the relative humidity was 60-70%. Successful shoot formation became evident when small green fresh leaves began to emerge. It is the first sign of regeneration. These tiny leaves, when developed in their actual shape were transferred into fresh media containing the same hormonal concentration for further proliferation and development of shoots. First subculture and second subculture were carried out at three and five weeks after shoot initiation, respectively. The regenerated multiple shoots were carefully removed from the test tubes and placed on a sterile hard paper. Each shoot was cut from the basal end and was transferred to new media containing concentrations of BAP for further multiple shoot induction. For root induction, half strength MS media fortified with different IBA concentrations were used. Data on number of explants cultured, average number of shoots/culture, average length of shoots/culture, average number of shoots per culture, days to root induction, average number of roots per culture and average length of roots/ were recorded. Mean data were calculated to present the statistical information.

Source of Information: Bangladesh J. Agril. Res. 38(3): 467-472, September 2013, ISSN 0258-7122

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

present results as root formation was observed within 2 weeks of plantlet inoculation. No rooting was observed in auxin free media and the best response of root formation was observed at 0.5 mg/l IBA enriched media. In this investigation, a micropropagation protocol for strawberry has been developed. On the other hand, this finding could be utilized in plant genetic transformation studies for better improvement.

Knowledge Management: Journal