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Bacteriological, Physical and Chemical Properties of the Pagla Sewage Treatment Plant’s Water

MIHIR LAL SAHA
Laboratory of Microbiology, Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh

ASHRAFUL ALAM
Laboratory of Microbiology, Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh

MAHBUBAR RAHMAN KHAN
Laboratory of Microbiology, Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh

SIRAJUL HOQUE
Department of Soil, Water and Environment, University of Dhaka, Dhaka-1000, Bangladesh.

Abstract/ Executive Summary:

Samples from Pagla sewage treatment plant at different treatment stages showed more or less similar temperature (26.9 ? 27.50C). The pH ranged from 7.2 ? 7.9. Influent water and primary sedimentation tank water were brownish in colour while sludge water was light black. The lagoon water and treated water were greenish. The ammonium?nitrogen (NH+4?N) and nitrate?nitrogen (NO?3?N) ranged from 5.24 ? 61.94 mg/l and 2.55 ? 11.02 mg/l, respectively. Phosphorus of the water was 1.34 ? 4.50 mg/l. The suspended solids (SS) ranged from 25.48 ? 374.69 mg/l. In the present study the amount of SS in the treated water were found to be quite satisfactory. The total bacterial population in Pagla sewage treatments plant was in between 2.9 × 104 and 2.5 × 106 cfu/ml. The qualitative bacterial spectrum showed a potential consortium of bacteria associated with the treatment plant. Both Gram positive and Gram negative bacteria were present. Gram positive bacteria were represented by the different species of the genus Bacillus, Aureobacterium and Kurthia. Among them Bacillus was the dominant genus. The different species of Bacillus were Bacillus sphaericus, B. fastidiosus, B. circulans and B. pasteurii. Gram negative bacteria were Zoogloea, Yersinia, Citrobacter and Pseudomonas. A good number of microorganisms were found to be associated with the bio?oxidation of the organic compounds of the influent. The Zoogloea along with other free flowing aerobic heterotrophic bacteria like Bacillus, Pseudomonas could play the major role in the sewage treatment.

Keywords: Bacteria, Chemical properties, Treatment plant, Sewage

Location: Pagla wastewater treatment plant, Pagla, Narayanganj, Dhaka, Bangladesh.

Start Date: 00-00-2012

End Date: 00-00-2012

Program Area: Crop-Soil-Water Management

Commodity: Water quality

Objectives:

1. The present work was undertaken to determine the physical and chemical nature and the bacteria associated with the wastewater and treated water of the wastewater treatment plant of Pagla, Narayanganj, Dhaka, Bangladesh.

Methodology:

Water samples were collected from five different stages viz. influent, primary sedimentation tank, sludge water, lagoon water, treated water of Pagla wastewater treatment plant. The colour and temperature of the samples were recorded carefully at the time of sampling while pH of the samples was measured in the laboratory with an electric pH meter (Jenway 3310, U.K.) immediately. Samples were preserved in a refrigerator at 4ºC before and after the microbial analysis. The isolation of bacteria was done as soon as possible after collection of samples. The bacterial colonies were isolated by decimal dilution plate procedure. For isolation of aerobic heterotrophic bacteria, nutrient agar (NA) medium was used and the pH of the medium was adjusted to 7.0. After 24 hours of incubation, the plate having 30 ? 300 colonies were selected for counting and isolation of bacteria. The selected plates were placed in a colony counter (Digital colony counter, DC?3, OSK 10086, Kayagaki, Japan) and the colonies were counted. The viable bacterial count per ml was calculated by multiplying the average number of colonies per plate by the reciprocal of the dilution. The results would be as colony forming units cfu/ml water sample. Isolation of the selected colonies was carried out immediately after counting the colonies. The selected isolates were then purified through repeated streaking on nutrient agar plate. Biochemical and physiological tests were carried out for the identification of the isolated bacteria. For the estimation of suspended solids (SS) the collected samples were centrifuged for 10 minutes at 10,000 rpm. The precipitations were taken in the porcelain cups and dried in an oven at 100ºC till constant weight. Suspended solids were calculated by subtracting the initial weight from final weight. Ammonium?nitrogen and nitratenitrogen of the samples were determined following Kjeldahl’s distillation method and colorimetric method, respectively. Phosphorus present in the samples was determined by ascorbic acid blue colour method.

Source of Information: Dhaka Univ. J. Biol. Sci. 21(1): 1&7, 2012 (January)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

It can be concluded that a good number of microorganisms were found to be associated with the bio?oxidation of the influent organic compounds. One of the important floc forming bacteria Zoogloea was present among the bacteria isolated. The genus Zoogloea along with other free flowing aerobic heterotrophic bacteria could play important role in the treatment of the domestic wastewater of the plant.

Knowledge Management: Journal