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Plasma Pharmacokinetics of Ciprofloxacin in Sheep

M. S. Islam
Department of Pharmacology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

M. M. H. Sikder
Department of Pharmacology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

M. A. Awal
Department of Pharmacology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

M. Mostofa
Department of Pharmacology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

A. A. Trisha
Department of Pharmacology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Abstract/ Executive Summary:

The study was carried out to determine the biodisposition kinetics of ciprofloxacin in sheep model in Department of Pharmacology, Bangladesh Agricultural University. Healthy sheep of both sexes (n=65) were divided into 13 groups, each consists of five and given a single dose of ciprofloxacin @ 5 mg/kg bwt intramuscularly .Blood sample was collected from each group of sheep at 0, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 6, 8, 10 and 12 hours interval respectively. Serum concentration of ciprofloxacin was determined by spectrophotometric method. The pharmacokinetic parameters were measured by single compartment open model and first order kinetics. The peak concentration of ciprofloxacin was 3.56±0.15μg/ ml, absorption half-life and biological half-life were 0.0846±1.79 and 1.75±0.15 h respectively. The apparent volume of distribution was found 35.54 mg/liter. The absorption rate constant was 8.188h-1, MRT was 2.647h-1 and total body clearances were found 16.88 h-1. These result suggested that a dose of 5 mg/kg bwt provides maximum plasma concentration and is effective in the control of many infectious diseases of sheep.

Keywords: Plasma pharmacokinetics, Ciprofloxacin, Sheep

Location: Experimental pharmacological laboratory, Department of Pharmacology, Bangladesh Agricultural University, Mymensingh, Bangladesh.

Start Date:

End Date:

Program Area: Animal Health and Management

Commodity: Sheep

Objectives:

To determine the biodisposition kinetics of ciprofloxacin in sheep

Methodology:

The experiment was performed in 65 clinically healthy native sheep. The animal was under the Department of Pharmacology, Bangladesh Agricultural University. The animals was provided with normal feed and water to get acclimatized for 8 weeks before the study to make sure that none of them had received any medication for this period. All sheep were kept in good housing. They were divided into 13 groups (A-M) with five sheep in each group. Ciprofloxacin (Cipryl® 50 ml, 1 ml contain 50 mg ciprofloxacin) was administered intramuscularly at the rate of 5mg/kg body weight with the help of syringe and needle. 5 ml of blood samples were collected from jugular vein of each sheep at 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0, 6.0, 8.0, 12.0 hour interval strictly under aseptic conditions. Collected blood sample was transferred into hematocrit tube and it was allowed to stand for 20 minutes and then centrifuged at 4000 rpm for 15 minutes, serum was separated and stored at –20⁰C till further analysis. Ciprofloxacin concentration was measured by UV-spectrophotometeric revised method. The method involves deproteinization of plasma samples by using isopropyl alcohol and measuring the absorbance at 280 nm. Since isopropyl alcohol gave high absorbance of its own in the blank samples therefore the method was modified and improved. Before running the plasma samples standard curve for Ciprofloxacin was made. The data was analyzed statistically by ‘t’ test described.

Source of Information: Bangl. J. Vet. Med. (2008). 6 (1): 93–97

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The pharmacokinetic parameters were measured by single compartment open model and first order kinetics. The peak concentration of ciprofloxacin was 3.56±0.15μg/ ml, absorption half-life and biological half-life were 0.0846±1.79 and 1.75±0.15 h respectively. The apparent volume of distribution was found 35.54 mg/liter. The absorption rate constant was 8.188h^-1, MRT was 2.647h^-1and total body clearances were found 16.88 h^-1. These result suggested that a dose of 5 mg/kg bwt provides maximum plasma concentration and is effective in the control of many infectious diseases of sheep. In conclusion, ciprofloxacin @ 5mg/kg body weight intramuscularly gives some variable pharmacokinetics parameters with regard to cited literature in native sheep and further investigation should carry out to find out the cause of variations.

Knowledge Management: Journal