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Antifungal Potentials of Derris Indica (lam.) Bennet Extractives

Omar Ali Mondal
Institute of Biological Sciences, University of Rajshahi, Rajshahi 6205, Bangladesh

K.A.M.S.H. Mondal
Institute of Biological Sciences, University of Rajshahi, Rajshahi 6205, Bangladesh

Nurul Islam
Department of Zoology, University of Rajshahi, Rajshahi 6205, Bangladesh.

Abstract/ Executive Summary:

Antifungal activity of the D. indica (Lam.) Bennet. extractives collected in CHCl₃ and methanol were tested against seven pathogenic fungi F. vasinfectum, A. fumigatus, A. niger, A. flavus, Mucor sp., C. albicans and P. notatum at concentrations of 50 and 200 µg/disc along with a standard Nystatin (50 µg/disc). The fruit shell extract showed activity index against C. albicans and P. notatum. The leaf and the root bark extracts were responsive on A. fumigatus, C. albicans, P. notatum and F. vasinfectum. For the root wood extract F. vasinfectum, A. fumigatus,, C. albicans were responsive. For the seed, stem bark and stem wood extract showed activity index against A. flavus, C. albicans, A. fumigatus P. notatum and Mucor sp. According to the intensity of activity indices D. indica extracts (CHCl₃) could be arranged in a descending order of fruit shell > leaf > root bark > root wood > seed >stem wood> stem bark extract. For the MeOH extracts the fruit shell, leaf, root bark and root wood extracts showed activity indices against A. fumigatus, C. albicans , P. notatum , Mucor sp., F vasinfectum, and A. flavus. In case of the seed, stem bark and stem wood extracts A. flavus, C. albicans, A. fumigatus, P. notatum and Mucor sp. were responsive. According to the intensity of activity indices D. indica extracts (MeOH) could be arranged in a descending order of fruit shell > leaf > root bark > root wood >seed> stem bark > stem wood extract.

Keywords: Chloroform and Methanol extract, Derris indica, Antifungal activity.

Location: Molecular Biology Laboratory, IBSc, RU.

Start Date:

End Date:

Program Area: Development of Host and Medicinal Plants

Commodity: Medicinal Plants

Objectives:

The present investigation was aimed at evaluating chloroform and methanol extracts of some selected parts of D. indica against a number of pathogenic fungi using disc diffusion method.

Methodology:

The test fungi and their culture: Pure cultures of seven species of fungi viz., Fusarium vasinfectum, Aspergillus fumigatus, Aspergillus niger, Aspergillus flavus, Mucor sp., Candida albicans and Penicillium notatum were collected from the Department of Pharmacy, Rajshahi University (RU), and were maintained in the Molecular Biology Laboratory, IBSc, RU. Potato dextrose agar (PDA) media, consisting of 20g potato, 2g dextrose, 1.5g agar and 100 ml distilled water, were used to perform the antifungal activity tests and for the maintenance of the subcultures of the test organisms. The constituents of the media were accurately weighed and dispersed in a conical flask with distilled water. It was heated in water bath to dissolve the ingredients until a transparent solution was obtained. The pH of the medium was adjusted to 5.6. The volume was adjusted by adding distilled water and sterilized in an autoclave at 121° C and 15 Ib. inch-2 pressures for 15 min. Plant parts and their extraction: The fresh leaves, fruit shell, seeds, stem bark, stem wood, root bark and root wood of D. indica were collected from the RU Campus. After drying under shade, the plant materials were powdered in a grinder separately avoiding excess heat during grinding. The ground dried plant materials were extracted with sufficient amount of chloroform and methanol (500g × 1500ml × 3 times) for each of the items. Separate extracts were collected by the cool method after 72 hours of plunging for each of the materials. Extracts thus obtained through filtration and evaporation of the solvent as residue were kept in a refrigerator until used. Tests for antifungal activity: The agar diffusion technique (Vander and Vlietinck 1991) was employed to conduct antifungal screening while PDA medium was used for determining antifungal activity (Bauer et al. 1966) of the plant extractives where standard antibiotic discs of Nystatin (50 µg/disc) were used for comparison.

Source of Information: J. Life Earth Sci., Vol. 7: 61-65, 2012, ISSN 1990-4827

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The present extractives represent novel leads and so future studies may be undertaken for the development of a pharmacologically acceptable antimicrobial agent or class of agents. Our findings suggest the possibility of using the extractives in the treatment of various fungal infections and of discovering new bioactive compounds which can also be used for prophylactic treatments. Especially the bark of the plant has strong potential for the isolation of antioxidant and antimicrobial agents for functional pharmaceutical uses.

Knowledge Management: Journal