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Oviposition and Feeding Preference of Helicoverpa Armigera (hubner) on Vegetables

A. K. M. Z. Rahman,
Senior Scientific Officer
Entomology Division, BARI, Gazipur-1701

S. N. Alam
Chief Scientific Officer
Entomology Division, BARI, Gazipur-1701

M.A. Haque
Professor
Entomology Department, Bangladesh Agricultural University, Mymensingh,

Abstract/ Executive Summary:

Studies on host preference studies of Helicoverpa armigera (Hubner) were carried out at IPM lab of Entomology Division, Bangladesh Agricultural Research Institute (BARI), Gazipur, Bangladesh during October 2007 to August 2008. Five vegetables, viz., tomato, pea, country bean, French bean and brinjal were evaluated for oviposition and feeding preference of H. armigera under laboratory condition. The highest numbers of eggs (25.32) were laid on flowering French bean plants followed by flowering tomato plants (21.43), country bean (16.45) and flowering pea plants (9.38). While, brinjal flowering plants received no egg whatsoever. In feeding preference test, significantly the highest number of fruiting bodies were damaged in French bean (13.25) followed by country bean (9.50), pea (7.75) and tomato (4.00). No brinjal fruit was damaged by Helicoverpa armigira.

Keywords: Helicoverpa armigera, Oviposition ,vegetables, Brinjal, Tomato

Location: Gazipur

Start Date: 12-10-2007

End Date: 15-08-2008

Program Area: Pest Management

Commodity: Insects

Objectives:

To observe the oviposition and feeding preference of H. armigera on five selected vegetables by free choice test.

Methodology:

Tested vegetables: Five vegetables viz. country bean (Phaseolus vulgaris L.), pea (Pisum sativum L.), French bean (Phaseolus vulgaris L.), tomato (Solanum lycopersicum L.) and brinjal (Solanum melongena L.) were evaluated to observe the oviposition and feeding preference of H. armigera under laboratory condition.

Pot culture of vegetables: Earthen pots of diameter 35 cm were used to provide room for soil, roots and ensure good drainage and enough sun. Before planting the pots were filled with mixture of soil, organic fertilizer and compost @ 1:1:1 ratio. Tomato, pea, country bean, French bean and brinjal seedlings were grown separately from seeds in 300 pots and placed in a glasshouse maintained at 28 ± 2.5ºC and 75 ± 5% RH. Regular watering was done for proper growth and development of the plant. Each plant tightened with a split bamboo stick to avoid lodging. Different dates of planting were used to ensure continuous supply of flowering plants of all species at the time of the tests.

Experimental procedure for oviposition preference test: The oviposition preference of H. armigera was studied in the IPM lab of Entomology Division, Bangladesh Agricultural Research Institute (BARI), Gazipur, Bangladesh during October 2007 to August 2008 in a free choice test. Two flowering plants in a pot of each of the five vegetables, viz., tomato, pea, country bean, French bean and brinjal were positioned randomly relative to each other within a Perspex cage with wooden frames (2.5 x 1.5 x 2 m³). Plant positions were rotated daily to eliminate any positional effect. The cage was placed in a glasshouse and the plants were kept well apart from each other and potted plants were placed on different base height to keep their crowns at the same height in the cage to minimize any effects of plant height on oviposition. On the third day after emergence, the female moths were paired with a newly emerged male in a mating cage. On the fourth day one pair was released into the test cage at 5:00 pm. The following morning the eggs laid on each plant were counted and a new set of plants was provided with a new pair of moths until 20 pairs had been tested on 20 sets of plants. Because of the inherent variability in egg production among females, the proportion of eggs laid on each plant by each female moth was calculated relative to all eggs laid on all plants by each individual female. The eggs laid on the walls of the cage (<5% of all eggs) were not included in total egg laid. The experiment was laid out in completely randomized design (CRD) with three replications

Experimental procedure for feeding preference test: The feeding preference of H. armigera was studied in the IPM lab of Entomology Division, Bangladesh Agricultural Research Institute (BARI), Gazipur, Bangladesh during October 2007 to August 2008 in a free choice test. For this, twigs of selected vegetables containing fresh fruiting bodies (young fruits) were detached from the plant and their cut ends were wrapped in wet cotton. Each twig having at least three fruiting bodies was utilized. These fruits from five vegetables were kept together on a plastic tray (34cm x 22 cm x 4.5 cm) and kept in rearing case (61 cm X 61 cm X 76.5 cm). Now10 fifth instar larvae were released at the center of the tray within the cage. Fresh twigs containing young fruits of 5 vegetables were offered to the larvae daily for three days. The number of fruits in different fruiting bodies damaged by the larva after 24, 48 and 72 hours of initial release were counted. The feeding preference of the larva was determined on the basis of number of fruits damaged in tested vegetables. The higher the number of fruits damaged by the larva signified a greater preference by the H. armigera.

Source of Information: Bangladesh j. entomol. (2013) 23(1), 29-38 ISSN 1021-1004 A Scientific Journal of Bangladesh Entomological Society

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

It is concluded that flowering French bean plants received a significantly greater proportion of eggs than all the other plant species, flowering brinjal plants received no eggs whatsoever. The fifth instar larva did not prefer to feed on brinjal fruiting bodies as no fruiting body was found damaged during the study period.

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