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Bacterial Infestation in Different Fish at Rajshahi

H Khatun
Department of Fisheries, University of Rajshahi, Rajshahi-6205, Bangladesh

D A Khanom
Department of Fisheries, University of Rajshahi, Rajshahi-6205, Bangladesh

S N Jahan
Department of Fisheries, University of Rajshahi, Rajshahi-6205, Bangladesh

M D Hossain
Department of Fisheries, University of Rajshahi, Rajshahi-6205, Bangladesh

Abstract/ Executive Summary:

The present study was carried out from July 2005 to June 2007 and diseased freshwater fishes were collected from different water bodies and fish landing centre of three study areas, namely City Corporation area, Rajshahi; Paba upazila and Charghat upazila, Rajshahi. In the present study the load of bacteria in lesion, liver and kidney of H. molitrix showed considerable variation in different months during the study period. Monthly variation of bacterial load in body lesion varied from 3.17×104 CFU/g (July’05) to 2.13×10⁷ CFU/g (March’07), in liver it varied from 7.17×10³ CFU/g (July’05) to 5.13×10⁶ CFU/g (March’06) and in kidney it varied from 5.87×10³ CFU/g (July’05) to 6.33×10⁵ CFU/g (March’06). From two years observation, monthly variation of bacterial load in 3 sampling ponds were varied from 4.00×10⁶ CFU/ml (June’07) to 3.93×10⁷ CFU/ml (July’05) in case of pond-1. In pond-2 bacterial load varied from 1.27×10⁶ CFU/ml (May’07) to 5.33×10⁷ CFU/ml (September’06). In pond-3 the bacterial load varied from 2.10×10⁶ CFU/ml (June’07) to 2.10×10⁸ CFU/ml (July’06).

Keywords: Disease, Bacterial infestation, Bacterial load

Location: Rajshahi area

Start Date: 00-07-2005

End Date: 00-06-2007

Program Area: Pest Management

Commodity: Fish

Objectives:

The aim of the present study was to identify the common bacterial diseases in freshwater fishes. The loads in different organs and in the sampling ponds are to be assessed.

Methodology:

Procedure: Petridishes, test tube, glass pipettes, conical flask, tips, cotton physiological saline, Agar media were sterilized in an autoclave at 121°C for 20 minutes. Nutrient Agar media was made by 8.4 g agar and distilled water up to 300 ml and TSA media was made by 19 g agar and distilled water up to 500 ml. The conical flask was shaken with stopper for dilution of Agar and distilled water. Conical flasks containing agar were kept in an autoclave at 121°C for 20 minutes. Then the sterilized agar solution was poured separately (20 ml) in the petridishes from conical flask. Petridishes were closed and kept at room temperature for one hour. Collection of diseased fish: 18 types of fishes were collected from different fish landing centre, different water body and sampling ponds from July 2005 to June 2007. Bacterial isolates were collected from ulcer type lesion on body surface such as caudal, dorsal, ventral, lateral, head lesion and fin rot of different fish species viz. C. catla, L. rohita, C. mrigala, H. molitrix, C. idellus, C. carpio var. specularis, C. carpio var. communis, P. gonionotus, L. calbasu, P. ticto, C. punctatus, C. striatus, C. batrachus, H. fossilis, M. tengara, M. armatus, M. pancalus, A. testudineus. The samples were brought to the laboratory immediately after collection for bacteriological study. Bacteriological investigation: Tryptic Soy Agar (TSA) was used for culture of Aeromonas sp. Nutrient Agar was used for culture of Pseudomonas sp. And finally the identification of Aeromonas sp. and Pseudomonas sp. was made by Cowan and Steel’s Manual, for the identification of Medical Bacteria edited by Barrow and Felthem (1993) and confirmed with the help of Bergey’s Manual (Krieg and Holt, 1984). Sampling: Three specimens of H. molitrix were collected monthly from the sampling pond. Experiment was carried out by the method of Banu et al. (2001). Samples were collected from body lesion, liver and kidney of fish. Liver: The body surface of fish samples were disinfected with 70% ethyl alcohol. The abdomen of diseased fish was opened by aseptic dissection and then the liver was taken out carefully with the help of sterilized forceps. The liver samples after being weighed (0.1 g) individually in a sterile weighing boat was homogenized for preparation of the suspension in physiological saline. Thereafter, the suspension was diluted in physiological saline through 10 fold dilution for incubation on the culture media. Kidney: The kidney samples were prepared following the same procedure as done for the preparation of liver. Nutrient Agar was used for culture. Bacterial load in pond water: Water samples were collected from 3 sampling ponds, once a month during the study period. Water samples were collected in sterilized reagent bottles from the depth of 25-35 cm below the surface at the time from 10:00 am to 12:00 pm. These samples were 1ml diluted in sterile 9 ml physiological saline through 10 fold dilution for inoculation on the culture media (Nutrient Agar).

Source of Information: J. Sci. Foundation, 9(1&2): 77-84, June-December 2011, ISSN 1728-7855

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The present study revealed that the bacterial load in body lesion in H. molitrix varied from 3.17×10⁴ CFU/g (July 2005) to 2.23×10⁷ CFU/g (March 2007), in liver varied from 7.17×10³ CFU/g (July 2005) to 5.13×10⁶ CFU/g (March 2006) and in kidney varied from 5.87×10³ CFU/g (July 2005) to 6.3×10⁵ CFU/g (March 2006). In the present study the highest bacterial load in three sampling pond water was in the month of July 2006 and lowest was in the month of May, 2007. Bacterial load varied from 4.00×10⁶ CFU/ml (June 2007) to 3.93×10⁷ CFU/ml (July 2005) in sampling pond-1. In the sampling pond-2, bacterial load varied from 1.27×10⁶ CFU/ml (May 2007) to 5.43×10⁷ (September 2006). In the sampling pond-3 it was observed that bacterial load was varied from 2.10×10⁶ CFU/ml (June 2007) to 2.13×10⁸ CFU/ml (July 2006).

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