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Isolation and Identification of Bacterial Agents Causing Clinical Mastitis in Cattle in Mymensingh and Their Antibiogram Profile

Md. Tanvir Rahman*
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Md. Shafiqul Islam
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Mahmudul Hasan
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Abstract/ Executive Summary:

Mastitis is a serious problem for the dairy cattle in many countries of the world including Bangladesh. Among the microbial etiology bacteria plays a major role in the onset of the clinical form of the disease. Many of these bacteria are resistant to one or more antibiotics thus make the mastitis cases difficult to cure. In the present study Staphylococcus spp., Streptococcus spp., Bacillus spp. and E. coli were identified as the dominant bacterial species causing clinical mastitis in cattle in Mymensingh. Antibiogram study revealed chloramphenicol and erythromycin as the most effective antibiotic for the treatment of mastitis in these animals except those caused by Bacillus spp. and E. coli, respectively.

Keywords: Bovine clinical mastitis, Bacteria, Antibiogram, Mymensingh

Location: BAU Veterinary Clinics and in and around areas of BAU campus

Start Date: 00-07-2011

End Date: 00-10-2011

Program Area: Animal Health and Management

Commodity: Cattle

Objectives:

The present research work was carried out to isolate and identify dominant bacterial agents causing bovine clinical mastitis in Mymensingh and to determine the antibiotic susceptibility of isolated bacteria so that appropriate antibiotics could be suggested for their effective treatment.

Methodology:

A total of 16 milk samples were collected from the cases of bovine clinical mastitis during the period of July to October, 2011. The samples originated from BAU Veterinary Clinics and in and around areas of BAU campus. Samples were inoculated into various bacteriological media i.e., Blood agar, Nutrient agar, EMB agar, Nutrient broth to allow the growth of relevant bacteria. Isolation and identification of bacterial agents were performed based on their morphology, Gram’s staining, cultural and biochemical characteristics as described in Cheesbrough (2006). Finally, the antibiotic resistance pattern of selected bacterial agents was done by disc diffusion test according to the method of Bauer et al., (1966) using 11 randomly selected antibiotics (discussed later).

Source of Information: Microbes and Health, June 2013, 2(1): 19-21

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Results of our preset study suggests that chloramphenicol and erythromycin could be the most effective antibiotics to treat the cases of bovine clinical mastitis in Mymensingh except the cases caused by Bacillus spp., and E. coli, respectively.

Knowledge Management: Journal