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Winterfield 2512 G-61 Strain of Ibdv Vaccine (cevac® Ibd L) Showed Reduced Pathogenicity in Commercial Chickens

Mohammad Abu Sayed Khan*
Department of Pathology & Parasitology, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5202, Bangladesh

Md. Nazrul Islam
Department of Pathology & Parasitology, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5202, Bangladesh

S. M. Harun-ur-Rashid
Department of Pathology & Parasitology, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5202, Bangladesh

Mir Rowshan Akter
Department of Microbiology, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5202, Bangladesh

Abstract/ Executive Summary:

A live, freeze-dried commercial vaccine prepared from the “Winterfield 2512 G-61 strain” of infectious bursal disease virus (IBDV) was tested for it’s pathogenicity in commercial chickens. A total of 200 unvaccinated Cobb-500 chicks obtained commercial sources, raised in relative isolation from day old chick (DOC) were used in the experiment. A total of 3 chicks were randomly collected from the experimental flock on day 11 (D11), D13, D15, D17, D20 D23 and D26. The vaccine was administered through drinking water at D11 and the booster was also given on D17 through drinking water. All the sampled birds were euthanized for necropsy. The visible gross morbid lesions, bursa-body weight ratios and histomorphology including bursal lesion scores were recorded following necropsy and histopatholog. An infected flock was included in this study for comparison. Data of bursa-bodyweight ratios in relation to bursal lesion scores was analyzed statistically. No detectable gross lesions were found during necropsy and bursa-body weight ratios were 2.75±0.60, 2.71±0.39, 2.44±0.42, 3.39±0.13, 2.58±0.55, 2.15±0.16, 2.41±0.28 and 2.45±0.09 on D11, D13, D15, D17, D20, D23 and D26, respectively. Histopathological lesions were characterized as varying degrees of lymphoid depletion, and the bursal lesion scores were 0.67±0.33, 0.67±0.33, 2.00±0.58, 0.67±0.33, 1.0±0.00, 0.67±0.33 and 0.33±0.33 on D11, D13, D15, D17, D20 D23 and D26, respectively. The vaccine virus showed reduced levels of pathogenicity in broiler chickens. No disease outbreaks were noted in the vaccinated group, but significant changes were found in the naturally infected flock.

Keywords: Winterfield 2512 G-61 strain, IBDV vaccine, Pathogenicity

Location: Saidpur, Nilphamari, Bangladesh

Start Date: 00-09-2009

End Date: 00-02-2010

Program Area: Animal Health and Management

Commodity: Chicken

Objectives:

The present study has been carried out to determine the degree of pathogenicity of the Winterfield 2512 G- 61 strain of IBDV used in CEVAC® IBD L vaccine in commercial chickens.

Methodology:

Experimental chickens, research area and research period A total of 500 unvaccinated Cobb-500 Day Old Chicks (DOC) were selected from a commercial poultry farm in Saidpur, Nilphamari, Bangaldesh. Poultry rearing and vaccination was done at the source farm. Laboratory work was conducted at the Department of Pathology and Parasitology, Hajee Danesh Science and Technology University (HSTU), Bangladesh. The duration of the research work was six months from September 2009 to February 2010. Vaccine and Vaccination The Winterfield 2512 G-61 strain of IBDV, a live freeze-dried form of the isolate, was obtained directly from the ACI, Bangladesh Ltd., veterinary product seller and stored at 4ºC until used. The virus was propagated in embryonated chicken eggs obtained from specific-pathogen-free (SPF) flocks. Management of chickens The birds were reared in relative isolation. The room was thoroughly cleaned by sweeping and then washing with tap water using hose pipe connected with a tap. The room was disinfected with a household phenolic disinfectant (Phenyl) and fumigated before placing the DOC. Relatively optimum temperature in the brooder house was maintained using electric bulbs in required number and at required distances. Rice husk was the litter material which was placed at a 2-3 inch depth and was replaced as needed following wetting either by faeces, water or by both. For the first week white paper was placed in the brooder which was replaced regularly. Sampling and experimental design The birds were collected from the flock, and brought to the Department of Pathology and Parasitology for the necessary laboratory examination as per as experimental design outlined in Table 1. Necropsy and bursa-body weight (B/BW) ratio Necropsy was performed at the Pathology Laboratory of HSTU, Dinajpur as per standard procedure (Charlton, 2000). Each bird was weighed before euthanasia, and the visible gross morphological lesions were recorded. The Bursa of Fabricius was weighed and the average bursa-body weight ratio was determined as per the Tanimura et al., (1995) formula. Histopathological study and scoring of bursal lesions During necropsy, bursa of Fabricius was collected and preserved in 10% formalin for histopathological studies. The fixed tissue samples were further processed, embedded with paraffin, sectioned and stained with haematoxylin and eosin (H & E) as per standard method (Luna, 1968). Statistical analysis of bursal lesion scoring The raw data were recoded, entered and sorted using the MS Excel. The experimental data were calculated by the SPSS (Statistical Package for Social Sciences) (Version 11.5) for analysis. Mean differences were done by F test, t test and chi square test.

Source of Information: Microbes and Health, December 2012, 1(2): 58-61

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

This study revealed that administration of CEVAC® IBD L; a live freeze-dried vaccine produced by ACI, Bangladesh Limited from the “Winterfield 2512 G-61 strain” of infectious bursal disease virus was effective for protection against IBDV in chickens and the vaccine virus showed the reduced level of pathogenicity in broiler chickens. No detectable gross lesions were found during necropsy and bursa-body weight ratios determinations. Histopathological lesions were characterized as varying degrees of lymphoid depletion, and the bursal lesions scores. No outbreaks were noted in the vaccinated flock.

Knowledge Management: Journal