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Isolation and Characterization of Salmonella Serovars from Buffaloes in Mymensingh, Bangladesh

Nahid Rahman*
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Md. Shahidur Rahman Khan
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Md. Mansurul Amin
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Mahbubul Pratik Siddique
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Jayedul Hassan
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Kohinoor Parvin
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Abstract/ Executive Summary:

An investigation was carried out focusing the isolation and characterization of Salmonella serovars from buffaloes of some selected areas of Mymensingh district of Bangladesh. The objectives was to isolate and identify Salmonella serovars from diarrheic and apparently healthy buffaloes and to characterize the isolates by cultural and biochemical characteristics, serological tests and antibiotic sensitivity analysis. A total of 38 samples comprising rectal swabs and faeces were collected from 38 buffaloes originating from 3 selected areas of Mymensingh. Out of these 38 samples, 8 (20.63%) were found to be positive for Salmonella spp. All isolates fermented dextrose, maltose and mannitol with production of acid and gas but did not ferment sucrose and lactose. On the other hand, these isolates showed Indole and VogesProskaure test negative, Methyl-Red test positive. All these isolates subjected to rapid plate agglutination test with polyvalent “O” ( poly ?O’) and polyvalent “H” ( poly ?H’) antisera where positive agglutination were observed. All isolates were highly sensitive to ciprofloxacin, moderately sensitive to co-trimoxazole, gentamycin, tetracycline and less sensitive to erythromycin and resistant to furazolidone.

Keywords: Isolation, Characterization, Salmonella, Serovars, Buffalo, Mymensingh

Location: BAU dairy farm, Trishal and Boyra area, Mymensingh

Start Date:

End Date:

Program Area: Animal Health and Management

Commodity: Buffalo

Objectives:

Present study was designed to isolate and characterize Salmonella serovars using cultural, morphological, biochemical and serological examination to know the prevalence of Salmonellosis in buffaloes of Bangladesh as well as to reveal the antibiotic sensitivity and resistance pattern of the isolates.

Methodology:

Collection of samples: A total of 38 field samples comprising rectal swab from apparently healthy and diarrheic buffaloes, reared at BAU dairy farm, Trishal and Boyra area, Mymensingh, were collected under aseptic condition and carried out to the laboratory using Nutrient broth (NB). Isolation and characterization: The collected samples from apparently healthy and diarrheic buffaloes were inoculated to Nutrient agar (NA), SalmonellaShigella agar (SS), Brilliant green agar (BG), MacConkey agar (MC) and Eosin methylene blue agar (EMB). Petridishes were incubated at 37ºC for 24-48 hours and growing colonies were examined with Gram’s staining method (Merchant and Packer, 1967) and motility test with hanging drop slide (Cowan, 1974).Biochemical characterization of the isolates were performed with Sugar fermentation test, Methyl Red test(MR) and Voges-Proskaucer test (V-P) (Cheesbrough, 1985). Serological test: Salmonella agglutinating antiserum poly “O” and poly “H” (S & E reagents Lab, Bangkok, Thailand) was used to perform the serotyping of the isolated Salmonella spp. The macroscopic slide agglutination tests were performed. A single isolated colony from SS agar was emulsified with physiological saline solution. A single drop of thick bacterial suspension was placed on a glass slide and a drop of polyvalent antiserum was added. The slide was gently rotated to mix the fluid thoroughly. Antibiotic sensitivity test: Susceptibility of the isolated Salmonellae to different antibacterial agents was performed following the disc diffusion method (Bauer et al, 1966) to determine the drug sensitivity pattern. Sensitivity to antibiotic was mostly determined on Nutrient agar with tetracycline, gentamycin, co-trimoxazole, furozolidone, erythromycin, ciprofloxacin. The inhibitory effect of the antibacterials to the growth of the culture was recorded (Bauer et al., 1966).

Source of Information: Microbes and Health, December 2012, 1(2): 62-64

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The overall prevalence of Salmonella spp. was 21.05% from buffalo fecal samples as detected by cultural, morphological and biochemical examination and by slide agglutination test. This method may used for the rapid detection of Salmonella in field cases in buffaloes. Ciprofloxacin, co-trimoxazole, gentamycin appears to be the choice of drug from the list of antibacterial agents we tested. The antibacterial resistance observed here in the isolated Salmonellae might be due to indiscriminate use of these antibacterial agents in field condition in study areas and/or rapid chromosomal mutation and presence of specific plasmid DNA.

Knowledge Management: Journal