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Post-irradiation Ageing Effect on Cytological Characters of Crotalaria Saltiana

A.B.S. Shah
Professor Sultanul Alam Cytogenetics Laboratory, Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh

A. Naz
Department of Botany, Fulbari Govt. College, Fulbari, Dinajpur

M.M. Ud-Deen
Department of Crop Science & Technology, University of Rajshahi, Rajshahi-6205

G. Kabir
Department of Botany, University of Rajshahi, Rajshahi-6205

A. Akhter
Professor Sultanul Alam Cytogenetics Laboratory, Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh

Abstract/ Executive Summary:

Post-irradiation ageing effect on different cytological characters of Crotalaria saltiana were studied. Mitotic index followed no decreasing or increasing tendency based on the doses of gamma rays. Interphase chromosome volume was not found to increase with an increase of gamma rays. Percentage of pollen sterility was found to be lower in the lower doses and higher in the higher doses. Irregularities such as, formation of fragments, bridges, laggards, single and multiple chromatid bridges, unequal distribution and abnormal nuclear arrangement were recorded. This study reflects that irradiated seeds of Crotalaria saltiana showed no such dose wise effect and that might be due to ageing effect for long time.

Keywords: Post-Irradiation, Ageing Effect, Cytological Characters, Crotalaria Saltiana

Location: Cytogenetics Laboratory, Department of Botany, University of Rajshahi

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Pea

Objectives:

To evaluate the cytological changes in plants raised from thirty two years old irradiated (naturally) aged seeds of Crotalaria saltiana.

Methodology:

Thirty two years old irradiated (naturally) seeds of Crotalaria saltiana were used as plant material. Dry seeds of Crotalaria saltiana were irradiated with gamma rays by 5000 ci Co60 source at the rate of 10, 20, 30, 40, 50, 60, 70, 80, 90 and 100 Kr from the Atomic Energy Center, Dhaka. Seeds were obtained from Professor S.Alam Cytogenetics Laboratory, Department of Botany, Rajshahi University, Rajshahi for the present study. Part of the seeds was germinated on moist filter paper in petri dishes at room temperature in the laboratory and another part was germinated in pots. Germination percentages were recorded after seven days in laboratory and twelve day in pot. Root tips were collected and fixed into 1:3 acetic acid-alcohol for 48 hours and stored in 70% ethanol. Temporary slides were prepared by squash method using haematoxylin. Data were recorded on mitotic index, interphase chromosome volume (ICV) and various mitotic abnormalities from different stages. The nuclear volume (NV) was calculated using the formula for a sphere, NV = 4/3 πr³. The mean nuclear volume divided by the somatic chromosome number resulted the ICV. For meiotic study, young inflorescence obtained from dry seeds of Crotalaria saltiana which were irradiated with gamma rays by 5000 ci Co60 source at the rate of 10, 20, 30, 40, 50, 60, 70, 80, 90 and 100 Kr were collected in 1:3 aceto-alcohol. After 24-36 hours of fixation they were transferred to 70% ethanol. To collect data on meiotic abnormalities and pollen sterility, temporary slides were prepared from suitable anthers by acetocarmine smear technique. The findings obtained from the experiment were analyzed statistically MSTAT-C package program. The mean values recorded for all the treatments were compared by Duncun’s Multiple Range Test (DMRT).

Source of Information: Rajshahi University J. of Sci. 38, 87-95 (2010); ISSN 1681-0708

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

In the present findings post-irradiation ageing responded to different cytological characters of Crotalaria saltiana. The combined effect of post-irradiation and ageing indicated that with an increase of gamma rays there were no increase in mitotic index, ICV and percent of abnormality but showed various types of chromosomal irregularities.

Knowledge Management: Journal