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Microbiological Profile of Potato Samples Collected from Bangladesh Agricultural Research Institute (bari) and Notification of Anti-bacterial Traits

Mushfia Malek
Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh

Tasmina Rahman
Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh

Mrityunjoy Acharjee
Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh

Abstract/ Executive Summary:

Present study assessed the microbiological quality of different categories of consumable potatoes collected from Bangladesh Agricultural Research Institute (BARI), the largest multi-crop research institute of the country conducting research on a large number of crops. Five samples each from 15 categories of potato samples (Lara, Quincy, Cardinal, Esprit, Meridian, Lady Rossetta, Astrix, Soikat, Red potato, White potato, Russet potato, Yellow flesh potato, Sweet potato, Purple potato and Petiets potato) were subjected to microbiological analysis through conventional culture followed by the biochemical identification tests of the pathogens. A huge array of bacterial and fungal contamination was noticed within a range of (~108 cfu/g) in almost all samples studied. Among the bacterial pathogens, Escherichia coli and Staphylococcus spp. were found to be predominant. Study of antibiogram revealed that most of the isolates were resistant against the commonly used one or multiple antibiotics. Finally, all the samples were also examined for presence of any anti-microbial activity against different pathogenic bacterial species. Astrix potato samples exhibited antimicrobial activity against Shigella spp and the Russet potato samples exhibited the anti-bacterial activity against Staphylococcus spp.

Keywords: Potato; Microbiological quality; Drug-resistance; Antimicrobial activity; Consumer safety

Location: Department of Microbiology, Stamford University Bangladesh

Start Date:

End Date:

Program Area: Food Safety and Security

Commodity: Potato

Objectives:

1. To detect and quantify total viable bacteria, total and fecal coliforms, and specific pathogens in the collected potato samples; to demonstrate the drug-resistance traits of the pathogenic isolates; and finally to investigate the antimicrobial activity (if any) of the potato sample of current interest.

Methodology:

Study area, sampling and sample processing. Samples of 15 categories of healthy potato including Lara, Quincy, Cardinal, Esprit, Meridian, Lady Rossetta, Astrix, Soikat, Red potato, White potato, Russet potato, Yellow flesh potato, Sweet potato, Purple potato and Petiets potato were collected from Bangladesh Agricultural Research Institute (BARI) following standard protocol. Samples were quickly transported to the laboratory, and prior to microbiological assay, 10 g of each samples with 90 ml buffer peptone water (pH 7.2 ± 0.2) and the homogenized clear liquid was transferred into sterile airtight bottles. The homogenized samples were diluted up to 10-6 by following the standard methods of 10 fold dilution. Enumeration of total viable bacteria and fungi. An aliquot of 0.1 ml from the dilution 10-3 and 10-6 of each sample was spread onto nutrient agar (NA) plate (Hi-Media Laboratories Pvt. Ltd., India) for enumerating total viable bacteria (TVB) and on Sabouraud Dextrose agar (SDA) plate (Hi-Media Laboratories Pvt. Ltd., India) for the estimation of fungal load, by means of spread plate technique. Plates were incubated at 37⁰C and 25⁰C for 24-48 hours for bacterial and fungal enumerations, respectively. Estimation of total fecal coliform, Escherichia coli and Klebsiella spp. From the dilutions 10-3 and 10-6, 0.1 ml of each sample was spread onto the membrane fecal coliform (MFC) agar and MacConkey agar (Hi-Media Laboratories Pvt. Ltd., India) for the enumeration of total fecal coliform (TFC), and coliforms (i.e., Escherichia coli and Klebsiella spp.), followed by incubation at 44.5⁰C and 37⁰C for fecal coliform and coliforms, respectively for 24 hours. Estimation of Salmonella spp., Shigella spp., and Vibrio spp.. Enrichment was performed for Salmonella, Shigella and for Vibrio spp. in order to avoid the false negative results. After homogenization, 10 ml of samples were transferred into 90 ml of selenite cysteine broth (Oxoid Ltd., Basingstoke, Hampshire, England) and alkaline peptone water (APW) for the enrichment of Salmonella, Shigella, and Vibrio spp., respectively and incubated at 37⁰C for 4-6 hours. Samples were then diluted up to 10-6 and 0.1ml of samples from each of the 10-3 and 10-6 dilutions were spread onto Salmonella-Shigella (SS) agar (Hi-Media Laboratories Pvt. Ltd., India) and thiosulfate citrate bile salt sucrose (TCBS) agar (Hi-Media Laboratories Pvt. Ltd., India) for the isolation of Salmonella spp. and Shigella spp., and Vibrio spp., respectively. Plates were incubated at 37⁰C and the appearance of typical colonies (if any) was noticed within for 24-48 hours.

Source of Information: Stamford Journal of Microbiology, December 2013. Vol. 3, Issue 1, p. 21-25; ISSN: 2074-5346

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Interestingly in present study, antibacterial activity was found in the Astrix samples (9 mm) against Shigella spp. and Russet samples (11 mm) against Staphylococcus spp. However, the other samples showed no antimicrobial activity against any of the bacterial isolates. Therefore, the findings suggest that further study could be conducted focusing the self-protecting mechanism of the potato samples.

Knowledge Management: Journal