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An Assessment of Microbiological Quality of Some Commercially Packed and Fresh Fruit Juice Available in Dhaka City: a Comparative Study

Tasmina Rahman
Department of Microbiology, Stamford University Bangladesh

Rashed Noor
Department of Microbiology, Stamford University Bangladesh

Sabrina Hasan
Department of Microbiology, Stamford University Bangladesh

Abstract/ Executive Summary:

Fruit juices are regarded as the most preferred non-alcoholic beverage worldwide to all age groups. In general, microbial growth in fruit juice is restricted by using preservatives and also through other environmental factors during production. Still, the presence of some indicator organisms in fruit juice is responsible for causing food-borne diseases and even death. So, from public health point of view, it is quite important to know the microbiological quality of the fruit juices available in the market. Primary objective of this study was to assess the microbial quality of fresh and commercially packed available juices collected from different locations of Dhaka city. A total of six fresh juice and nine commercially packed juice samples were collected. Standard culture techniques were followed to assess total viable count (TVC), total staphylococcal count (TSC), total Bacillus count (TBC) and total fungal count (TFC) on different culture media. The TVC varied from the range of 102 to 105 cfu/ml with the highest of 2.4 x 105 cfu/ml. A large number of staphylococci and Bacillus was also found from several samples. Total coliform and fecal coliform was found in six and five (out of fifteen) samples, respectively. Among total coliforms, Klebsiella, Enterobacter along with E. coli were detected. From all the assessment it was determined that the microbial quality of commercially packed juice was fairer than that of fresh juice collected from local market.

Keywords: Microbiological Quality, Commercially Packed, Fresh Fruit Juice, Dhaka City

Location: Dhaka City

Start Date:

End Date:

Program Area: Quality and Nutrition

Commodity: Juice

Objectives:

The focus of this study was to compare the quality of fresh and commercially available fruit juices by assessing their microbial load and the presence of pathogenic bacteria.

Methodology:

Sample collection: Fifteen samples were collected, of which 6 fresh juice samples were collected from 5 different locations of Dhaka city and other 9 juice samples were commercially packed juices available in market. The table of collection sites are given below Enumeration of total bacteria. A total of 1 ml of sample was transferred with a sterile pipette to the tube containing 9ml of ringer solution to make 10-1 dilution. In such a way, dilution upto 10-6 was made. If in any sample countable number of colonies were detected at 10-6 dilution, further dilution of same item were prepared and tests were repeated using ringer solution. All colonies on the dishes were counted using a colony counter and following back calculating the actual numbers of bacteria. To enumerate the microbial load in the fruit juice sample, Plate Counting Agar (PCA) (Hi Media, India) for total viable bacterial count (TVC), Dextrose Tryptose Bromocresol Purple Agar (DTBPA) (Difco, USA) for total Bacillus count (TBC), Baired Parker Agar (BPA) (Difco, USA) for total Staphylococcal count (TSC), and Potato Dextrose Agar (PDA) (Hi Media, India) for total fungal count (TFC) were used. Isolation and identification of coliform: For presumptive identification of coliform bacteria, samples were inoculated onto MacConkey agar media. The isolates were then inoculated in Brilliant Green Lactose Bile (BGLB) broth followed by streaking on Eosine Methylene Blue (EMB) agar plate. Indole test, Methyl red test and Citrate utilization test were done to differentiate among groups of coliform bacteria. All media were prepared in the laboratory according to the manufacturer’s instruction. After inoculation of the samples, plates were incubated at 37°C and colonies were obseved after 24 hours.

Source of Information: Stamford Journal of Microbiology, July 2011. Vol. 1, Issue 1 ISSN: 2074-5346

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

From all the assessment it was determined that the microbial quality of commercially packed juice was fairer than that of fresh juice collected from local market.

Knowledge Management: Journal