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Optimisation of Ph, Temperature and Carbon Nitrogen Ratio for the Degradation of M-chlorophenol by Pseudomonas Putida Cp1

ANM Fakhruddin
Institute of Food & Radiation Biology (IFRB), Atomic Energy Research Establishment (AERE), Ganakbari, Savar, GPO Box 3787, Dhaka 1000, Bangladesh,

M Alamgir Hossain
Saidabad Water Treatment Plant, Dhaka WASA, Dhalpur, Saidabad, Dhaka 1204, Bangladesh

Abstract/ Executive Summary:

Aromatic pollutants like m-chlorophenol is toxic to the environment and chlorophenol containing a metachlorine are more persistent under aerobic conditions than compounds lacking a chlorine substituent in positions meta to hydroxyl group. Therefore, it should be removed effectively from the environment. In order to increase the degradative activity, the optimum conditions for m-chlorophenol degradation by Pseudomonas putida CP1, some physicochemical conditions like pH, temperature and carbon nitrogen ratio for the growth and degradation of most persistent monochlorophenol, m-chlorophenol by the organism was optimised. The pH optimum for m-chlorophenol degradation by the bacterium was between pH 6.5 and 7.0 and the temperature optimum was 30°C for removal activity. Carbon : nitrogen (C:N) ratio of 3:1 was found best for effective removal of chemical oxygen demand (COD) and m-chlorophenol by the bacterium.

Keywords: m-Chlorophenol degradation, Pseudomonas putida CP1, Chemical oxygen demand (COD)

Location: Institute of Food & Radiation Biology (IFRB), Atomic Energy Research Establishment (AERE), Ganakbari, Savar, GPO Box 3787, Dhaka

Start Date:

End Date:

Program Area: Food Safety and Security

Commodity: Fortified food

Objectives:

1. The aim of the present study was to optimise some physicochemical conditions such as pH, temperature and carbon nitrogen ratio for the growth and degradation of most persistent mono-chlorophenol, m-chlorophenol by Pseudomonas putida CP1.

Methodology:

P. putida CP1 was obtained from Dr. Favio Fava, University of Bologna, Italy. It was maintained on agar medium containing mchlorophenol (Aldrich Chemical Co, UK) at 4°C for and subcutured monthly. Inoculum was prepared by growing the bacterium overnight in nutrient broth, harvesting cells by centrifuged at 5,000 rpm for 10 min, washing twice with 0.01 M sodium phosphate buffer and re-suspending in distilled water. Culture suspension (5 ml) was used to inoculate 95 ml sterile minimal medium7 containing m-chlorophenol in 250-ml conical flasks. The minimal medium with an initial pH 7.0 contained 4.36 g/l K₂HPO₄, 3.45 g/l NaH₂PO₄, 1.26 g/l (NH₄)₂SO₄, 0.912 g/l MgSO₄.7H₂O and 1 ml/l trace solution. Trace salts solution contained (per 100 ml) 4.77 g CaCl₂.2H₂O, 0.37 g FeSO₄.7H₂O, 0.37 g CoCl₂.6H₂O, 0.10 g MnCl₂.4H₂O and 0.02 g Na2MoO₄.2H₂O. After inoculation, flasks were incubated in an orbital shaker at 150 rpm at 30°C. Samples were aseptically removed at regular intervals and analysed for growth, pH, chlorophenol removal, COD and for reducing sugar where appropriate.

Source of Information: Bangladesh J Microbiol, Volume 23, Number 2, December 2006, pp 159-161

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Carbon : nitrogen ratio (3:1) was found best for the complete removal of COD by P. putida CP1, which correlated the optimum C:N for bacteria. It could be concluded from the present study that P. putida CP1 could effectively remove COD and degrade m-chlorophenol in the presence of both carbon (fructose) and nitrogen (yeast extract) sources.

Knowledge Management: Journal