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Isolation and Identification of Ten Genera of Plant Parasitic Nematodes

M. M. Rahman
Department of Plant Pathology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh

I. H. Mian
Department of Plant Pathology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh

Abstract/ Executive Summary:

A study was undertaken to isolate and identify different genera of plant parasitic nematodes from soil and plant samples during July to October 2007 in the Laboratory of Plant Pathology at Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur, Bangladesh. Altogether 10 (Ten) nematodes belonging to 10 genera were isolated and identified from various soil and plant samples. For identification of plant parasitic nematodes, stylet and other anatomical structures such as Dorsal esophageal Gland Orifice (DGO), Esophageal lumen, Median bulb, Basal bulb, Intestine, Reproductive structures (vagina and its position, specula ) Tail types etc. were used as main criteria. Among 10 (ten) different identified genera of nematodes (Criconemoids sp., Hemicriconemoides sp., Tylenchus sp., Ditylenchus sp., Tylenchorhynchus sp., Hoplolaimus sp., Helicotylenchus sp., Pratylenchus sp., Meloidogyne sp., Radinaphelenchus sp.) maximum nematodes were under the family of Tylenchidae. The abundance numbers of plant parasitic nematodes were found as the Ditylenchus sp.(Ufra nematode) and Meloidogyne sp.(Root knot nematode) which were the serious plant pathogens causing ufra in rice and root knot in different crops respectively, so far recorded in Bangladesh.

Keywords: Isolation, Identification, Plant parasitic nematodes

Location: Laboratory of Plant Pathology at Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur

Start Date: 00-07-2007

End Date: 00-10-2007

Program Area: Pest Management

Commodity: Diseases

Objectives:

1. To isolate and identify different genera of plant parasitic nematodes from soil and plant samples.

Methodology:

Plant parasitic nematodes are generally isolated from the roots of plants they infect or from the soil surrounding the roots on which they feed. Suspected soil samples, roots and foliar plant parts were collected from crop fields of Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur, Bangladesh, Bangladesh Agricultural Research Institute (BARI) and Bangladesh Rice Research Institute (BRRI), Gazipur. A convenient method of collecting soil samples were 15-20 cm deep and the samples were with a portion of the root system and some of the distance foliage. The whole soil sample was about 1 L in volume. After collection the soil samples were carrying and stored in a polythene bag. Collected samples were placed following Bearmann Funnel Technique. The active nematodes moved through the tissue, leaving debris behind and concentrated at the bottom of the rubber tube. After 24 hours we collected suspected nematodes containing water funnel into Syracuse dish and observed it under dissecting micro- scope and plant parasitic nematodes were observed under compound microscope, noted different structures possessing stylet, and finally identified with the help of pictorial key to genera of plant parasitic nematodes.

Source of Information: Bangladesh J. Sci. Ind. Res. 45(3), 267-270, 2010

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

It may be concluded that active and alive nematodes can be isolated by Bearmann Funnel Technique. Plant parasitic nematodes must be posses axial stylet while non parasitic is lack of axial stylet. For identification of plant pathogenic nematodes stylet and other anatomical structures such as dorsal esophageal Gland Orifice (DGO), Esophageal lumen, Median bulb, Basal bulb, Intestine, Reproductive structures (vagina and its position, spicule), Tail types etc. were very useful. Among 10 (ten) identified genera of nematodes maximum nematodes were under the family of Tylenchidae. This study will be more helpful to the student and plant pathologists for nematode identification.

Knowledge Management: Journal