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Effect of BAU-biofungicide and Plant Extracts on Seedling Vigour of Maize

M. Debnath
Seed Pathology Center, Bangladesh Agricultural University, Mymensingh

A. Q. M. B. Rashid
Seed Pathology Center, Bangladesh Agricultural University, MymensinghFrom the present findings it is observed that the garlic extract, neem extract and the BAU-Biofugicide have the significant result in controlling seedborne fungal pathogen in maize and enhancing the seedling vigour. Among them, the BAU-Biofugicide has the strong potentiality for treating seeds of maize in controlling seed and seedling diseases. The seed treatment by BAU-Biofugicide may be widely practiced throughout the country

A. Sultana
Seed Pathology Center, Bangladesh Agricultural University, Mymensingh

Abstract/ Executive Summary:

An experiment was conducted in Seed Pathology Center, Bangladesh Agricultural University, Mymensingh to determine the efficacy of biocontrol means (BAU-Biofungicide, Garlic extract and Neem extract) and vigour index of seedling for controlling the seedborne fungal diseases of maize. In laboratory experiment, the prevalence of seed-borne fungi associated with maize cultivar cv. BARI Bhutta - 6 were investigated by blotter method. The identified fungi species were A. niger, A. flavus, Fusarium sp., P. oxalicum, C. lunata and R. stolonifer. The test materials were treated with BAU-Biofungicide @ 2.5% of seed weight, neem extract @ 1:2 w/v and 1:3 w/v and garlic extract @ 1:2 w/v and 1:3 w/v to control the pathogens. Significantly, the highest germination (87.%) and the lowest incidence of pathogen (4%) was recorded in BAU-Biofungicide treated seed The maize seeds treated by BAU- Biofungicide also show the higher vigour index (2856) followed by neem extract (1:2) treated seed (2621) after germination.

Keywords: Biofungicide, Germination, Maize, Pathogens

Location: Seed Pathology Center, Bangladesh Agricultural University, Mymensingh

Start Date:

End Date:

Program Area: Pest Management

Commodity: Maize

Objectives:

1. The present piece of work was undertaken to reduce the prevalence of the seedborne pathogen associated with the maize seeds and to enhance the seedling vigor.

Methodology:

Seed health test - The widely cultivated maize seed variety BARI Bhutta - 6 was collected from BADC of Mymensingh, Dinajpur, Madupur, Rangpur and Gazipur. The seed samples were tested by standard blotter method in Seed Pathology Center, Bangladesh Agricultural University, Mymensingh. Biofungicides - Biofungicides such as BAU-Biofungicide (Trichoderma based preparation), Garlic extract, Neem extract were collected from the Department of Plant Pathology, Bangladesh Agricultural University (BAU), Mymensingh. Seed treatment - One hundred seeds from each test materials were treated with BAU-Biofungicide @ 2.5% of seed weight, neem extract @ 1:2 w/v and 1:3 w/v and garlic extract @ 1:2 w/v and 1:3 w/v to control the seed borne pathogen. Experimental design and treatments - The experiment was designed following CRD having four replications and the following six treatments T₁= Seed treatment with garlic extract @ 1:2 w/v, T₂= Seed treatment with garlic extract @ 1:3 w/v, T₃= Seed treatment with neem extract @ 1:2 w/v, T₄ = Seed treatment with neem extract @ 1:3 w/v, T₅ = Seed treatment with BAU-Biofungicide, T₀ = Control Sowing of seeds and raising of seedling The seeds were sown @ 25 seeds per tray (1.5×1ft2) in three rows at 2-3 cm depth. Plants were watered whenever necessary. Recording of data - Data on germination failure and diseased seedling were recorded at 7, 10 and 20 days after sowing. For determining seedling vigour 3 seedlings per tray were carefully uprooted, cleaned with tap water. Then root length and shoot length were taken. Vigour index of seedling under different treatments were recorded as: Vigour index = (Mean root length + mean shoot length) × germination percentage. Analysis of data - The collected data were statistically analyzed and treatment means were compared following DMRT (Duncan’s Multiple Range Test).

Source of Information: J. Environ. Sci. & Natural Resources, 5(2): 59 - 61, 2012;ISSN 1999-7361J.

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

From the findings it is observed that the garlic extract, neem extract and the BAU-Biofugicide have the significant result in controlling seedborne fungal pathogen in maize and enhancing the seedling vigour. Among them, the BAU-Biofugicide has the strong potentiality for treating seeds of maize in controlling seed and seedling diseases. The seed treatment by BAU-Biofugicide may be widely practiced throughout the country.

Knowledge Management: Journal