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Effect of Ga3 on Biochemical Attributes and Yield of Summer Tomato

M. S. Rahman
Crop Physiology Division, Bangladesh Institute of Nuclear Agriculture, Mymensingh, Bangladesh

M. G. Mostofa
Crop Physiology Division, Bangladesh Institute of Nuclear Agriculture, Mymensingh, Bangladesh

M. A. Haque
Crop Physiology Division, Bangladesh Institute of Nuclear Agriculture, Mymensingh, Bangladesh

Abstract/ Executive Summary:

An experiment was carried out in pots at Bangladesh Institute of Nuclear Agriculture, Bangladesh to evaluate influence of different concentrations of GA3 on biochemical parameters at different growth stages in order to maximize yield of summer tomato var. Binatomato-2. The concentrations of GA₃ were 0, 25, 50, 75 and 100 ppm. They were applied at three stages, namely root soaking of seedlings before transplanting, vegetative and flowering stages. The experiment was laid out in a randomized complete block design with four replications. Results indicated that the highest chlorophyll and soluble protein contents were recorded when GA₃ was applied through root soaking followed by vegetative stage and the lowest was found at the flowering stage. In contrast, the highest nitrate reductase activity was observed when GA₃ was applied at the vegetative stage and the lowest activity was recorded at the flowering stage. The applications of 50-75 ppm GA₃ had significantly encouraged the bio-chemical parameters studied at 50 DAT. The amount of GA3 applied at different stages had significant influence on the yield and yield attributes of summer tomato. The highest plant height was recorded when 50 ppm of GA₃ was applied at the vegetative stage. While, the longest time to first fruit setting was required when the roots of the seedlings were soaked in 100 ppm GA₃ solution. The application of 50 ppm GA₃ by root soaking had significantly increased the number of flowers, fruits and fruit yield per plant but similar results were achieved when only 25 ppm GA₃ was applied at the flowering stage. The fruit yield of tomato per plant increased linearly with the increased number of flowers and fruits per plant.

Keywords: Summer Tomato, Gibberellic Acid, Chlorophyll, Soluble protein and Nitrate reductase activity

Location: Crop Botany Department, Bangladesh Agricultural University, Mymensingh

Start Date: 00-03-2012

End Date: 00-07-2012

Program Area: Crop-Soil-Water Management

Commodity: Tomato

Objectives:

To evaluate the performance of GA₃on biochemical parameters (chlorophyll, soluble protein and nitrate reductase activity) growth and yield attributes; and
To determine the optimum time and concentration of GA₃ application in order to maximize the yield of Binatomato-2.

Methodology:

A pot experiment was conducted with tomato var. Binatomato-2 at the experimental site of Crop Botany Department, Bangladesh Agricultural University, Mymensingh during March to July 2012. Five levels of GA₃ (Gibberellic Acid) viz. 0, 25, 50, 75 and 100 ppm were applied at (i) seedling stage by root soaking before transplanting (ii) vegetative stage [20 days after transplanting (DAT)] and (iii) flowering stage (40 DAT) by hand sprayer. The experiment was laid out in a randomized complete block design with four replications. Twelve kilograms of sun-dried sandy loam soil and cow dung, urea, TSP and MP @ 583.33, 2.9, 2.54 and 1.81 g/pot corresponding to 10 tons, 400, 350 and 250 kg/ha were applied in each pot (BARC, 2005). Before pot filling, full doses of TSP, MP and cow dung were thoroughly mixed with the soil. Half of the urea was applied during transplanting and the remaining half at vegetative stage. Intercultural operations were done as and when necessary. Data on yield and yield contributing characters were recorded at final harvest. Biochemical analyses viz. chlorophyll, soluble protein contents and nitrate reductase activities in leaves were estimated at 50 DAT following the methods of Arnon (1949), Lowry et al., (1951) and Stewart and Orebamjo (1979). Data were analyzed to find out the level of significance using MSTAT-C package programme (Russell, 1986) and significant differences among the treatments were adjudged by Duncan`s New Multiple Range Test (DMRT) at 5% level of significance (Gomez and Gomez, 1984).

Source of Information: Journal of Bioscience and Agriculture Research, Vol. 03 (02): 73-78, 2015, eISSN 2312-7945

Article Link (Online Journal): http://www.journalbinet.com/jbar-volume-03-issue-01.html

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Application of GA₃ at the rate of 50-75 ppm by root soaking and at vegetative stage increased biochemical parameters (chlorophyll, soluble protein and nitrate reductase activity) as well as increase the number of flowers, fruits and fruit yield per plant of Binatomato-2. It can be concluded that foliar application of 50 ppm Gibberelic acid on Binatomato-2 variety provide the best result in terms of biochemical attributes and yield.

Knowledge Management: Journal