U. Mayda
Dept. of Botany, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh
AFM Jamal Uddin
Dept. of Horticulture, Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh
H. Mehraj
The United Graduate School of Agricultural Sciences, Ehime University, 3-5-7 Tarami, Matsumaya, Ehime 790-8556, Japan
T. Taufique
Dept. of Horticulture, Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh
Rasheda Yasmin Shilpi
Dept. of Botany, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh
Arsenic, Rice, Pteris vittata, Inter-planting and trap plant
Department of Horticulture, Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh and Department of Botany, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh
Risk Management in Agriculture
Rice, Contamination of soil
Location and duration of the experiment: An experiment was conducted at Department of Horticulture of the Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh and at the Department of Botany of the Jahangirnagar University, Savar, Dhaka-1342, Bangladesh from October 2013 to April 2014.
Arsenic contaminated soil: Arsenic was applied in the form of Arsenic trioxide (As2O3) @ 50 ppm at 7 days before transplanting of the rice plant.
Treatments and design: P. vittata was inter-planted with rice into pot as trap to mitigate arsenic accumulation into rice plant. Experiment consisted three different density of the trap plants viz. P1: No P. vittata (Control); P2: four P. vittata plant per m2 and P3: eight P. vittata plant per m2 following completely randomized design with three replication. Trap plants were planted at the time of transplanting of rice plants.
Pot size: The pot size was 1.5 m in length, 0.5 m in width and 0.5 m in depth.
Data collection: Data were collected on plant height, number of tillers per plant, number of effective tillers per plant, panicle length, total number of grains per panicle, number of filled grains per panicle, number of unfilled grains per panicle, percentage of unfilled grains, 1000-grains weight, yield per plant, arsenic accumulation by rice grain, husk and straw, arsenic accumulation by P. vittata and reduction of arsenic accumulation in rice by P. vittata over control.
Chemical analysis for arsenic: Plant biomass was measured by using precision balance after drying. After growing, plants were collected and dried. After drying, samples were smashed by mortar and pastel machine. The arsenic analysis for was performed by using “Atomic Absorption Spectrometer”, where use of argon for carrier gas and arsenic was melted by 925ºC; was approved by ISO organization in Bangladesh Council of Scientific Research Institute (BCSIR), Dhaka, Bangladesh.
50 times dilution: 05 ml concentrated HCl was taken at 50 ml volumetric flasks for transferring arsenic into arsenic trioxide and a little bit of distilled water was added. Then 1ml solution was taken very carefully from each volumetric flask to avoid bubble and KI (01 gm) wash added in solution with 150 ml distilled water. After that 0.5 gm sample was taken in volumetric flask and mixed distilled water up to 50 ml and solution turned into yellow color. Another volumetric flask made blank solution, where contain only HCl, KI and distilled water for arsenic analysis.
1000 time dilution: 02 ml solution was taken into 500 volumetric flasks, mixed with distilled water up to 500 ml and shaken very carefully. Then, 05 ml solution was taken into 250 ml volumetric flask and mixed with distilled water up to 250 ml. Again, 05 ml solution from 250 ml solution was taken into 25 ml volumetric flask then 2.5 ml HCl and 2.5 ml KI was added and mixed distilled water, shaking was done very smoothly until turn it into yellow color. Standard solution arsenic was added with HCl 2, 5, 10, 15, 20 ppb respectively.
5000 time dilution: Similar to the 1000 time dilution. But 04 ml solution was taken in case of 2 ml. 05 ml HCl and KI was added into 5000 ml volumetric flask, solution was made into 25 ml and 05 ml was taken in flask.
Statistical analysis: Data were statistically analyzed using MSTAT-C computer package programme. Difference between treatments was assessed by Least Significance Difference (LSD) test at 5% level of significance.
Journal of Bioscience and Agriculture Research, Vol. 04, Issue 02: 60-66, 2015.
Journal