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Effect of Growth Regulators on Germination and Vigor of Lentil Seeds

M. A. I. Pulok
Dept. of Seed Technology,Sher-e-Bangla Agricultural University, Dhaka, Bangladesh

M. S. Ali
Dept. of Agronomy,Sher-e-Bangla Agricultural University, Dhaka, Bangladesh

R. Chakraborty
Dept. of Agronomy,Sher-e-Bangla Agricultural University, Dhaka, Bangladesh

M. N. Haque
Dept. of Agronomy,Sher-e-Bangla Agricultural University, Dhaka, Bangladesh

M. M. Rahman
Dept. of Agronomy,Sher-e-Bangla Agricultural University, Dhaka, Bangladesh

Abstract/ Executive Summary:

An experiment was carried out to evaluate the effect of growth regulators viz., Gibberellin (GA₃), lemon juice and tamarind leaf extract on germination and vigor of lentil seeds. The experiment was conducted with BARI Masur-4 cultivar, and three growth regulators: gibberellin, lemon juice and tamarind leaf extract were used. Three concentrations of gibberellins (10, 20 and 30 ppm), three concentrations of lemon juice (2, 4 and 6 %) and three concentrations of tamarind leaf extract (0.05, 0.1 and 0.15%) with two soaking time duration: 6 and 12 hours and control were used. Gibberellin (GA₃) improved germination percentage, seedlings length, dry matter, germination index and vigor index of lentil. Lemon juice enhanced germination percentage, seedling length, vigor index and germination index in lentil. Tamarind leaf extract increased the germination percentage, vigor index and speed of germination in lentil. In comparison with plant growth regulators, length and weight was found to be positively correlated and in most cases relationships were significant.

Keywords: Lentil, Growth regulators, Germination and Vigor

Location: Seed Quality Laboratory, Institute of Seed Technology, Sher-e-Bangla Agricultural University, Dhaka-1207

Start Date: 00-11-2011

End Date: 00-03-2012

Program Area: Crop-Soil-Water Management

Commodity: Lentil

Objectives:

(i) To increase the seed germination of lentil seeds under soaking in varying concentrations of different natural and synthetic growth regulators,

(ii) To evaluate vigor of different lentil seeds under soaking in varying concentrations of different natural and synthetic growth regulators and

(iii) To evaluate seedling parameters of lentil under soaking in varying concentrations of different natural and synthetic growth regulators.

Methodology:

The research work related to determine the effect of organic and inorganic growth regulators on germination and vigor which was conducted in the Seed Quality Laboratory, Institute of Seed Technology, Sher-e-Bangla Agricultural University, Dhaka-1207 during November 2011 to March 2012. Furthermore this experiment was done within the project entitled ‘Study on the Agronomic Seed Health and Quality Testing and Improvement” by Japan Society for Promotion Science (JSCS) (Yamagata University) and University Grants Commission of Bangladesh.
The experimental area was situated at 23°77'N latitude and 90°33'E longitude at an altitude of 8.6 meter above the sea level. The genetically pure and physically healthy BARI lentil-4 seeds were collected from of Bangladesh Agricultural Research Institute (BARI), Gazipur. 13 cm diameter sized pots wereu sed to perform the study. A two factor experiment was laid out in a randomized complete block design (factorial) with three replications. Seeds were soaked in different concentrations of three growth regulators: Lemon juice (Citrus limon), Tamarind leaf extract (Tamarindus indica) and Gibberellin (GA₃).
This experiment consisted of 21 treatments, which are as follows, S₀= Without soaking, S₆ = Soaking in distilled water for 6 hours, S₁₂ = Soaking in distilled water for 12 hours, L₁H₆ = Soaking in lemon juice 2% for 6 hours, L₁H₁₂ = Soaking in lemon juice 2% for 12 hours, L₂H₆ = Soaking in lemon juice 4% for 6 hours, L₂H₁₂ = Soaking in lemon juice 4% for 12 hours, L₃H₆ = Soaking in lemon juice 6% for 6 hours, L₃H₁₂ = Soaking in lemon juice 6% for 12 hours, T₁H₆ = Soaking in tamarind leaf extract 0.05% for 6 hours, T₁H₁₂= Soaking in tamarind leaf extract 0.05% for 12 hours, T₂H₆ = Soaking in tamarind leaf extract 0.1% for 6 hours, T₂H₁₂ = Soaking in tamarind leaf extract 0.1% for 12 hours, T₃H₆= Soaking in tamarind leaf extract 0.15% for 6 hours, T₃H₁₂ = Soaking in tamarind leaf extract 0.15% for 12 hours, G₁H₆ = Soaking in gibberellin 10 ppm for 6 hours, G₁H₁₂ = Soaking in gibberellin 10 ppm for 12 hours, G₂H₆ = Soaking in gibberellin 20 ppm for 6 hours, G₂H₁₂ = Soaking in gibberellin 20 ppm for 12 hours, G₃H₆ = Soaking in gibberellin 30 ppm for 6 hours and G₃H₁₂ = Soaking in gibberellin 30 ppm for 12 hours.
100 seeds in each pot were placed. Performance of natural and synthetic growth regulators was evaluated on the basis of laboratory germination test by collecting different germination parameters e.g. germination percentage (%) and seedling growth parameters i.e. shoot length, shoot fresh and dry weight, vigor index, speed of germination and germination index. For recording length, 20 seedlings in each pot were selected randomly. For assessing fresh and dry weight, seedlings of whole pot were considered. Germination meant when either cotyledons or plumule emerged above the sand upper surface. For dry weight determination, the seedlings were removed and dried in an electric oven at 70°C temperature for 48 hours.
The data obtained for different studied characters were statistically analyzed following the analysis of variance techniques by using MSTAT-C computer package programme. The significant differences among the treatments means were compared by Least Significant Difference (LSD) at 5% level of probability.

Source of Information: Journal of Bioscience and Agriculture Research, Vol. 03 (01): 08-14, 2015, Online ISSN 2312-7945

Article Link (Online Journal): http://www.journalbinet.com/jbar-volume-03-issue-01.html

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

It may be concluded that performances of lentil seeds, in terms of different germination and vigor characteristics, were better when growth regulator’s components used, and when seeds were soaked by low or medium concentrations among tested all growth regulators combinations. But higher concentration of different growth regulators with defined time schedule may make an insignificant effect in many of those seed germination and vigor parameters, as evidenced in the case of lemon juice of this experiment.

Knowledge Management: Journal