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Genetics of Purple Pigment Development in Different Plant Organs of Mungbean

M. I. Akhtar
Principal Scientific Officer
TCRC, BARI, Joydebpur, Gazipur 1701, Bangladesh

A. Quasem
Director
TCRC, BARI, Joydebpur, Gazipur 1701, Bangladesh

S. K. Bhadra
Professor
Department of Genetics and Plant Breeding, Chittagong University

Abstract/ Executive Summary:

Development of purple pigment in 13 different plant organs, namely hypocotyl, cotyledon, epicotyl, stem, petiole, leaf rachis, leaflet base, leaf veins, peduncle, pedicel, calyx, standard and unripe pod suture of mungbean was under the control of six different loci, each having two alleles. Gene symbols P1, P2, P3, P4, P5 and P6 were proposed for the genes regulating the development of purple pigment in hypocotyl, (cotyledon+epicotyl), stem, (petiole+leaf rachis+leaflet base), leaf veins and (peduncle+pedicel+calyx+standard+unripe pod suture), respectively. The symbols proposed for their respective alternate alleles, which regulated the development of green pigment were P1, P2, P3, P4, P5 and P6. Among the six loci P1 was linked to P2 and P4 to P5. Linkage analysis revealed that the average cross over percentage between the loci P1 and P2 was 27.77 and that between P4 and P5 was 29.25.

Keywords: Genetics, Purple pigment development, Plant organs, Mungbean

Location: TCRC, BARI, Joydebpur, Gazipur

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Mungbean

Objectives:

To study the genetics of this pigment development in 13 different organs of mungbean viz., hypocotyl, cotyledon, epicotyl, stem, petiole, leaf rachis, leaflet base, leaf veins, peduncle, pedicel, calyx, standard and unripe pod suture.

Methodology:

The F₂ and backross segregation of 8 different crosses (CUM 81022×CUM 81035, CUM 81141×CUM 82201, CUM 82607×CUM 81141, CUM 81022×CUM 82201, CUM 82097×CUM 82201, CUM 81035×CUM 82201, CUM 82607×CUM 82201 and CUM 82607×CUM 81245) involving seven cultivars were studied for the genetic analysis of the development of purple pigment in 13 different organs of mungbean plant. The seven parental cultivars included differed from one another in respect of pigmentation in different plant organs. Crossing was done between the selected parents to get F₁hybrids including reciprocals. Since there was no reciprocal difference in the F₁ generation, the F₂ seeds of each cross combination were bulked. Backrosses were made between F₁ and their respective parents to get B₁ and B₂ seeds. Parental, F₁_,F₂, B₁ and B₂ generations were thoroughly studied for this pigment character. The frequency of plants having purple and green pigments in different organs was recorded. Genetic interpretations were made on the basis of F₂ backcross segregations. In the case of the trigenic crosses showing a discrepancy from the expected Mendelian trigenic ratio, the F₂ and backcross segregation data were further analysed at digenic level to identify the causes of the discrepancies. In the case of linked genes, recombination percentage was computed from the F₂ and backcross data by maximum likelihood method. Combined value of recombination fraction between two linked loci was computed from the F₂ and backcross data for each of the five crosses displaying linkage. Thereafter, the average recombination percentage was estimated for the linked genes.

Source of Information: BANGLADESH JOURNAL OF AGRICULTURAL RESEARCH, VOL-24(2), PP.319-333, JUNE 1999, ISSN 0258-7122.

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

From the present study, it is revealed that five different loci were involved in the development of pigment in these eight different organs. Among the six loci P1 was linked to P2 and P4 to P5. Linkage analysis revealed that the average cross over percentage between the loci P1 and P2 was 27.77 and that between P4 and P5 was 29.25. However, the current findings on the genetics of pigmentation in different plant organs of mungbean would be helpful in future genetic and breeding research programmes.

Knowledge Management: Journal