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Physiology of Nacl Tolerance in Calli of Vigna Radiata (l) Wilczek

S. M. lmamul Huq
Department of Soil Science, University of Dhaka, Dhaka-1000, Bangladesh.

S. Hadiuzzaman
Department of Botany, University of Dhaka. Dhaka-1000, Bangladesh.

M. A. Kafi Miah
Department of Soil Science. University of Dhaka, Dhaka-1000, Bangladesh.

Abstract/ Executive Summary:

Calli of two strains of Vigna radial a. CUM 82007 and CUM 82037 were cultured on the callus proliferation medium (MS + 2.0 mg 1^-IBA + 0.2 mg 1^-1 NAA) In presence of NaCl concentrations ranging from 0 to 150 mM. Calli of CUM 82007 exhibited salt tolerance upto 100 mM NaCl ext while CUM 82037 was susceptible to salinity at this concentration. Presence of NaCI in the medium affected various growth parameters differently of the Calli of the two strains. Correlation coefficients between external and internal Na + concentrations with various growth parameters as well as soluble sugars and proline contents revealed that proline is a better stress marker than sugars and It accumulates In the cells possibly to withstand situation of salinity stress. However, the salt tolerant strain accumulates relatively less proline than susceptible one. Free sugars appear to have a more important role In salt tolerance of the legumes.

Keywords: Salt tolerance, Compatible solute, Osmotic adjustment, NaCl ext. Endogenous Na+, Proline, Free sugars.

Location: Department of Soil Science, University of Dhaka, Dhaka-1000, Bangladesh.

Start Date:

End Date:

Program Area: Crop-Soil-Water Management

Commodity: Contamination of soil, Mungbean

Objectives:

To determine the Physiology of NaCl Tolerance in Calli of Vigna Radiata (L) Wilczek

Methodology:

Seeds of Vigna radiata (L) Wilczek. CUM-82007 and CUM-82037 were aseptically germinated on sugar (2%) and agar (1%) media. Calli were initiated from hypocotyl explants of 5 days old seedlings in MS medium supplemented with seven different hormonal combinations and were maintained on MS + 2.0 mg 1-1 BA + 0.2 mg 1-1 NAA. Calli obtained from hypocotyl explants after three passages of growth were transferred to the callus proliferating g medium supplemented with different concentrations of NaCI (0. 10. 25. 50. 100 and 150 mM). Samples of calli were collected carefully from each culture tube so that agar/medium did not adhere to it. The fresh and dry weights of each sample were determined and the water contents calculated. The results presented are the averages of three individual replicates of three samples in each. Plant materials were extracted with cold 80% ethanol. The suspension was evaporated in a rotary evaporator and the residue was dissolved in deionized water. This extract was used for the determination of Na and K by flame photometry, proline and soluble sugars by spectropholometric analyses following the methods respectively. Total soluble sugars are expressed in terms of glucose. The results are presented in terms of dry matter, m mol^-3 or mM as and when needed. Simple correlations were calculated on the results to study the relationship between external Na+. cellular Na+ and other parameters to determine the relationships of NaCl acclimation of the two strains.

Source of Information: Bangladesh J. Bot. 22 (1): 29-36, 1993 (June)

Article Link (Online Journal): http://agris.fao.org/aos/records/BD9325420

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The present observation further conforms the doubt put forward by these authors (1985) about the possible role of proline in the cytoplasmic adjustment in legumes. So far the soluble sugars are concerned these solutes appear to play a more important role In osmotic adjustments in legumes under salinity stress. The present observations lead to such a conclusion. A possible Involvement of Na⁺ in cytoplasmic adjustment of legumes when they acclimate to NaCl salinity is there too that 4 .4% of the tissues total Na⁺ can be present in the cytoplasm of cotton leaf cells. Proline accumulation in the calli of the two strains of V. radiata acclimated to salinity could be either symptomatic of a salt induced water deficit or a temporary deviation of the metabolic pathway of NH₄ assimilation at the level of glutamic acid.

Knowledge Management: Journal