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In Vitro Growth and Microbulb Production of Garlic with Bap and Naa

M. F. Mondal
Department of Horticulture, Bangladesh Agricultural University, Mymensingh.

M. G. Rabbani
Department of Horticulture, Bangladesh Agricultural University, Mymensingh.

M. S. Alam
Department of Horticulture, Bangladesh Agricultural University, Mymensingh.

Abstract/ Executive Summary:

Effects of growth regulators benzylaminopurine (BAP) and naphthalene acetic acid (NAA) each at three concentrations (0, 1 and 2 mg/l) were assessed on shoot multiplication and microbulb production of garlic through tissue culture. Significant variations were observed among the treatments in respect of all the parameters studied. BAP at 2 mg/l was found to be the best for rapid shoot multiplication and clove production per microbulb followed by 1 mg/I BAP. Growth and development of roots and the microbulbs were the best at 2 mg/l NAA followed by 1 mg/l NAA.

Keywords: BAP, NAA, Microbulb, Allium

Location: Horticulture Farm, Bangladesh Agricultural University, Mymensingh

Start Date:

End Date:

Program Area: Crop-Soil-Water Management

Commodity: Garlic

Objectives:

To assess the effects of BAP and NAA on multiplication and microbulb production of garlic through tissue culture .

Methodology:

Local variety of garlic collected from Horticulture Farm of Bangladesh Agricultural University, Mymensingh was used in the present study. Garlic bulbs were separated into cloves and the outer, dry, papery bulb scales were removed. The cloves were then gently washed under running water, placed in 70% ethyl alcohol containing few drops of Tween 20 for one minute and rinsed three times with sterile distilled water to remove ethyl alcohol. After this pretreatment, the cloves were disinfected in 0.1 % mercuric chloride solution containing a few drops of Tween 20 and agitated for 1 minute and rinsed three times with sterile distilled water to remove HgCl₂ from the explant. Sterilization of explants was followed by several rinses with sterile distilled water. Murashige and Skoog medium (4.71g) of "Imperial Brand" was used as culture medium. The medium was sterilized in an autoclave at a temperature of 121°C for 20 minutes at 1.16 kg/cm² pressure. The shoot tip explants at about 4mm long were inoculated to each culture vessel. The explants were placed on MS media. The cultures were maintained in a growth room at 25±2°C illuminated with cool light from 1.83 m 'Colour 84' fluorescent tubes. The room was illuminated for 16 hr daily with a light intensity of 1500 lux. Five treatments viz., control, 1 mg/l each of BAP and NAA and 2 mg/l each of BAP and NAA were used in the study. The experiment was laid out in completely randomized design with three replications. The data for the characters under study were statistically analyzed wherever applicable. The analysis of variances were performed by F-variance test. The significance of differences between pairs of means was evaluated by Least Significance Difference (LSD) test.

Source of Information: Bangladesh Hort.,26(1&2):45-48,1998; ISSN:0379-4288.

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The medium with 1 mg/l NAA produced the highest percentage of microbulb (92%) and the lowest (20%) was observed in the medium without growth regulator.The maximum number of cloves per microbulb (5.50) was obtained at 2 mg/l BAP while the lowest (1.10) was in the control treatment. The present study recommended 2 mg/I BAP for rapid shoot multiplication and clove production and 2 mg/I NAA for growth and development of roots and microbulbs of garlic.

Knowledge Management: Journal