Dr. Md. Bahanur Rahman
Associate Professor
Department of Pathology, BAU, Mymensingh-2202
M. H. Rahman
Department of Pathology, BAU, Mymensingh-2202
A. Islam
Department of Pathology, BAU, Mymensingh-2202
The aim of this study was to investigate the pathological conditions in ovary and reproductive tract of does at slaughter and isolation and identification of chlamydiae from the reproductive tract of goats. A total of 51 female genital tract samples together with ovaries from does > 1 and half year old were randomly collected from the slaughtered goats at Mymensingh district abattoir from August 2004 to March 2005. After collection, representative tissue samples were preserved in 10% neutral buffered formalin and swabs were collected in sterilized tubes containing nutrient broth. Tissues were processed, embedded in paraffin, sectioned and stained using standard histopathological techniques. Swabs were subjected for routine bacteriological examinations. Immunohistochemical examination was also performed on paraffin sections using a genus specific monoclonal anti-chlamydial antibody and DAKO techmate immunperoxidase kit. Know chlamydial isolates were propagated in chicken embryo fibroblast culture to standardize the CPE. Samples were inoculated in chicken embryo fibroblast cell culture for the isolation of chlamydiae. Among these 51 does, 21 did not show lesion in the ovaries and in the uterine tract, uterus of 3 does contained early embryos and 27 does showed lesions either in ovaries or somewhere in the reproductive tract. The highest pathological conditions occurred in the uterus 37.7% (endometritis: 28%, endometritis plus salpingitis: 3.9%, endometritid plus cevicitis: 3.9% and adenomyosis:1.9%) followed by the ovaries (Follicular cyst: 3.9%, par ovarian cyst: 3.9%, ophoritis: 1.9%, granulose theca cell tumor: 5.88). Staphylococcus, Bacillus and Diplococcus were isolated from the uterine swab of 25 goats. Chlamydia could not be detected in any of the sections immunohistochemically. Chlamydiae were not isolated from any of the samples. Results showed that a large number of female goats slaughtered at Mymensingh district abattoir affected with reproductive abnormalities. Granulose theca cell tumor is the first report in the ovary of Black Bengal goats.
Identification of Chlamydiae, Caprine abortions, Slaughter, Black Bengal Goats
Mymensingh district.
Pest Management
1. Isolation of chlamydiae from the reproductive tract of infertile goats
2. Isolation of chlamydiae from the cases of caprine abortions.
3. Typing of Bangladesh isolates using species specific monoclonal antibodies/PCR.
4. Histopathology of chlamydial infection.
a. Materials / Samples collection.
b. Design of experiments: Described in materials.
c. Procedures to be followed in achieving objectives.
Cytological and microbiological study, ii) Isolation of chlamydaie iii) Histopathology iv) Immunohistochemistry/PCR
Bangladesh Agricultural University, Research Progress. Volume-17,
Proceedings of the workshop of BAU Research Progress, 03-04 December 2006
150000
Isolation of chlamydiae from the cases of caprine abortions, Typing of Bangladesh isolates using species specific monoclonal antibodies/PCR and Histopathology of chlamydial infection were identified from any of the smaples. A large number of female goats slaughtered at Mymensingh district abattoir affected with reproductive abnormalities. Granulose theca cell tumor is the first report in the ovary of Black Bengal goats. Staphylococcus, Bacillus and Diplococcus were isolated from the uterine swab goats. Chlamydia could be detected in any of the sections immunohistochemically.
Report/Proceedings