Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Role of Platelet Rich Plasma Gel in the Wound Healing of Black Bengal Goat

Raihana Nasrin Ferdousy
Department of surgery and obstetrics, Bangladesh Agricultural University, Mymensingh, Bangladesh.

Md. Abu Hadi Nur Ali Khan
Departmeny of pathologyrtment of pathology, Bangladesh Agricultural University, Bangladesh.

Sattwikesh Paul
Department of surgery and obstetrics, Bangladesh Agricultural University, Bangladesh

Md. Mizanur Rahman
Department of surgery and obstetrics, Bangladesh Agricultural University, Bangladesh

Abstract/ Executive Summary:

The study was conducted to evaluate the efficiency of platelet rich plasma (PRP) gel in the healing of skin wound. This experiment was carried out to exploit the therapeutic effects of PRP gel on healing process. A. A total of 72 surgical wounds were madein 12 goats bearing weight 15-20 kg with the age 1-3 years. Goats were divided into four groups with three animals in each group. Wounds of four groups were treated with homogenous PRP gel, heterogeneous PRP gel, sulfanilamide powder and tincture benzoinCo. or benzoin seal (control). The wounds were 3 cm length and 0.5 cm depth sutured with cross mattress using nylon. Follow-up observation was recorded from day 1 to day 30 postoperatively. Some morphological characters such as swelling area of wound, elevation of sutured line from the skin surface, width of sutured area and contraction length between 7 to 15 days were recorded to determine the healing process of the wounds. Besides morphological study, histopathological studies were also performed on day 1, day 2, day 3, day 5 and day 15 to evaluate the healing process. Homogenous PRP gel showed admirable results in the healing of the wounds produced in goat. All wounds treated with homogenous PRP gel showed on inflammation with dry sutured area and complete healing with massive hair follicular growth within 15-21 days. On histopathological study, demonstrated epithelial tissues with a normal morphology. The dermis showed flabby connective tissue with organized interconnecting collagen fibers running parallel to each other and new hair follicular growth in healed area within 15 days, when sounds were treated with homogenous PRP gel. This study could help to consider natural biomaterial product specially PRP gel homogenous for a good healing of skin wounds with minimum complications. Further studies are necessary for the molecular investigation of healing process and proper establishment of commercially available of PRP gel and easiest finding the way of it's application.

Keywords: Black Bengal goats, Platelet rich plasma gel, Skin wound, Healing

Location: Department of Surgery and Obstetrics, at the Bangladesh Agricultural University (BAU), Mymensingh, Bangladesh.

Start Date: 01-11-2012

End Date: 31-07-2013

Program Area: Animal Health and Management

Commodity: Diseases

Objectives:

To find out the easiest way of PRP gel formulation and exploit the therapeutic effects of PRP gel in skin sound of Back Bengal goat.

Methodology:

A number of experiments were performed in black Bengal goat to find out the effect platelets rich plasma (PRP) gel in healing of skin wounds. The proposed research works were conducted at the Department of Surgery and Obstetrics, at the Bangladesh Agricultural University (BAU), Mymensingh. The duration of the study was from November, 2012 to July, 2013. Twelve apparently healthy black Bengal goats were used for the experiment. The age of goats ranged from 1-3 years and body weight ranged from 15-20 kg. All the goats were maintained hygienically in animal shed of the Department of Surgery and Obstetrics. All of the experimental goats were dewormed with levamisol hydrochloride and triclabendazole and vaccinated with Tetavex. The blood was preliminary stored in Falcon tubes containing 3.8% sodium citrate anticoagulant. The tubes were centrifuged at 3000 rpm for 10 min promoting the separation of the plasma from red blood cells. After that, 2 ml of plasma was removed from the superior part of each tube and was taken in another tube called tube A. This part was used to obtain the autogenous thrombin. 600 μl of 10% calcium gluconate was added in tube A and incubated at at 37 ºC for 15 min. Total volume of plasma in tube A is thrombin rich substrate. A total of 72 skin wounds were created on the skin of twelve goats. Goats were divided into four groups with three animals in each group. All works have been repeated for three times in each group. Wounds created in each goat was 3cm length and 0.5cm depth. One of four treatments was randomly assigned to each wound in each animal: (1) application of homogenous PRP gel, (2) application of heterogenous PRP gel, (3) application of sulphonilamide powder and (4) application of benzoin Co. in the subcutaneous tissue and the wound were closed in a cross mattress pattern with 2.0 nylon. Repeated wounds were made at least after 15 days in another animal. For histopathological study, tissue samples were collected from treatment groups as well as control at day 1 (D₁), Day 2 (D₂), day 3 (D₃), day 5 (D₅) and day 15 (D₁₅) respectively. The biopsies (1.5cm X 1cm) were collected from wound area of each experimental animal. The biopsy sites were cleaned with saline solution and gauze. The sample were fixed in 10% PBS buffered formalin solution more than seven days for histopathology. Statistical analysis were done by using utilized SPSS statistical software. All data were presented as mean±SEM. Total 18 data for each group, have been repeated for three times. To compare data among groups, one way ANOVA (Analysis of Variance) factor analysis was performed. For all statistical tests, p-value was 0.05.

Source of Information: IOSR Journal of Agriculture and Veterinary Science (IOSR-JAVS); e-ISSN: 2319-2380, p-ISSN: 2319-2372, Volume 6, Issue 5 (November to December 2013), pp.14-21

Article Link (Online Journal): www.iosrjournals.org

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

This study concludes that homogenous PRP gel showed excellent results in the healings of skin wounds in goats.However, this study could help veterinarians to consider the use of biomaterial substance in soft tissue healing.Further studies are necessary for the molecular investigation of healing process and proper establishment of commercially available of platelet rich plasma gel in our country and easiest way of application.

Knowledge Management: Journal