Dr. Md. Bahanur Rahman
Professor
Department of Microbiology and Hygiene, BAU, Mymensingh-2202
The present study was aimed to know the prevalence of peste des petits ruminants virus (PPRV) by studying the seroprevalence of PPRV specific antibodies in sheep, goats and cattle. For this study sera samples that were collected from domestic ruminant species from different rural areas and farms in Mymensingh, Bangladesh. None of the animals were less than 6 months of age and greater than 2 years of age and they had not been vaccinated with either TCRV or homologous PPR vaccine. The sera samples of 50 sheep were collected from different rural areas/farms and tested by C-ELISA. Of 50 sheep sera tested, 18 (36%) were seropositive and 32 (64%) were seronegative. The mean positive and negative antibody titers of sheep sera were 64.36%±3.60 and 19.99%±4.43 respectively. Of 120 goat sera tested, 59 (49.17%) were seropositive and 61 (58.83%) were seronegative. The mean positive and negative antibody titers in goat sera were 58.83%±2.79 and 18.61%±4.56 respectively. Of 300 cattle sera tested, 57 (19.05%) were seropositive and 243 (80.95%) were seronegative. The mean positive and negative antibody titers in cattle were 59.86%±4.72 and 21.10%±4.72 respectively. The highest and lowest mean seropositive antibody titers in sheep were 70.50% and 55.40% at chorniokhia and Roghurampur villages respectively, in goats 64.86%±5.74 and 55.45%±1.77 at Boyra east and churkhi respectively and that in cattle were 63.83%±4.46 and 58.23%±5.10 at chorghagra and churkhi respectively.
The source of seropositive antibodies can therefore only be from an unapparent infection or from immunity gained through recovery from a field out break in different seropositive areas in Mymensingh. In the absence of vaccination, the presence of PPRV antibodies indicated that PPR viruses were circulating in the population.
Prevalence, Isolation, Vaccine virus strain, PPRV
Some villages of Mymensingh district
Pest Management
1. Determination of seroprevalence of PPRV infection in sheep and goats.
2. Isolation, identification and characterization of PPRV prevalent in Bangladesh from filed cases.
3. Immunological characterization of the PPRV isolates and selection of vaccine strains.
4. Comparison of PPRV genome sequence with other PPRV isolates
a. Animals: Sheep and goats reared in semi-extensive and semi-intensive areas will be examined for the seroprevlence of PPRV.
b. Study areas: Samples will be collected mostly animals which brought to the Thana veterinary Hospital and semi-extensive farming areas.
c. Samples: Samples will be collected from suspected cases of infection and also from the contact animals.
d. Tissue culture: Vero cell will be used in this study for primary isolation and propagation of virus.
e. Competitive: ELISA kits: This will purchase from the world Reference Laboratory, Pirbright, U.K.
f. Reverse transcription polymerase chain reaction: for the detection PPRV RNA, RT-PCR will be performed.
Bangladesh Agricultural University Research Progress. Proceedings of the workshop 15-16 January 2005. Bangladesh Agricultural University Research System (BAURES), Mymensingh, Bangladesh.
225000
The research work was conducted on domestic ruminant species in different locations in Mymensingh district, Bangladesh to assess the prevalence of Peste des petits ruminant’s virus (PPRV) antibodies in apparently normal and non-vaccinated sheep, goats and cattle.
The PPR virus specific monoclonal antibody (Mab) based competitive ELISA (C-ELISA) technique was used to analyze 564 serum samples from 14 different surveyed areas for PPR antibodies.
Only three locations had animals that were free of antibody responses to PPRV disease. The seroprevalence of PPRV specific antibody varied between flocks, ranging from 10% to 100%. The level of PPRV specific antibody titre was higher in sheep (63.54) than in goats (58.71%) and cattle (59.86). the seroprevalences were 37.64% in sheep, 49.16% in goats and 19.05% in cattle. The overall percentage of PPR antibody responses to PPRV was 31.21%. The results of the study thus revealed that presence of seropositive PI value was due to natural infection by PPRV in the experimental animals of the surveyed locations. Lungs, lymphnodes and intestine from infected goats were collected and stored at 20C for PPRV isolation in Vero-cell culture. PPRV was detected in collected samples by ESA test.
Report/Proceedings