Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Apical Meristem Culture in Potato for Increasing Yield

M. J. Hossain
Tuber Crops Research Centre, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur.

M. K. R. Bhuiyan
Tuber Crops Research Centre, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur.

M. Zakaria
Tuber Crops Research Centre, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur.

A. K. M. A. Habib
Tuber Crops Research Centre,Bangladesh Agricultural Research Institute, Joydebpur, Gazipur.

Abstract/ Executive Summary:

Tissue culture method has been applied in five potato varieties to produce pathogen-tested stocks for improving productivity. Heat treatment of meristems affected culture viability negatively and virus freedom positively. In vitro response of both healthy and infected plants was almost similar. In vitro healthy plantlets after transplantation m the field showed excellent growth and yield performance compared to infected plantlets except survival percentage, number of stems per plantlet, days to establishment and tuberization, The plants raised with healthy tubers showed significantly better growth and tuber yield than the infected plants. Pathogen- tested plants produced about 42.7% higher yield than that of the infected plants.

Keywords: Apical meristem, Potato, Plantlets, Yield

Location: Bangladesh Agricultural Research Institute, Joydebpur.

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Potato

Objectives:

To produce disease-free potato stocks to revitalize yield potentials.

Methodology:

Infected tubers of five potato varieties such as, Cardinal, Diamant, Multa, Patrones and Kufri Sindhuri were used in this study. Virus infection was ensured by different testing methods. The tubers were planted in pots. Fifty percent plants were subjected to heat treatment at 36 ± 2^OC/30 ± 2⁰C for 16/8 h diurnal cycle for 35 days. Sample preparation, meristem excision, culture on Murashige and Skoog (MS 1962) media and incubation were done following the methods as already described. Forty meristems were included in each treatment. The developed rooted plantlets were again assayed by Latex and ELISA . In the first year, both healthy and infected in vitro plantlets were planted in two separate net house for comparison. In the second year, comparison was made with tuber plants. Plantlets were planted at 20 cm x 10 cm spacing, while that was 50cm x 25cm for tubers. Usual cultural practices were used to raise the crop with tubers, while the plantlets in the field were raised following the methods as reported. The crops were harvested at 90 days maturity. Data on various characters both in the laboratory and field were recorded and analyzed statistically. The percentage data were transformed into corresponding angles before analyzes.

Source of Information: Bangladesh Horticulture, 20 (1), Page: 29-33, June 1992 (ISSN 0379-4288)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

In vitro response of both healthy and infected plants was almost similar. In vitro healthy plantlets after transplantation in the field showed excellent growth and yield performance compared to infected plantlets except survival percentage, number of stems per plantlet, days to establishment and tuberization, The plants raised with healthy tubers showed significantly better growth and tuber yield than the infected plants. Pathogen- tested plants produced about 42.7% higher yield than that of the infected plants. From the above discussion, it is evident that a noticeable percentage of tuber yield is reduced due to virus infection which could be recovered by producing healthy tubers by tissue cultures.

Knowledge Management: Journal