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Survey of Isozyme Variation in Lablab Bean (lablab Purpureus L.)

Nasreen Sultana
Department of Horticulture Bangabandhu Sheikh Mujibur Rahman Agricultural University Gazipur-1706, Bangladesh

M. G. Rasul
Dept of Genetics and Plant Breeding, BSMRAU, Gazipur 1706, Bangladesh

Yukyo Ozaki
Laboratory of Horticultural Science, Faculty of Agriculture, Kyushu University, Fukuoka 812, Japan

Hiroshi Okubo
Laboratory of Horticultural Science, Faculty of Agriculture, Kyushu University, Fukuoka 812, Japan

Abstract/ Executive Summary:

A survey of 28 isozyme systems in 107 accessions of lablab bean representing 20 different countries with a wide range of morphological variation including determinate and indeterminate growth habit, wild and cultivated types including two most important and controversial regions of origin, India and Africa was done. Of 28 isozyme systems examined, clear and consistent banding patterns were obtained from 15 isozyme systems. Most of the isozymes dissolved well in three buffer types out of six used. Four polymorphic isozymes, GOT, 6-PGD, MDH and ME were detected. Isozyme variation was higher in African accessions than in Asian and in wild type than in cultivated types but no variation was found between determinate and indeterminate growth habits. This result might support that Africa is the probable place of origin of this crop.

Keywords: Lablab bean, Isozyme

Location: Gazipur

Start Date: 00-00-0000

End Date: 00-00-0000

Program Area: Variety and Species

Commodity: Lablab bean

Objectives:

To evaluate the genetic variation of lablab bean using isozymes as markers

Methodology:

One hundred and seven cultivars and genotypes representing 20 different countries collected from Bangabandhu Sheikh Mujibur Rahman Agricultural University, Bangladesh; Bangladesh Agricultural Research Institute (BARI), Bangladesh, Asian vegetables Research and Development Centre (AVRDC), Taiwan; Commonwealth Scientific and Industrial and Research Organization (CSIRO), Australia and Kyushu University Japan, were used

Source of Information: Bangladesh J. Pl. Breed. Genet., 18(2): 01-08, 2005

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Compatibility between isozyme systems and SGE buffer types are presented in Table 3. Of 28 isozyme systems examined, 15 isozyme systems, namely AMP, DIA, EST, ENP, GOT, IDH, LAP, MDH, ME, PER, PGI, PGM, 6-PGD, RBC and SOD produced clear banding pattern, eight isozyme system namely, ACP, ADH, ALT, GAL, GDH, GS, LDH and SKDH displayed hazy and nearly unreadable banding patterns and no staining activity was observed in the rest of the isozyme systems examined. Most of the isozyme dissolved well in buffer types B, C and D. Some isozymes displayed clear banding patterns with more than one buffer systems and banding patterns remained stable with each buffer type except for isozyme systems MDH and ME with buffer types B and C. Both MDH and ME displayed two regions of activities with buffer type B, whereas with buffer type C three regions were displayed in both isozymes. Among the three regions of isozyme ME, the third region was polymorphic .This result might support that Africa is the probable place of origin of this crop.

Knowledge Management: Journal