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Up-regulation of Stress Inducible Enzymes by Proline and Betaine in Maize Under Saline Stress

M.M. Rohman
Senior Scientific Officer
Plant Breeding Division BARI, Gazipur

A. Ahmed and
Scientific Officer
Plant Breeding Division BARI, Gazipur

M. R. Ali
Chief Scientific Officer
Plant Breeding Division BARI, Gazipur

Abstract/ Executive Summary:

The responses of catalase (CAT), important decomposer of H₂O₂, were examined under salinity stress to understanding the underlying stress tolerant mechanism. All the levels of salinity (4, 8, 12 and 16 dS/m) induced the CAT activities and the levels varied with stress duration. At 5 day and 10 day saline stress, 16 dS/m salinity caused maximum induction of CAT activity (127 and 83%, respectively) over control, where, 15 day stress showed 170% more activity over control. The foliar application of both proline and betaine provided better induction over salinity stress. However, in most of the cases, proline efficiently increased the CAT activities. On the other hand, the H₂O₂contents decreased by P and B suggesting that both P and B showed important roles in protecting maize cell from ROS by activating the expression of CAT.

Keywords: Up-Regulation, Stress, Enzymes, Proline, Betaine, Maize and Saline

Location: Gazipur

Start Date: 27-11-2012

End Date: 10-03-2013

Program Area: Variety and Species

Commodity: Maize

Objectives:

To know the abiotic stress tolerance mechanism under salinity stress.

Methodology:

Three days old seedlings of maize inbred CZ-10 were subjected to impose saline by adding NaCl at 4, 8, 12, 16 dS/m. Fifteen mM proline and betaine were applied as foliar spray daily twice. Protein was extracted from 5, 10 and 15 day stressed seedlings. To estimate soluble protein fresh plants were extracted by homogenizing in an equal volume of 25 mM Tris–HCl bu?er (pH 8.0) containing 1 mM EDTA, 1% (w/v) ascorbate and 10% (w/v) glycerol with mortar pestle. The homogenate was centrifuged at 11500 x g for 15 min, and the supernatant was used as a soluble protein solution. For H₂O₂assay fresh plants were extracted by homogenizing in 6X volume of 50 mM K-P buffer (pH 6.5). The homogenate was centrifuged at 11500g for 15 min, and 2.5 ml supernatant was used for further centrifugation at 11500g for 15 min with 833µl reaction mixture containing 5ml H₂SO₄and 15µl TiCl₄. The 2^nd supernatant was used for the assay of H₂O₂spectrophotometrically.

Source of Information: Annual Research Report 2012-13, Plant Breeding Division, BARI

Article Link (Online Journal):

Funding Source:

1. Government

Budget: 20,000

Total Budget (Tk.) : 20,000

Achievement/Findings:

All the salinity levels increased the H₂O₂ contents. The foliar application of proline and betaine decreased the H₂O₂ contents in all the treatments. However, in most of the cases, proline was more efficient in activating the CAT and decreasing the H₂O₂ contents. Therefore, the regulation of CAT activities are correlated with the H₂O₂ level in cell suggesting the decomposition role of CAT. However, besides CAT, glutathione peroxides and ascorbate peroxidase are also involved in ROS scavenging which thrust more research.

Knowledge Management: Report/Proceedings