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In Vitro Nmr Relaxation Study of Water Protons in the Intracellular Water of Eggplant

M. A. Rahman
Bangladesh Rice Research lnstitute, Gazipur, Bangladesh

A.K. M. S. lslam
Bangladesh Rice Rice Research lnstitute, Gazipur, Bangladesh

B. K. Bala
Agricultural University, Mymensingh, Bangladesh.

A. Khair
Jahangirnagar Univbrsity, Dlraka, Bangladesh

Abstract/ Executive Summary:

Xenon treated eggplant fruit was studied to examine the formation of structured water through nuclear magnetic resonance (NMR) measurements. Spin-lattice relaxation time (T₁) and spin-spin relaxation time (T₂), were carried out immediately after sample preparation and one day of xenon application, and continued for 15 days at the same temperature. It was found that the mean relaxation times T₁(921 ms) and T₂ (324 ms) were shorter in the xenon treated samples compared with the control ones, T₁ (995) ms) and T₂ (344 ms), respectively. Two phase behaviours were observed for both T₁and T₂, T₂ was also found to be independent to temperature. Browning of flesh was developed in the control sample after 6 days, while on sign of flesh browning was developed after 17 days in the treated sample. Formation of structured water by xenon gas results in suppression of the metabolic activity. Thus, xenon application was found to be effective n extending the storage life and maintaining the quality of fresh agricultural products.

Keywords: Xenon gas, Eggplant, NMR relaxation times (T1 T2), intracellular water, Browning

Location:

Start Date:

End Date:

Program Area: Postharvest and Agro-processing

Commodity: Brinjal

Objectives:

To investigate the suppression of metabolic activity try the formation of structured water in eggplant by measuring the proton NMR relaxation times, T₁ and T₂_.

Methodology:

The study was conducted during the month of September' 1997 in the laboratory room, in vitro study was performed at 20^oC in a tesla magnet corresponding to the proton resonance frequency of 25 MHZ. Eggplant fruits were collected from the farmers field. Fruits were stored within 1-2 hr of harvest in constant temperature chamber at 15^oC, before outset of the experiment. Samples without defects were selected. The samples were cut by the size of 5x5x30 mm3 immediately after cutting, the samples were, placed into the two NMR pressure tubes (10 mm in dia and 200 mm in length) and were tightly closed by the rubber cork. NMR relaxation measurements were performed with Jeol NMR spectrometer. This spectrometer was interfaced with a microcomputer for curve fitting. The whole magnetization decay curve was used for the calculation of the relaxation times. After initial measurement of T₁ and T₂, xenon gas was applied in one of the NMB tube tor 15 minutes at o.4o MPa at constant room temperature of 20^oC. ln other NMR tube, no gas pressure was applied and was treated as control. Samples were stored at 20^oC constant room temperature. T₁ values were determined by a repeated 90⁰-t 90⁰, saturation pulse sequence method where t is the time interval between two pulses and T, values were determined by 90⁰x-t-180⁰y-2t-180⁰y-2t Curr-Purcell-Meiboon-Gill (CPMG)pulse sequence method. The temperature was controlled with the accessory temperature control unit of the spectrometer and had an accuracy of about 4^oC + 1. All T₁ and T₂ values were the average of two independent measurements of 1 5 days. The relationship of the relaxation time (T,) with self-coefficient (D) and viscosity (n).

Source of Information: Pakistan Journal of Biological Sciences 5(1}: 88-90, 2002; Asian Network for Scientific lnformation 2002

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

ln conclusion the srx'n-lattice and spin-spin relaxation times, (T, and T2l were lower in xenon treated eggplant tissues than the control, The values of Tr are to be found similar to those documented for biological tissue of persimmon fruit. Two phaso behaviours were observed lor T, and Tr. The spin-spin ,elaxation time, T2 was f ound to be independenr to temperature, Tho decrease in T, and T2 showed that the water viscosity increased in xenon treated sample, which confirmed the structured water formation. Moreover, xenon treatrient also suppressed the browning for 15 days. Metabolic activity was suppressed in the xenon treated samples, Therefore, xenon gas could be used as an alternative storage method to increase the shelf lile and maintaining the quality of eggplants.

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