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Growth and Survival of Olive Barb, Puntius Sarana (hamilton 1822) Larvae Produced with Cryopreserved Versus Fresh Sperm

Shirin Akter
Department of Fisheries Biology and Genetics, Bangladesh Agricultural University, Mymensingh, Bangladesh

Mostafa Ali Reza Hossain
Department of Fisheries Biology and Genetics, Bangladesh Agricultural University, Mymensingh, Bangladesh

Md Nahiduzzaman
WorldFish, Bangladesh and South Asia, Rangpur, Bangladesh

Md Mahbubul Hassan
Department of Fisheries Biology and Genetics, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh

Abstract/ Executive Summary:

Despite the success in fertilization and hatching of fish eggs with cryopreserved sperm, report on growth and survival of larvae produced from frozen- thawed sperm is inadequate. The study evaluates the applicability of cryopreserved sperm for mass seed production by comparing the growth and survival of a popular food-fish olive barb, Puntius sarana larvae produced from cryopreserved and fresh sperm. The eggs were artificially fertilized with cryopreserved and freshly collected sperm, and the growth and survival of produced larvae from both group recorded up to 12 weeks. The independent sample t-test statistic showed the difference in lengths, t(718) = 0.241; P = 0.810 and weights, t (718) = 0.412; P = 0.680 were insignificant between two groups. There was also no significant difference, t(718) = 0.758, P = 0.448 in survival of larvae produced from cryopreserved and freshly collected sperm. The study indicates that larvae of olive barb produced from cryopreserved sperm are equally compatible in growth and survival as the larvae produced from fresh sperm. Therefore, cryopreserved sperm can be applied for artificial fertilization of P. sarana to supply quality seed for aquaculture.

Keywords: Cryopreserved sperm, Growth, Survival, Puntius sarana

Location: Faculty of Fisheries, Bangladesh Agricultural University.

Start Date: 00-07-2012

End Date: 00-11-2012

Program Area: Variety and Species

Commodity: Aquatic animal

Objectives:

1. To compare the growth and survival of P. sarana larvae produced with cryopreserved and fresh sperm.

Methodology:

Collection and rearing of larvae: The experiment was carried out during July to November, 2012 in backyard hatchery of the Faculty of Fisheries, Bangladesh Agricultural University. Two groups of larvae were produced by fertilizing olive barb eggs with freshly ejaculated sperm and frozen thawed sperm. After absorption of yolk sac, the larvae were transferred to three rectangular glass aquaria (120 9 50 9 35 cm) for each group. The initial density (number/L water) of the larvae was twenty five. Continuous aeration was supplied in the rearing tank, and water exchange took place once a week during the rearing period. The temperature throughout the study period was 24–31°C. The larvae were fed with finely minced hard-boiled chicken egg-yolk six times a day for first 3 days. During 3 days post-hatching period, larvae were fed to apparent satiation four times a day with live food (microalgae, zooplankton and crushed tubificid worm). After 4 weeks, live food was partially replaced with the pelleted starter feed (Paragon Feeds Limited, Bangladesh) until the end of the study period. The growth and survival of larvae was studied out up to 12 weeks rearing period. Ten larvae were randomly sampled once in a week with three replicates. The total length (mm) and wet weight (g) were taken for growth study. The lengths of the fish were measured using Vernier calipers (Mitutoyo 500-196-20, Foshan, China) to the nearest 0.05 mm, and body weights were measured to an accuracy of 1 mg using digital scale (UW1020H, Kyoto, Japan). The dead larvae were counted and removed from the tank every day, and the survival rate were calculated by counting all dead larvae over the rearing period. Calculation of growth and survival: Daily instantaneous length specific growth rates were calculated by fitting the exponential model: Lt = L_⁰e^gt, Where, Lt = total length (mm) at time t, L₀ = estimated length at hatching following the absorption of yolk sac, g = instantaneous growth coefficient, and t = estimated age (days after hatching). The weight specific instantaneous growth rates were calculated by fitting the exponential model: Wt = W₀e^gt, Where Wt = wet weight (mg) at time t, W₀ = estimated weight at hatching following the absorption of yolk sac, g = instantaneous growth coefficient, and t = estimated age (days after hatching). Length-weight relationship of larvae was calculated by fitting the exponential model: W = aL^b, where W is the body weight (mg) and L is the total length (mm). Parameters a is the intercept and b is the slope of equation based on natural logarithms: lnW = lna + blnL. Survival of larvae were calculated by fitting the exponential model Nt = N₀e^Zt , Where N₀ = estimated number of larvae at hatching, Nt = number of larvae at time t, Z = daily instantaneous mortality rate and t = estimated age (days after hatching). Data analysis: All the mentioned equations were log-transformed to calculate the slope and intercept by linear regression. The instantaneous growth coefficients of larvae produced with cryopreserved sperm were compared with fresh sperm using independent sample t-test. The survival was arcsine-transformed to induce homogeneity and analysed using independent sample t-test. The differences were considered statistically significant at a = 0.05. All data analyses were performed with SPSS version 20.0 (IBM Corporation, Armonk, NY, USA).

Source of Information: Aquaculture Research, 2014, 1–6

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Cryopreservation has deleterious effect on sperm quality, however, the development of larvae fertilized with thawed sperm was similar to that of fresh sperm. The present study suggests that cryopreservation technique can be applied to produce viable offspring of P. sarana without any difference in growth and survival of larvae. This would definitely contribute to the commercialization of cryopreserved fish sperm for hatchery operation and ex-situ conservation of the critically endangered olive barb.

Knowledge Management: Journal