Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Effect of Stocking Density on Growth and Survival of Hormone Treated Tilapia (oreochromis Niloticus) Fry Reared in Hapas-in-pond System

Nazneen Bagum
Bangladesh Fisheries Research Institute, Freshwater Station, Mymensingh-2201, Bangladesh

Muhammad Badrul Alam Shaheen
Department of Fisheries, The Peoples Republic of Bangladesh

Anuradha Bhadra
Bangladesh Fisheries Research Institute, Head Quarter, Mymensingh-2201, Bangladesh

Mohammad Ashaf-Ud-Doulah
Bangladesh Fisheries Research Institute, Freshwater Station, Mymensingh-2201, Bangladesh

Mohammad Showkat Hossain
Sylhet Agricultural University, Sylhet-3100, Bangladesh

Abstract/ Executive Summary:

An experiment was performed in hapas-in-pond to evaluate the effect of stocking density on the growth performances and survivability of monosex male Tilapia (Oreochromis niloticus) fry for a period of 28 days. Fry were collected, weighed and stocked inside a 1 m² hapa in a 0.24 ha pond with stocking densities of 1200 fry/hapa (T₁), 1700 fry/hapa (T₂) and 2200 fry/hapa(T₃). Each stocking were made in triplicate. They were fed with a 32% crude protein plus hormone (17 α-methyl testosterone) mixed feed 5 times a day. The physico-chemical parameters were recorded and found to be within suitable range for fish culture. The growth performances and survival of Tilapia (O. niloticus) fry were significantly (P<0.01) higher in T₁ than those obtained from T₂ and T₃, respectively. The FCR (feed conversion rate) were calculated lowest (1.56) in T₁ compared to T₂ and T₃, respectively. However, these results indicate that stocking density had a significant effect on growth performances and survival rates of monosex Tilapia (O. niloticus) fry.

Keywords: Growth performances, Oreochromis niloticus, Stocking density, Survival rate

Location: Shambhuganj, Mymensingh

Start Date: 20-06-2012

End Date: 18-07-2012

Program Area: Animal Health and Management

Commodity: Tilapia

Objectives:

1. To understand the effect of stocking density on the growth, FCR as well as survival in hapas-in-pond system of Monosex tilapia.

Methodology:

Study site: The experiment was conducted in a private fish farm named Ghinuk Fish Farm situated at Shambhuganj, Mymensingh, Bangladesh from 20 June to 18 July, 2012. Description of the experimental hapa: The experimental hapa (size of each hapa 1m²) were set at the corner of 0.24 ha pond and rest space of the pond was used by the hatchery owner for commercial purposes. All the hapas were rectangular of synthetic netting of mesh size 1.5 mm closed from all sides except the top. There was well organized inlet and outlet facilitates in the pond which hapas were set up. Design of the experiment: The experiment was conducted for a period of 28 days with three treatments each having three replicates. For convenience, hapas were arbitrarily numbered was 1 to 9. A total of 15300 O. niloticus fry (0.012±0.002 g) were collected and stocked in 9 hapas at three stocking densities such as 1200 fry/hapa (T₁), 1700 fry/hapa (T₂) and 2200 fry/hapa (T₃). Hormonal feed preparation and feeding: A hormone treated fish feed was prepared. The 17 α-methyl testosterone was the hormone used in the experiment. A stock solution was prepared by dissolving 0.06 g of hormone in 750 cm³ of 95% ethanol. Treatments were made by taking the accurate amount of the hormone from stock solution and brought up to 100 ml by addition 95% ethanol. This solution was evenly sprayed over 1 kg of 32% crude protein formulated feed (Quality feed) and mixed properly. The stocked fry in hapas were then fed with 17α-methyl testosterone hormone mixed formulated feed technique 5 times a day at an initial rate of 25% of their body weight and adjusted 10-12% of their body weight towards the end of experimental period. Growth measurement: Random sampling method was accomplished at an interval of 7 days to assess the growth and health status as well as for feed adjustment. At least 50 Tilapia fries were sampled from each hapa with the help of a scoop net. Physico-chemicals parameters: The physico-chemicals parameters such as water temperature, dissolved oxygen, pH, total alkalinity and ammonia-nitrogen (NH₄-N) was measured weekly from each hapa using a celsius thermometer, a portable dissolved oxygen meter (HI 9142, Hanna Instruments, Portugal) and a portable pH meter (HI 8424, Hanna Instruments, Portugal) and a portable ammonia test kitr (Hanna test kit, Portugal), respectively. Total alkalinity was determined following the titrimetric method according to the standard procedure and methods. Harvesting of experimental fry: After 28 days of rearing, O. niloticus fry were caught from each hapa with the help of a scoop net. The fry were counted for estimated survival rate and at least 50 fingerlings individually weighted to assess final growth in terms of weight, specific growth rate (SGR % day) and feed conservation ratio (FCR). Specific growth rate (SGR % day) and feed utilization efficiency were calculated. Data analysis: Comparison of treatment mean was carried out using one-way analysis of variance (ANOVA), followed by testing of pair-wise differences using Duncan’s Multiple Range Test. Significance was assigned at the 1% level. All statistical analysis was done by using the SPSS (Statistical Package for Social Science) version-13.5.

Source of Information: Trends in Fisheries Research Volume 4, Issue 1 (2015) ISSN: 2319–474X (p); 2319–4758 (e)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Stocking density had a significant effect on growth performances and survival of Tilapia (O. niloticus) fry. The growth and survivability were increased with the decreasing of density. Further studies are necessary for a longer period to determine the optimum stocking density for monosex Tilapia fry production at farm level.

Knowledge Management: Journal