Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

The Effects of Water Quality Additives on Bacterial Load and Production in Thai Climbing Perch (anabas Testudineus Bloch, 1792) Culture Pond

Mohammad Saifuddin Sumon
Fisheries and Marine Resource Technology Discipline, Khulna University, Khulna-9208, Bangladesh.

Md. Golam Sarower
Fisheries and Marine Resource Technology Discipline, Khulna University, Khulna-9208, Bangladesh.

Md. Rayhan Ali
Fisheries and Marine Resource Technology Discipline, Khulna University, Khulna-9208, Bangladesh.

Abstract/ Executive Summary:

This research was carried out to investigate the effects of commercial water quality additives named aqua cleaner and deotop on bacterial load in Thai climbing perch (Anabas testudineus) culture pond as well as to observe the effects of the additives on the production of climbing perch. The study was conducted under four treatments that were designated as T₁ (5 ml/decimal aqua cleaner and 61 g/decimal deotop,T₂ (10ml/decimal aqua cleaner and 122 g/decimal deotop),T₃ (20 ml/decimal aqua cleaner and 244 gm/decimal deotop),T₄(control without any additives) and each of them with three replicates.T₄ ([(4.99±0.03)×10⁴cfu/ml water], [(5.49±0.09)×10⁶cfu/g soil]) and T₃ ([(3.80±0.58) ×10⁴cfu/ml water], [(4.99±0.23) ×10⁶cfu/g soil]) revealed the highest and lowest average bacterial load in both water and soil of the culture pond respectively whereas the highest production of perch was found in T₂(10.23 kg/decimal) followed by T₁ (8.44 kg/decimal), T₃ (8.39 kg/decimal), and T₄ (7.71 kg/decimal).The result from this study suggests that the additives supplemented at a certain dose could decrease the excess bacterial load at a level results in production enhancement.

Keywords: Water quality additives, Thai climbing perch, Bacterial load, Production.

Location: Khulna University, Khulna, Bangladesh

Start Date:

End Date:

Program Area: Animal Health and Management

Commodity: Koi/Climbing

Objectives:

To investigate the effects of commercial water quality additives named aqua cleaner and
Deotop on bacterial load in Thai climbing perch (Anabas testudineus) culture pond as well as to observe the effects of the additives on the production of climbing perch.

Methodology:

Study Area and Experimental Design: This study was conducted by collecting data from research pond complex Fisheries and Marine Resource Technology Discipline at Khulna University, Khulna, Bangladesh. The experiment was covered 12 earthen ponds where all ponds were 1 decimal in size, 1.4 m in depth and all of them were rectangular in shape. All ponds were conducted under almost the same environmental factor like equal expose of sunlight as much as possible to avoid the extraneous effect on the experiment. All ponds were divided into 4 treatments and each treatment contained 3 replicates like - Treatment-one or T₁ (ponds one to three); Treatment-two or T₂ (ponds four to six); Treatment-three or T₃ (ponds seven to nine); Treatment-four orT₄ (ponds 10 to 12). The treatments consisted of three levels at an initial dose of aqua cleaner & deotop such as half dose (5 ml+61 gm)/decimal for T₁, recommended dose (10 ml+122 gm)/decimal for T₂, double dose (20 ml+244 gm)/decimal for T₃ and control without any dose (T₄), and were conducted every after 15 days. Pond Preparation, Fry Stocking and Feeding: The research ponds were dried before the stocking of fry. After pond water had been pumped out the ponds were exposed to sunlight for a period of about 20 days. Then liming was done for each pond at the rate of 180 g/decimal. After seven days of liming ponds were filled up with water up to 1.4 m. After that, the research ponds were well fenced by nylon net with the support of bamboo sticks. Then the fry of average 4g weight were stocked in the research ponds at a stocking density 500 fry in each pond (1 decimal). At the beginning of stocking, the feeding rate was 10% of their body weight that gradually decreased to 0% where feeding frequency was two times per day. Sampling and Data Collection: At an interval of 15 days about 50 fish were randomly caught with the help of fishing net from each pond and their weight was measured with balance. All the data recorded in a note book and spread sheet and finally calculated the average weight of fishes according to the treatment at every sampling day. In addition at each sampling period, the water and soil from each pond were also collected and total bacterial load in both of them were counted in Biochemistry and Molecular Genetics Lab, Khulna University, Khulna. Standard Plate Count (S.P.C) for Bacteria: In case of water sample, at first a definite amount of nutrient agar (11.75gm) was accurately weighted. Then it was taken in volumetric flask (500 ml) containing distilled water and autoclaved at a temperature of 121⁰C and pressure of 15 Ibs/sq inch for 20 minutes (ICMSF). Four dilution tubes with dilution strength were marked, 10^-1 to 10^-4. Then with a sterile pipette 1.0 ml from stock solution was mixed with 9 ml of sterile water in the dilution tube marked 10^-1 for tenfold (1 :10) dilution and by following serial dilutions further diluted up to 10^-4 times. A new, sterile pipette was used between each dilution and to mix the dilution tubes thoroughly each time. Then 1.0 ml of each dilution was transferred on sterile Petri dish (In duplicate). About 20 ml of autoclaved agar was poured and mixed thoroughly by rotating the Petri dish, first in one direction and then in the opposite direction. The writing on the plate bottoms was faced up and incubated them at 37⁰C for 48 hours. After incubation, the plates or Petri dish were analyzed. Analysis, in this case, means simply counting the entire colony forming units (cfu). The assumption that we have to make for this procedure is that each cfu originated from one individual microorganism. To get reliable results with the spread plate method, only those plates that have between 30 - 300 cfu were counted. As I counted colonies, marking them off with a Sharpie to avoid repeated counting. The number of cfu of bacteria in the sample is calculated. But in case of soil sample, just after the media preparation a stock solution of soil was prepared by adding 1.0 g of soil with 100 ml sterile water in a sterile conical flask. Then the similar procedure was followed as like as water sample. Data Handling Technique: Microsoft excel 2010 was used to determine mean, standard deviation as well as to build figure and SPSS 17 was used to determine coefficient of correlation. Coefficient of correlation was done at 5% significance level.

Source of Information: International Journal of Fisheries and Aquatic Studies 2015; 2(6): 235-238

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The result obtained from this experiment clearly reveal that the commercial water quality additives named aqua cleaner and deotop have a positive effect on the production of Thai climbing perch. In addition those additives play an important role to reduce the excess microbial load in both the soil and water results in the improvement of water quality of the culture pond that is prerequisite to get a better output in fish culture. It is possible to get about 3 kg more Thai claiming perch production per decimal by using those additives at a recommended dose in culture ponds. Thus those additives will be able to contribute in the fish production enhancement.

Knowledge Management: Journal