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Nutritional Susceptibility to Morphological, Chemical and Microbial Variability: an Investigation on Mud Crab, Scylla Serrata in Bangladesh

Bijoya Paul
Department of Fisheries, University of Dhaka, Dhaka-1000, Bangladesh.

Dewan Ali Ahsan
Department of Fisheries, University of Dhaka, Dhaka-1000, Bangladesh.

Roland Nathan Mandal
Department of Zoology, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh.

Md. Hasan Faruque
Department of Fisheries, University of Dhaka, Dhaka-1000, Bangladesh.

Abstract/ Executive Summary:

Nutritional susceptibility of Scylla serrata to chemical and microbial variability with varying morphological capacity was the thematic area of the present investigation. In detail, morphological capacity considering carapace length, carapace width, total length, chelate leg length and crawl leg length; the nutritional composition considering protein, lipid, ash, moisture; chemical assessment considering TVN, TMA; and microbial assessment considering TBC, TMC were measured for both male and female mud crab. Morphological observation revealed that the weight of male mud crab was changed by 65% with changing per unit of length (b=0.65) and in female it was by 42%. Moreover, for males the b values of weight-condition factor and length-condition factor ranges from above 2 to above 3; whereas in females the range of values were less than that found in males. TBC and TMC found in muscle of male mud crab were higher than that of female. The beta co-efficient of multivariate regression analysis showed (TVN= 9.2+0.48 TMA+0.02 TBC+ 1.7 TMC) that the TVN is changed by 0.48 unit/TMA, 0.02 unit/TBC and 1.7 unit/TMC changes indicating that the TVN value is highly changed with changing TMC followed by TMA and TBC. In case of TMA, the beta co-efficient of multivariate regression analysis (TMA=52.859+ 0.032TBC+3.624TMC) showed that the TMA is changed by 0.032 unit/TBC and 3.624 unit/TMC changes indicating that the TMA value is highly changed with changing TMC rather than that of TBC. The protein content found to negatively relate with the moisture content by 0.661 coefficient values and with lipid with 0.04 values. The correlation co-efficient of proximity composition considering primarily protein and lipid with chemical and microbial variability showed that protein is negatively sensitive to TVN, TBC and TMC values. It can then be inferred that the nutritious proximity of mud crab is significantly susceptible to sex with varying morphological capacity, chemical and microbial variability.

Keywords: Mud crab, Nutritional susceptibility, Morphological variability, Proximate composition

Location: Dhaka, Bangladesh

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Crab

Objectives:

1. To know the nutritional susceptibility of Scylla serrata to chemical and microbial variability with varying morphological capacity.

Methodology:

The experimental specimen (both male and female of Scylla serrata) were collected at rate 100 Taka/kg from Farmgate Bazar Fish Market, Dhaka. The collected samples were washed in chlorinated water and preserved in a deep freezer (- 20 ºC); afterwards those were taken out for performing analysis. Each type of sample was divided into four lots. One used for proximate composition, one for chemical assessment, one for microbial quality and another lot was for determination of morphological characteristics. Morphological analysis: The collected mud crabs were analysed for recording the morphological capacity considering carapace length, carapace width, total length, chelate leg length and crawl leg length. Individual carapace width (CW) between tips of the longest lateral spines across the middle line between the frontal notch and posterior margin were measured using a Vernier caliper (0.1mm accuracy) and individual weight was taken in Docbel (BRAUN) weighing balance (Accuracy of 2g) after removing all adhering water from the body using a blotting paper. Biochemical composition: Moisture, lipid, protein and ash contents were determined. Protein was estimated by kjeldahl’s method.Fat was determined by Soxhlet’s method. The percentage of ash content was estimated by burning the materials in a Muffle Furnace at 55-60°C for 4-6 h and moisture was determined by drying the samples at 100-105°C in an oven. Chemical assessment: Total Volatile Nitrogen (TVN) and Trimethylamine (TMA) were investigated using AOAC official method to observe chemical changes. Conway Micro Diffusion technique was employed for the estimation of TVN and also TMA to observe chemical changes. Microbial assessment: The microbiological changes in samples such as total bacterial count (TBC) and total mould count (TMC) were determined by decimal dilution technique followed by pour plating. Analysis: The length-weight relationships were determined separately for males and females in Scylla serrata by the linear equation Y = a + bX where X= independent variable, Y = dependent variable and ‘a’ and ‘b’ are constants. For this purpose, the observed values of individual crabs were transferred into logarithmic values and regression analysis was carried out to calculate the ‘a’ and ‘b’ values. The variation in male and female S. serrata was calculated using ANOVA (Analysis of covariance). Descriptive statistics was used to analyze and represent the data. Statistical analysis was executed with the SPSS software package (verson11.5, SAS Institute Inc, USA) and with Microsoft Excel.

Source of Information: International Journal of Fisheries and Aquatic Studies 2015; 2(6): 313-319

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Mud crab shows a sexual dimorphism in its microbial, chemical and nutritional proximity and male mud crab has tendency to be heavier when compare to the female. Spoilage of both male and female mud crab is increased with increasing TMA, TBC and even with TMC values. It has also found that the nutritional value changes with changing spoilage indicator, and even chemical and microbial quality. It is, thus, concluded that the nutritious proximity of mud crab is significantly susceptible to sex with varying morphological capacity, chemical and microbial variability.

Knowledge Management: Journal