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Endoparasitic Infestation in Channa Punctatus (bloch, 1793) Collected from Different Water Bodies in Sylhet

S. M. I. Khalil
Department of Fish Health Management, Sylhet Agricultural University, Sylhet-3100, Bangladesh.

M. N. Haque
Department of Fish Biology and Genetics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

S. M. Bari
Department of Fish Health Management, Sylhet Agricultural University, Sylhet-3100, Bangladesh.

M. A. A. Mamun
Department of Fish Health Management, Sylhet Agricultural University, Sylhet-3100, Bangladesh.

Abstract/ Executive Summary:

The present study was conducted to determine the endoparasitic infestation and their effects on the growth of Channa punctatus from different water bodies in Sylhet during the period from January 2013 to May 2013. Six different species of parasites identified from the hosts examined were i). Euclinostomum multicaecum, ii). Allocreadium handiai, iii). Genarchopsis dasus, iv). Isoparorchys hypselobagri, v). Camallanus intestinalus and vi). Pellisentis ophiocephali. Moderate infestations were found in C. punctatus due to parasites. Prevalence was different in different months. The highest prevalence observed in January (75.00%) and lowest in February (55.56%). Changes in the nature of growth and loss of weight as a result of parasitic infestation were noticed. Accordingly length, weight and condition factors were found greatly affected. Loss of total length was 2.68%. The loss of weight and condition factor was 10.45% and 0.03, respectively. The loss of gonad weight also observed in infested male and female host and it was 5.25% and 3.64%, respectively.

Keywords: Endoparasitic, Infestation, Channa punctatus and Haor

Location: Disease Laboratory, Department of Fish Health Management, Sylhet Agricultural University, Sylhet.

Start Date: 00-01-2013

End Date: 00-05-2013

Program Area: Animal Health and Management

Commodity: Aquatic animal

Objectives:

To see the prevalence and intensity of endoparasites as well as variation of infestation in male and female fishes (C. punctatus) from different water bodies in Sylhet.

Methodology:

Sampling: The host fish species of Channa punctatus were collected from different haors and markets from Sylhet during the period from January 2013 to May 2013. Live and fresh fishes were brought to the Disease Laboratory, Department of Fish Health Management, Sylhet Agricultural University, Sylhet for investigation. Parasitological studies: The measurements of total length (TL), standard length (SL), head length (HL), body weight (BW) and gonad weight (GW) of fish were done by centimeter scale and the electric balance respectively. Then the fishes were dissected in order to collect the endoparasites. An incision was made along the mid-ventral line of the fish. The surfaces of the visceral organs, body cavities and serous membranes were examined for encysted larvae and parasites using hand lens. All of the organs were removed intact and carefully from the body and put into formalin solution in petridishes. After separating, the internal organs (stomach, intestine, liver and body cavity) were examined individually for parasite in separate petridishes with formalin solution. The stomach and intestine were carefully opened by an incision and then were shaken to dislodge the parasites that might remain attached to the lining of the epithelium by their head ends. The epithelial layers of the stomach and intestine were scrapped with a scalpel to remove any parasite that might remain attached to the layers, and the liver and body cavity were shredded with a pair of forceps and needles. The collected parasites were then washed by fresh saline solution. The contents of each petridish were then stirred well and allowed to settle in the bottom of the petridish. The sediment was then examined with a dissecting microscope. The collected parasites were washed by fresh water to clean any debris before making temporary mounts or permanent slides. For the purpose of fixation of nematode and acanthocephalan parasites hot glacial acetic acid and AFA (Alcohol Formalin Acetic) were used, respectively. The collected parasites were placed in hot fixative and left there for a few minutes. After fixation, the parasites were preserved in 70% ethyl alcohol in vials for prolonged storage. Lactophenol was used in order to clean the nematodes and acanthocephalan parasites. The nematodes were kept in lactophenol for five to seven days for visibility of the internal organs. The acanthocephalans required four to five days to be cleaned of in lactophenol. The clean parasites (nematodes and acanthocephalans) were mounted on slides temporarily in lactophenol. To make permanent slides of acanthocephalan the parasites were stained with borax carmine for one and half to two hours and then after dehydrating in alcohol graded series of 35%, 50%, 70%, 85%, 95% and 100%, the parasites were cleaned with xylene and mounted in Canada balsam.

Source of Information: J. Syl. Agril. Univ. 1(1):59-64, 2014 ISSN: 2308-1597

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The investigation was conducted for five months and a marked difference was found between infested and uninfested fish. The nematode and digenetic trematode was dominant among other groups of parasite. Prevalence and intensity were moderate in host fish. Total length, body weight, gonad weight and condition factor were lost due to parasitic action in infested fish. However the study should continue for one year for better understanding of the parasitism and effects of parasite on host.

Knowledge Management: Journal