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Comparative Study of Carp Pituitary Gland (pg) Extract and Synthetic Hormone Ovaprim Used in the Induced Breeding of Stinging Catfish, Heteropneustes Fossilis (siluriformes: Heteropneustidae)

Md. Belal Hossain
Department of Fisheries and Marine Science, Noakhali Science and Technology University, Sonapur 3802, Bangladesh

Md. Mosaddequr Rahman
Department of Fisheries, Faculty of Agriculture, University of Rajshahi, Rajshahi 6205, Bangladesh

Md. Golam Sarwer
Department of Aquaculture, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh.

Md. Yusuf Ali
Department of Aquaculture, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh.

Ferdous Ahamed
Faculty of Fisheries, Kagoshima University, 4-50-20 Shimoarata, Kagoshima 890-0056, Japan

Sharmeen Rahman
Department of Fisheries, Faculty of Agriculture, University of Rajshahi, Rajshahi 6205, Bangladesh

Bernerd Fulanda
Kenya Marine and Fisheries Research Institute, Marine and Coastal, P.O. Box 81651-80100, Mombasa, Kenya

Mohammad Mustafizur Rahman
Kulliyyah of Science, International Islamic University Malaysia, Jalan Istana, Bandar Indera Mahkota, 25200 Kuantan, Pahang, Malaysia.

Bharat Raj Subba
Department of Zoology, Post Graduate Campus (TU), Biratnagar, Nepal

Md. Yeamin Hossain
Department of Fisheries, Faculty of Agriculture, University of Rajshahi, Rajshahi 6205, Bangladesh

Abstract/ Executive Summary:

The present study compared the effectiveness of the Carp pituitary gland extract (PGE) and the synthetic hormone Ovaprim on spawning success of the stinging catfish, Heteropneustes fossilis during induced breeding. The PGE hormone was administered at 6 mg/kg of body weight for females and 2 mg/kg of body weight for males. In contrast, Ovaprim was administered at 0.3 ml/kg body weight and 0.1 ml/kg of body weight for females and males, respectively. The spawning success was higher in the Ovaprim-induced individuals with better performance recorded at all stages of spawning including latency period, ovulation, fertilization, hatching and incubation period compared to the PGE-induced individuals. In the Ovaprim induced individuals, the latency period occurred within 10 hours while in PG induced individuals, the latency was after 15 hours. Similarly, ovulation rate was 90% for Ovaprim injected fish but lower 78.7% for PGE injected fish. Higher rate of fertilization was observed in the eggs of Ovaprim treated fishes 86.7% compared to 69.2% in PGE induced fish. On the other hand, hatching rate was 76.9% in eggs spawned from Ovaprim induced individuals compared to 72.7% in PGE induced fish and the incubation period was also shorter at 3.5 h for eggs from Ovaprim-induced fish while the PGE induced fish eggs required a 5-h incubation period. Finally, the results showed that Ovaprim treated fish yielded better results compared the PGE treated fish in terms of ovulation, fertilization and hatching rates of H. fossilis.

Keywords: Induced spawning; Heteropneustes fossilis; Pituitary gland extract; Ovaprim; Ovulation rate;

Location: Feni district, Bangladesh.

Start Date: 00-02-2010

End Date: 00-07-2010

Program Area: Variety and Species

Commodity: Cat fish

Objectives:

To compare the effectiveness of the Carp pituitary gland extract (PGE) and the synthetic hormone Ovaprim on spawning success of the stinging catfish, Heteropneustes ?fossilis during induced breeding.

Methodology:

Study site and experimental design: The present study was conducted at the Rakamary fish hatchery which is one of the leading hatcheries in Feni region of Bangladesh during February 2010 to July 2010. Brood Collection: The brood fish for the artificial breeding of H. fossilis were obtained from the Rakamary fish hatchery. In this study, the male fish used ranged from 15 to 20 cm in total length and 30 to 70 g in weight. On the other hand, the female fish used ranged from 17 to 25 cm in total length and 40 to 150 g in weight. All the broodstock was checked for diseases and acclimatized before the induced breeding procedures and were kept separately in ponds of 14.3 × 8.15 × 1.5 m for four months before the start of the breeding season. Brood stock management: The brood fish were fed on a supplementary diet formulated from 25% fish meal, 20% rice bran, 20% wheat flour, 15% mustard oil cake, 4% molasses and 1% vitamin premix. The brooders were reared for four months with feeding at two times a day at the rate of 5-6% of the body weight. Additionally, the ponds were treated with animal manure at 15 days interval at the rate of 1250 kg/hectare. Similarly, artificial fertilizer application was done using Urea and Triple Superphosphate (TSP) at the rate of 50 kg/hectare and 25 kg/hectare, respectively. Brood selection and conditioning: Brooders were collected from the rearing ponds using a cast net in the morning between 8:00-9:00 am on the day of the breeding trials and immediately transferred to a circular tank in the hatchery. The males and females were kept in separate tanks and continuous water flow was maintained to ensure sufficient aeration. However, no feeding was conducted during the conditioning period. Brood stock injection and breeding induced: Commercially available dehydrated Carp pituitary gland (PG) extracts and Ovaprim were used as inducing agents. The body weight (g) of each brooder was weighed on an electronic balance (College B204-S, Switzerland) to estimate the required amount of PGE for the induction. The brooders were divided into groups of three females and five males of H. fossilis each, and then subjected to hormone treatment: one group was injected using PGE and the second group using Ovaprim hormone. The PGE hormone was administered at 6 mg/kg of body weight for females and 2 mg/kg of body weight for males. On the other hand, Ovaprim was administered at 0.3 ml/kg body weight and 0.1 ml/kg of body weight for females and males, respectively. For all treatments, the hormone was administered by intra-muscular injection on muscles beneath the dorsal fin slightly above the lateral line. After injection, the brooders were kept in separate breeding tanks for each treatment. Breeding and eggs transfer for incubation: After injection, all the brooders were found to be ovulated after a period of 8-15 h. The brooders were then transferred from the holding tanks after the completion of ovulation. The fertilized eggs transferred into mini rectangular hatching trays while taking precaution to avoid damage and fungal/bacterial contamination during the egg collection process. The number of eggs released into each tray was estimated using gravimetric methods. Thereafter, a continuous flow of water was maintained for aeration to ensure the environmental conditions were optimal for the hatching process. Determination of ovulation, fertilization and hatching rate: Ovulation rate, fertilization rate and hatching rates were calculated using the following formula: Ovulation rate (%) = No. of fish ovulated/Total no. of fish injected× 100 Fertilization rate (%) = No. of fertilizedeggs/ Total no. of eggs x100 Hatching rate (%) = No. of eggs hatched/Total no. of fertilized eggs x100 Statistical analyses: Data and statistical analyses were performed using GraphPad Prism 5 software. A Chi square test was used to check the ovulation, fertilization and hatching rates between Ovaprim and PGE treated fishes. All statistical analyses were considered significant at 5% (p<0.05).

Source of Information: Our Nature (2012) 10: 89-95

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The Ovaprim treated (0.3 ml/kg body weight for females and 0.1 ml/kg of body weight for males) fish yielded better results compared the PGE treated (6 mg/kg of body weight for females and 2 mg/kg of body weight for males) fish in terms of ovulation, fertilization and hatching rates of H. fossilis during this study. These results would be useful for apposite management of induced breeding in H. fossilis or any catfish.

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