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Maturation and Spawning of Penaeid Shrimp Penaeus Monodon Fabricius Collected from Off Shore Water of the Bay of Bengal

SHEIKH AFTAB UDDIN
Institute of Marine Sciences,University of Chittagong, Bangladesh

NANI GOPAL DAS
Institute of Marine Sciences,University of Chittagong, Bangladesh

ABU HENA MUSTAFA KAMAL
Institute of Marine Sciences,University of Chittagong, Bangladesh

Abstract/ Executive Summary:

In total 65 gravid females of Penaeus monodon were collected from off shore waters of Bangladesh for the study of maturation, spawning and hatching performances. To promote ovarian maturation and spawning of P. monodon eye stalk ablation and control of physical factors of the environment were taken into consideration. The number of eggs depends on the weight of the gravid female that varied from 70,000 to 375,000 having the body weight of 65 to 120 g where the average number of eggs was 170,300. 77.26% of the eggs were fertilized and 83.28% of them produced nauplii.

Keywords: Maturation, Spawning, Hatching, Penaeid

Location: Prime Shrimp Hatchery Limited, Cox’s Bazar.

Start Date: 00-01-2001

End Date: 00-06-2002

Program Area: Variety and Species

Commodity: Shrimp

Objectives:

To develop a reference database on the ovarian development by the eye–stalk ablation and hatching technique of P. monodon collected from the offshore water of Bangladesh.

Methodology:

This study was carried out from January 2001 to June 2002 in Prime Shrimp Hatchery Limited, Cox’s Bazar, where adequate research facilities for the study of hatchery operation and management were readily available. The broodstocks were collected from the south of south patches, near St. Martin’s Island of the Bay of Bengal using deep sea fishing trawler. The duration of each haul was about 2 h and the sorted matured male and female were packed at 4–5 inds./bag with proper aeration before carried to the hatchery. For maturation, spawning and hatching the water quality was maintained at 28–33 ppt salinity, 28–32⁰ C temperature and 7.8–8.5 pH. The supplied seawater was filtered by using slow sand filter, cartridge filter and UV to remove the bacteria and other undesired particles. The water level in the maturation tanks was kept at 70 cm with a constant flow at about 10 L/minute that provides 200% exchange/day. The continuous aeration was provided in the maturation, spawning and hatching tanks treated with 4 ppm oxytetracycline. About 10% of the biomass was fed to the shrimp and the constituents of feed were crabs, squid, clam, beef liver etc. Before ablation, both males and females were kept in two separate broodstock tanks containing 500 L of sea water. The males were transferred to the maturation tank after treating with antibiotic (20 ppm Oxytetracycline) for half an hour. Each female sorted from the holding tank of females was removed and hold it one hand with head facing away from the body and checked both the eyes for any damage. At first, the damaged eye was ablated. Ablation was carried out unilaterally with a heated surgical forceps. This seems to be the best procedure to keep the hemolymp from percolating from the wound. The body weights of the selected females varied from 65–120 g. After ablation females were placed in indoor tanks containing antibiotic solution (20ppm) for half an hour and then released to the maturation tank. The number of shrimp per maturation tank (20 ton capacity) was around 25 with a sex ratio of male and female 1:2. The ovarian development was examined by shining light against the side of the abdomen with a waterproof hand light, particularly in the area behind the cephalothorax. The gravid spawner was dipped for about 5 minutes in 500 ppm formalin. After formalin bath the female was rinsed with sea water and placed into the spawning tank. In each spawning tank (1 ton) a gravid female was placed and treated with 10 ppm EDTA and 4 ppm Ciprofloxin. After spawning the spent females were removed from the tanks by a scoop net. The tank water was drained and the eggs were passed through a 350 μ hand net which retains feces and they were collected on a 159 μ hand net in a bucket. Before transferring the eggs to the hatching tanks, they were washed thoroughly with running seawater at least for 10 minutes and then they were again treated with 10 ppm EDTA and 4 ppm Oxytetracycline. The number of eggs and the percentage of fertilized eggs were estimated. The hatching rate was determined by using the following formula: H%=Y/X x100%; H=hatching rate, Y=total number of nauplii and X=total number of eggs.

Source of Information: INTERNATIONAL JOURNAL OF AGRICULTURE & BIOLOGY Vol. 7, No. 6,925-927, 2005

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The number of eggs varied from 70,000 to 375,000 having the body weight of 65 to 120 g where the average number of eggs was 170,300. 77.26% of the eggs were fertilized and 83.28% of them produced nauplii.

Knowledge Management: Journal