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Investigation Into Gyneco-pathological Disorders and Identification of Associated Bacteria from the Genital Organs of Cows in Dinajpur, Bangladesh

Dr. Md. Amirul Hasan
Scientific Officer
Triaing, planing & Technology Testing Division, Bangladesh Livestock Research Institute, Savar, Dhaka

Abstract/ Executive Summary:

The present study was aimed to investigate the gynaeco-pathological disorders by post-mortem and histopathological examination, and to identify the associated bacteria. A total of 310 genital tracts of cows were collected from slaughter house of Dinajpur Sadar Upazilla during April 2009 to March 2010. Among the 310 samples, 31.29% (n=97/310) were affected with endrometritis. Similarly, 8.37% (n=26/310) cystic ovary, 6.77% (n=21/310) ovary hyperplasia, 4.84% (n=15/310) pyometra, 4.84% (n=15/310) parovarian cyst, 4.52% (n=14/310) hydrometra, 4.84% (n=15/310) ovary hypoplasia, 3.55%(n=11/310) ovaro-bursal adhesion, 1.29% (n=4/310) vaginal cyst and 0.66% (n=2/310)) hemorrhagic uterine horn were detected by postmortem examination, the cases were reconfirmed by histopathological studies. Uterine fluid (n=50) samples were collected, and were subjected for conventional bacteriological culture and biochemical analysis. Escherichia coli and Salmonella sp. could be identified from 30% (n=15/50) and 8% (n=4/50) samples, respectively. In conclusion, various pathological disorders in the female reproductive system of cows are prevalent, that may cause reduction of calf production.

Keywords: Bacteria, Cows, Genital organs, Histopathology, Identification, Post-mortem

Location: Laboratory at the Department of Microbiology, and the Department of Pathology and Parasitology of Hajee Mohammad Danesh Science and Technology (HSTU), Dinajpur

Start Date: 00-04-2009

End Date: 00-03-2010

Program Area: Animal Health and Management

Commodity: Pollen grain

Objectives:

To investigate the gynecopathological disorders of cows and identification of the associated bacteria with genital organs of cows

Methodology:

Experimental area and animals: Mostly slaughtered animals in slaughter house in Dinajpur Sadar Upazilla were used for collection of samples. The animals aged between 6-8 years. Most of the slaughtered animals were of ill health. Their weight varied from 70-100 kg. Sample collection: A total of 310 cattle were examined and female genital tract samples such as uterus, vagina, cervix and ovary were collected from the different abattoirs houses of Dinajpur Sadar Upazilla, Bangladesh, and the samples were transferred to the Laboratory at the Department of Microbiology, and the Department of Pathology and Parasitology of Hajee Mohammad Danesh Science and Technology (HSTU) for necropsy, histo-pathological examination, and isolation and identification of the associated bacteria. Post-mortem examination: The gross examinations of the female reproductive tract followed by post-mortem examinations of all the samples were performed. All the reproductive tracts were examined carefully. The ovaries were examined for their functional activity. Histo-pathological examination: During necropsy, various organs having gross lesions were collected, and were fixed in 10% buffered neutral formalin for histopathological studies. The formalin fixed tissue samples were then processed and stained as per standard method. Isolation and identification of bacteria: Culture media used for isolation and purification of bacteria included: EMB agar, MacConkey agar, SS agar and nutrient agar (Oxoid, England). Inoculated media were incubated aerobically at 37ºC for 24 h. The bacteria were then identified by studying their morphology, Gram staining characters, and biochemical tests. Biochemical test: The bacterial isolates were identified by standard biochemical tests such as MIU test (Motility, indol and Urease test), TSI (triple sugar iron), Voges Proskauer (VP) test.

Source of Information: BLRI. Annual research review Workshop, 2009-10. BLRI, Savar, Dhaka.

Article Link (Online Journal):

Funding Source:

1. Government

Budget: What is the budget of the project?

Total Budget (Tk.) :

Achievement/Findings:

Various pathological disorders in the female reproductive system may affect the reproduction performance. Further, it may be pointed out that various pathological disorders in female reproductive system are increasing menacingly along with introduction of cross breeding program through artificial insemination. The problem may be aggravated due to inadequate veterinary coverage and gynecological knowledge, which needs more attention.

Knowledge Management: Report/Proceedings