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Physico-chemical Analysis of Mango (mangifera Indica L.) Germplasm Available in the South-western Region of Bangladesh

M.A. Hossain
Agrotechnology Discipline, Khulna University, Khulna, Bangladesh

M.A. Mannan
Agrotechnology Discipline, Khulna University, Khulna, Bangladesh

S.K. Roy
Assistant professor
Agri. Studies, Khulna Public College, Boyra, Khulna

P. Shil
Lecturer
Agri. Studies, Khulna Model College, Boyra, Khulna

Abstract/ Executive Summary:

Mango (Mangifera indica L.) being highly cross pollinated crop possesses inestimable types with different fruit characteristics. The present study has attempted to account for the natural variability existing in physico-chemical properties mango germplasm (Gp) of the south-western region of Bangladesh. A significant variation in physiochemical characters was observed among the 90 germplasm. In case of the quantitative traits, higher variability was observed for the characters like fruit weight (14.46%), skin weight (10.06%), and stone/seed depth (8.30%) while other parameter showed moderate variation. Of the qualitative characters studied, a high coefficient of variation was measured for anthocyanine content (30.03%) and T-acidity content (8.04%). Moderate coefficient of variation was observed for Carotinoids (7.16%), TSS/TA (7.63%) and Ascorbic acid (6.96%) content while other parameter showed lower variation. Highest anthocyanine content (0.41 mg/100g) and lowest (0.09mg/100g) was noticed in Gp 33 and Gp 44, respectively while highest carotenoids content was recorded in Gp 44 (17.36 mg/100g) and lowest in Gp 51 (1.30 mg/100g). The present study showed high to moderate physiochemical especially high ascorbic acid, flavonoids, carbohydrate and carotenoids content suggested that the Mango can be used as a good source preparation of health drink. The germplasm may be important commercial mango fruit, grown and available almost everywhere in Bangladesh, can help overcoming the malnutrition problem in our country.

Keywords: Physico-chemical analysis, Germplasm, Mangifera indica, South-western region of Bangladesh

Location: The laboratory of Molecular Horticulture, Agrotechnology Discipline, Khulna University, Khulna-9208

Start Date: 00-08-2010

End Date: 00-12-2011

Program Area: Quality and Nutrition

Commodity: Mango

Objectives:

1. To identify, collection and evaluation of the selected mangoes germplasm which are most commonly cultivated in South-western region Bangladesh,

2. To compare the qualitative and quantitative characteristics of mango germplasm, and

3. To determine the phytochemical characteristics of the selected mangoes germplasm

Methodology:

The study “Evaluation of Physico-chemical and Molecular Variability of Mango Germplasm available in South-western region of Bangladesh” was carried out during the periods of August 2010 to December 2011 in the laboratory of Molecular Horticulture, Agrotechnology Discipline, Khulna University, Khulna-9208, Bangladesh. In the present study, 90 Mangoes germplasm selected from south-western part of Bangladesh where as the south-western region of Bangladesh covered for the study included Germplasm Centre Khulna University, Khulna, Satkhira and Jessore district falling in coastal zone, and enjoying an agro-climatic condition of the state. The trees were marked to facilitate frequent visits if fruits of desired stage were not available during the initial trip. The fruits were harvested at mature unripe stage and then the collected fruits were brought to the laboratory of Molecular Horticulture and kept in ambient condition for ripening to follow the physicochemical characters. Physical properties were recorded as i.e.; the weight of fruit; seed and skin were taken in gm by electric balance. Fruit length, fruit breadth, fruit width, seed length, seed breadth and seed width were estimated in centimeter by using measuring scale and slide calipers. The methods for the estimation of pH, TSS, titratable acidity, ascorbic acid (Vit-C), carotenoids, anthocyanin, carbohydrate and flavonoids of fruit pulp and iron determine mature dry sample were followed. The pH of fruit pulp was measured by a desktop pH meter (pHep Hanna, Italy). The methods for the estimation of the parameters were followed. Total soluble solids (TSS) content was determined by hand refractometer (ATAGO, Japan). The methods for the estimation of the parameters were followed. Temperature correction was made. Ascorbic acid quantified and followed standard procedure given by A.O.A.C.. Carotenoids content was determined. Anthocyanin is extracted with ethanolic-hydrochloride and the intensity of the colour appeared is measured calorimetrically. Flavonoid was estimated. Anthocyanin is extracted with ethanolichydrochloride and the intensity of the colour appeared is measured calorimetrically. From the graph we calculated the amount of carbohydrate present the sample tube. Ascorbic acid was quantified. Iron (Fe) content determined mature dry sample were followed. In estimating iron, it is at first brought ferric form by the use of oxidizing agent; i.e, potassium persulphate. After conversion, it is reacted with potassium thiocyanate to form red coloured ferric thiocyanate complex. The intensity of the color appeared is then measured Colorimetrically. The experiment was laid out in completely randomized design (CRD) with three replications. The collected data from physical, biochemical and organolaptic properties were statistically analyzed by analysis of variance (ANOVA). Duncan’s multiple range test (DMRT) was used to compare the means of different parameters and the means were calculated by using “MSTAT-C”.

Source of Information: Bangladesh Res. Pub. J. 11(3): 242-251, June - August 2015; ISSN: 1998-2003

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The present study has attempted to account for the natural variability existing in physicochemical properties mango germplasm (Gp) of the south-western region of Bangladesh. A significant variation in physiochemical characters was observed among the 90 germplasm. In case of the quantitative traits, higher variability was observed for the characters like fruit weight (14.46%), skin weight (10.06%), and stone/seed depth (8.30%) while other parameter showed moderate variation. Of the qualitative characters studied, a high coefficient of variation was measured for anthocyanine content (30.03%) and Tacidity content (8.04%). Moderate coefficient of variation was observed for Carotinoids (7.16%), TSS/TA (7.63%) and Ascorbic acid (6.96%) content while other parameter showed lower variation. Highest anthocyanine content (0.41 mg/100g) and lowest (0.09mg/100g) was noticed in Gp 33 and Gp 44, respectively while highest carotenoids content was recorded in Gp 44 (17.36 mg/100g) and lowest in Gp 51 (1.30 mg/100g). The present study showed high to moderate physiochemical especially high ascorbic acid, flavonoids, carbohydrate and carotenoids content suggested that the Mango can be used as a good source preparation of health drink. The germplasm may be important commercial mango fruit, grown and available almost everywhere in Bangladesh, can help overcoming the malnutrition problem in our country.

Knowledge Management: Journal