Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Effect of Water Ph on the Early Developmental Responses in Zebrafish (danio Rerio)

MM Zahangir
Department of Fisheries Biology & Genetics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

MS Islam*
Department of Fisheries Biology & Genetics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

F Haque
Department of Fisheries Biology & Genetics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

GM Mustakim
Department of Fisheries Biology & Genetics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

H Khatun
Department of Fisheries Biology & Genetics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Abstract/ Executive Summary:

This experiment was conducted to evaluate the egg production performance in zebrafish (Danio rerio) in different pH treatments. Zebrafish were reared continuously 15 days in 3 different pH treatments like control (pH 6.8-7.4), acidic (pH 5.0-6.0) and basic (pH 9.0-10.0) media and then eggs were collected from them. Numbers of egg collection from the acidic and basic media were significantly varied from the control. Collected eggs from acidic, basic and control media were then incubated at different pH treatments ranging from pH 2.0- 12.0. Significant differences were observed in hatching rate and time in control medium from two other different treatments.

Keywords: Water, pH, zebrafish

Location: Department of Fisheries Biology & Genetics, Bangladesh Agricultural University, Mymensingh

Start Date:

End Date:

Program Area: Animal Health and Management

Commodity:

Objectives:

The present study addresses the effects of different pH on egg production and embryonic development of the zebrafish

Methodology:

Wild-type matured zebrafish (Danio rerio) were collected from the Field Laboratory Complex of the Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh and maintained at 27± 1°C, at a density of one fish per litre of water in a 60 litre water tank in the backyard laboratory under this faculty. Zebrsfish were fed with zooplankton and commercial pelleted feed (Krishibid Fish Feed Ltd.) twice a day. Water exchange and tank-cleaning were made as required. The same mature fish were used repeatedly for experiments. To ensure fertility, each fish was initially permitted to spawn naturally. A total of 10 pair of fertile zebrafish was used in a ratio of 1:1 in each aquarium. Matured male and female fish were reared in each aquarium for a period of 15 days to adjust with this environment. After that they were provided with plastic tray with two layers of marble and ornamental plastic trees to prevent the fish eating their eggs once laid (Matthews et al., 2002). Thus ‘breeding traps’ were provided to prevent them from eating the eggs laid. Female zebrafish release eggs directly onto a bare substrate, but when provided with an artificial spawning site, such as a plastic box filled with gravel or marbles, they will preferentially use this (Spence et al., 2006). The fish usually laid eggs in the early morning of the following day. The naturally-spawned and fertilized eggs were transferred into petridish containing water. The eggs were counted from each aquarium and checked under a digital camera equipped microscope (Optika, Italy). Zebrafish embryos were then incubated at pH 2.0-12.0 from each treatment for the determination of hatching performances. The desired pH was prepared by adding acetic acid CH₃COOH) and sodium hydroxide (NaOH) with the help of a portable pH meter (HANNA, HI98107 ROMANIA) and was adjusted twice in a day. All the experiments were repeated thrice, using different batches of embryos. The effect of pH exposure of embryos was assessed in terms of cleavage rate and pattern, gastrulation and hatching. The results represent the mean of three replicates per pH treatments. The standard error of mean (SEM) was also presented.

Source of Information: Progressive Agriculture 26:85-89, 2015 ISSN: 1017 - 8139

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

pH contamination to the environment, which has yet not been recognized as a serious pollution problem, is capable of exerting considerable biological effects in nature. In the present study the data revealed that very low and very high pH effects on the early developmental responses to the test organisms.

Knowledge Management: Journal