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Induction of Resistance in Rice Against Sheath Blight Due to Seed Treated with Bio-extracts

Dr. M. A. Kashem
Principal Scientific Officer
Plant Pathology Division Banglaseh Institute of Nuclear Agriculture BAU Campus, Mymensingh-2202

Abstract/ Executive Summary:

The effect of different bio-extracts such as; Rhizoctonia solani, Trichoderma sp. mixture of R. solani + Trichoderma sp. fruit and leaf of datura, bacillus and fungicide (Provax) were assessed against sheath blight of rice under laboratory and pot condition. To induce resistance in rice against sheath blight, the bio-extracts were applied in seeds in laboratory condition. Then seedlings were transplanted in pot and data were taken at seedling and maximum tillering stages. The highest 87.2% germination was found in suspension of Trichoderma and the lowest 46.6% in untreated control. Root length, shoot length, root weight and shoot weight were found higher in mixture of R. solani +Trichoderma sp. and lower in untreated control. At maximum tillering stage, the lowest 30.00 mm (15 days after inoculation, DAI) lesion length, the highest 93.28% healthy tiller and 74.25 cm plant height were observed in mixture of R. solani + Trichoderma sp. and all were the lowest in control. The highest 59.06% infected tiller was also recorded in untreated control.

Keywords: Sheath blight, Bio-extracts, Resistance, Rice

Location: Bangladesh Institute of Nuclear Agriculture

Start Date: 02-03-2011

End Date: 30-08-2011

Program Area: Pest Management

Commodity: Rice

Objectives:

To induced resistance in rice against sheath blight using bio-extract.

Methodology:

Different bio-agents and plant parts were collected from around the BINA office. Bio-extracts were prepared at the Laboratory of Plant pathology Division, BINA and applied in laboratory and pot experiment during March to August 2011. The seeds of T. aman rice line (TN-1), susceptible to sheath blight was collected from BINA, Mymensingh. The experiments was laid out in a Completely Randomized Design (CRD) with four replications. Different treatments prepared in the laboratory were used in pot at suspension of Trichoderma sp. macerated extract of Trichoderma sp. mixure of R. solani + Trichoderma sp., datura fruit extract, provax (fungicide) and macerated extracts of bacteria. Each suspension was prepared with a ratio of 1:10. For seed germination, fifty petridishes were cleaned and four blotter papers were set in each petridish. Twenty five seeds were set in each petridish. A total of 40 pots were prepared, filling with 8 kg/pot of air dried soil,. Seven days old seedlings were transplanted in the pot. Three seedlings were transplanted in each pot. Intercultural operations were done as and when necessary. The plants were inoculated with mycelial block of 5 mm diameter and 10 days old culture of R. solani. Different parameters such as; germination percentage, root length, shoot length, root weight, shoot weight, lesion length, disease severity, number of infected tiller, number of healthy tiller and plant height were collected.

Source of Information: Bangladesh J. Nuclear Agric. 23 & 24: 77-81, 2012

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The lowest lesion and the highest healthy tiller were observed in R. solani + Trichodema sp. mixure.

Knowledge Management: Journal