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Comparative Study of Nonnodulated and Nodulated Chickpea Genotypes in Respect of Some Biochemical Parameters as Affected by Sulphur Deficiency

Dr. M. Badruddin
Senior Scientific Officer
Crop Physiology Division, Bangladesh Institute of Nuclear Agriculture, Mymensingh, Bangladesh

Abstract/ Executive Summary:

An experiment was conducted to observe the effects of sulphur deficiency on some biochemical activities in nonnodulated and nodulated chickpea genotypes in solution as well as in the sand in pots. Results reflected that nitrate accumulation increased in the seedlings of the nonnodulated and nodulated genotypes but that was decreased, due to S-deficiency, in the root and leaf in early stage of growth. S-deficiency caused decrease in nitrate reductase (NR) activity in both the genotypes and that it showed different rhythmic patterns between the genotypes in their ontogenetic development. Soluble protein accumulation was also affected due to S-deficiency in both the genotypes. It increased accumulation of sugar (reducing and total) in the root but that was decreased in the leaves. S-deficiency resulted increase in total amino acids in both roots and leaves of the genotypes studied. The above findings reflected that most of the biochemical characters in chickpea were affected at S-deficiency and that variability in the biochemical parameters recorded in the nonnodulated and nodulated genotypes.

Keywords: Amino acid, NO2-, NR activity, S-deficiency, Soluble protein

Location: Pot yard of BINA, Mymensingh

Start Date: 07-11-1999

End Date: 01-04-2000

Program Area: Crop-Soil-Water Management

Commodity: Chickpea

Objectives:

To observe the effects of sulphur deficiency on some biochemical parameters in chickpea genotypes.

Methodology:

Surface sterilized seeds were soaked in distilled water for 24 hour and allowed to germinate. Germinated seeds were grown in Latcombe nutrient solution (Hewitt 1966) in a growth cabinet for 15-d. Nutrient solution was replenished at every 3-d. Nitrate reductase (NR) activity was determined following Stewart and Orebamjo (1979) at 2-, 3-, 4-, 5-, 6-d after addition of 5 mM nitrate in both S-supplied and S-deficient seedlings. In a separate experiment, plants were grown in sand using the same nutrient solution and NR activity was determined in 2-, 3-, 4-, 5-, 6-, 7- and 8-week old, S-deficient and S-supplied plants of both the genotypes to observe ontogenetic pattern of NR activity. In this experiment, 5 mM nitrate was applied in plants before 48 h of each determination. NR activity was expressed as µmol NO₂^-/gfw/h.

For other biochemical analysis, chickpea seedlings were grown in Latcombe solution. Sulphur deficiency was applied to 15-d old seedlings. Samples were collected after 1-, 3- and 5-d of sulphur treatments. Fresh samples of root and leaves were analyzed for soluble protein (Lowry et al. 1951), nitrate (Cataldo et al. 1975), total sugar (Dubois et al. 1956) and reducing sugar following Nelson (1944) and Somogyi (1952). Total amino acid was determined using methoxyethanol and citrate buffer. Soluble protein, total sugar, reducing sugar and amino acid were expressed as milligram gram^-1 fresh tissue and nitrate was expressed as µgram gram^-1 fresh tissue.

Source of Information: PhD Thesis by M. BUDRUDDIN, Department of Botany, Session:1997-98, Roll # 03, Thesis accepted on July 2005. Thesis Serial # Bot 215 (Department Library). The Thesis also Available at BINA Lireary # C12123, pp. 98-115

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Chickpea genotypes differ in respect of biochemical characters. NR activity and soluble synthesis were highly affected due to S-deficiency.

Knowledge Management: Thesis